Pefabloc

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Pefabloc
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  • ROCHE Pefabloc® SC PLUS

    MilliporeSigma

    …description: The Pefabloc ® SC PLUS set combines the serine protease inhibitor Pefabloc ® SC with a uniquely formulated Pefabloc ® SC protector (PSC-Protector), adding additional convenience and reliability to the safe, stable protease inhibition provided by Pefabloc ® SC .…

  • ROCHE Pefabloc® SC PLUS

    MilliporeSigma

    Pefabloc SC is a specific, potent, and irreversible inhibitor of serine proteases. The inhibitory activity is comparable to PMSF or DFP, but Pefabloc SC is nontoxic to cells. The Pefabloc SC PLUS set combines the serine protease inhibitor Pefabloc SC with a uniquely formulated Pefabloc SC…

  • ROCHE Pefabloc® SC

    MilliporeSigma

    …delay in thrombin inactivation. Pefabloc SC, however, does not react with serum albumin, and exhibits a threefold higher capacity to inactivate thrombin under similar conditions. • Pefabloc SC can be used on living cells. • Use Pefabloc SC to inactivate proteinase K, for…

  • …in cell culture in concentrations of up to 0.25-ámM. Other Notes: Potent and irreversible inhibitor of serine proteases; in contrast to PMSF, it is water soluble and non-toxic 1, 2 ; Unaffected by albumin 3 Legal Information: Pefabloc is a registered trademark of Pentapharm

  • Synonym(s): 4-Amidino-Nα-(naphthalene-2-sulfonylglycyl)-DL-phenylalanine-piperidide acetate salt; NAPAP Empirical Formula: C27H31N5O4S -+ C2H4O2 Linear Formula: …

  • … Antipain dihydrochloride, 3 mg; Bestatin, 0.5 mg; Chymostatin, 1 mg; E-64, 3 mg; Leupeptin, 0.5 mg; Pepstatin, 0.5 mg; Phosphoramidon, 3 mg; Pefabloc SC, 20 mg; EDTA, 10 mg, disodium salt; Aprotinin, 0.5 mg RIDADR: NONH for all modes of transport Application: The protease inhibitors in…

  • …may only have a half-life of 35 min. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative. Quality: Purity: Chromatographically homogeneous Preparation…

  • …try our Protease Inhibitor Set including Antipain Dihydrochloride, Aprotinin, Bestatin, Chymostatin, E-64, EDTA-Na2, Leupeptin, Pefabloc SC, Pepstatin, and Phosphoramidon. Biochem/physiol Actions: Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain,…

  • …and guanidinium salts. Inhibitors: The enzyme is inactivated by Pefabloc ® SC. However, it is not inactivated by metal ions, chelating agents (e.g., EDTA), sulfhydryl reagents, or trypsin and chymotrypsin inhibitors. Legal Information: Pefabloc is a registered trademark of Pentapharm

  • …contain denaturing reagents such as urea, sodium dodecyl sulfate (SDS), and guanidinium salts. Inhibitors: The enzyme is inactivated by Pefabloc® SC. However, it is not inactivated by metal ions, chelating agents (e.g., EDTA), sulfhydryl reagents, or trypsin and chymotrypsin inhibitors.…

  • …nucleases such as RNases and DNases. The enzyme is also available as a solution. • I nhibitors : The enzyme is inactivated by Pefabloc ® SC. However, it is not inactivated by metal ions, chelating agents (e.g., EDTA), sulfhydryl reagents, or trypsin and chymotrypsin inhibitors.…

  • …In addition, FOCUS™ ProteaseArrest™ uses PMSF as its primary serine protease inhibitor as opposed to the commonly used Pefabloc®. Pefabloc® has been reported to modify proteins at high concentrations and result in artifacts in subsequent 2D electrophoresis and mass spectrometry.…

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