Percoll® is a medium for density gradient centrifugation of cells, viruses and subcellular particles. Percoll® is composed of colloidal silica coated with polyvinylpyrrolidone (PVP), which renders the material completely non-toxic and ideal for use with biological materials. Centrifugation of Percoll® results in spontaneous formation of a density gradient due to the heterogeneity of particle sizes in the medium. Percoll® can be used for formation of gradients either by the use of conventional gradient mixers or by high speed centrifugation. In the latter case the sample can be premixed with the medium and then separated on the gradient created in situ. In this way gradient formation and sample separation can be achieved in a single operation.
Key Applications: Cell fractionation, density gradient, protein purification
Product Type: Proteins, Enzymes & Peptides
Density: 1.128 g/mL(lit.)
Viscosity: Record cp at 20°C
Presentation: Yellow Liquid
Format: Liquid
pH: 8.9 ± 0.3 at 20°C (lit.)
NOTES:
Osmolality Max 25 mOsm/kg
Storage & Handling: +4°C