G-Biosciences' Protein G Magnetic Beads are Fe3O4beads coated with dextran to produce highly uniform, 1µm beads. Recombinant Protein G is covalently coupled to the dextran coat to produce an enhanced alternative to agarose slurries for immunoprecipitation experiments. G-Biosciences magnetic beads compare well to leading magnetic beads (Figures 2 & 3).
The use of magnetic beads offers several distinct advantages of traditional immunoprecipitation experiments include a significant reduction in time and non-specific protein binding.
A simple protocol involves the addition of your antibody of choice to the beads, which bind the Fc region (See Table 1) during a short incubation. The tube is placed on a magnet and the supernatant is removed by aspiration. The antibody bound magnetic beads can be used in a variety of downstream processes including:
- Immunoprecipitations
- Co-immunoprecipitations
- Chromatin immunoprecipitation (ChIP)
- Small-scale IgG Purification and antibody labeling
- Protein Isolation
Features
- 1µm beads
- 260µg human IgG/ml
- Significant reduction in background due to non-specific binding
- Simple, short procedure
- No costly columns required
- No time consuming centrifugations required
- Gentle separation preserves native protein interactions
Applications
- Immunoprecipitations
- Co-immunoprecipitations
- Chromatin immunoprecipitation (ChIP)
- Small-scale IgG Purification and antibody labeling
- Protein Isolation