Proteinase K: Activity: >30 units/mg protein (hemoglobin, pH7.5, 37oC)
Unit definition: It is the amount of enzyme which releases at 37 oC in 1 min as many folinpositive amino acid and peptides from hemoglobin as 1umol of tyrosine.
Features: Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.
Usage: In presence of 0.5-1.0% SDS Proteinase K inactivates DNases and RNases in eukaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.
Preparation Instructions: This product is soluble in double distilled water (1 mg/ml), yielding a clear colorless solution.
Quality:
Purified by chromatography and lyophilized
RNases: Not detectable
DNases: Not detectable
Exonucleases: Not detectable