Pbs Cell Culture

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Pbs Cell Culture
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  • Nu-Serum™ IV Growth Medium Supplements

    Corning

    …routinely used for cell culture. Replaces fetal bovine and other sera on an equivalent volume basis. Quality Tested at a concentration of 10% for the ability to stimulate a =100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells Tested…

  • Nu-Serum™ Growth Medium Supplements

    Corning

    …routinely used for cell culture. Replaces fetal bovine and other sera on an equivalent volume basis. Quality Tested at a concentration of 10% for the ability to stimulate a = 100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells Tested…

  • SIGMA Macrophage Inflammatory Protein-3a human lyophilized powder (from PBS…

    MilliporeSigma

    Synonym(s): hMIP-3α; Exodus; LARC; MIP-3α MDL Number: MFCD01635053 Purity: ≥95% (SDS-PAGE) Storage: −20°C

  • Anti-VSV-Glycoprotein-Agarose antibody, Mouse monoclonal, clone P5D4, purified…

    MilliporeSigma

    …carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain…

  • PBS Tablets

    MP Biomedicals

    …in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism;…

  • 20X PBS Solution, pH 7.4

    Growcells

    …applications. Ultra pure quality makes buffer ideal for use in a variety of cell culture procedures, such as washing and transporting cells or tissue samples, diluting cell for counting and general preparation of reagents. PBS contains no Ca or Mg ions to provide efficient washing of chelators. Sold…

  • HyClone™ Phosphate Buffered Saline

    GE Healthcare

    Buffer cell culture growth with GE Healthcare HyClone Phosphate Buffered Saline (PBS), a QC-tested solution. Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities Full traceability and documented origin of all formula ingredients Validated and consistent production…

  • Trypan Blue Solution (w/v) in PBS

    Corning cellgro

    …in cell count and viability assays.  Viable cells exclude the dye, while nonviable cells absorb the dye and appear blue.  Cells should be in suspension as single cells in buffered saline before counting.  Trypan blue can be used in assays providing total cell count, viable cell cell

  • Accumax(TM) solution, sterile-filtered, suitable for cell culture

    MilliporeSigma

    …assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware…

  • Trypan Blue 0.4% Solution in PBS

    MP Biomedicals

    Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays.

  • HyClone™ Dulbecco's Phosphate Buffered Saline, Powder

    GE Healthcare

    Buffer cell culture with GE Healthcare HyClone Dulbecco's Phosphate Buffered Saline (PBS), powder.

  • Accutase(R) solution, sterile-filtered, suitable for cell culture

    MilliporeSigma

    …tumor cell lines, and insect cells. Performs exceptionally well in detaching cells for analysis of cell surface markers, virus growth assay, and flow cytometry as well as bioreactor scale-up. Does not contain mammalian or bacterial-derived products. Formula variant: Prepared in Dulbecco's PBS

  • PBS (Phosphate Buffered Saline), 10X, pH 7.4

    Growcells

    …applications. Ultra pure quality makes buffer ideal for use in a variety of cell culture procedures, such as washing and transporting cells or tissue samples, diluting cell for counting and general preparation of reagents. PBS contains no Ca or Mg ions to provide efficient washing of chelators. Sold…

  • PBS (Phosphate Buffered Saline), 1X, pH 7.4

    Growcells

    …applications. Ultra pure quality makes buffer ideal for use in a variety of cell culture procedures, such as washing and transporting cells or tissue samples, diluting cell for counting and general preparation of reagents. PBS contains no Ca or Mg ions to provide efficient washing of chelators. Sold…

  • HyClone™ Dulbecco's Phosphate Buffered Saline

    GE Healthcare

    Buffer cell culture with GE Healthcare HyClone Dulbecco's Phosphate Buffered Saline (PBS), solution. Quality DPBS solutions in the concentrations and volumes to meet your needs. Available with or without calcium and magnesium dependent on your Trypsin inhibition requirements. Whether it is…

  • bioPLUS PBS (Phosphate Buffered Saline) Tablets

    bioWORLD

    …stirring. Technical Information: One tablet prepares 100mL of PBS Higher concentrations can be obtained by dissolving 2, 3 or 4 tablets per 100mL of water To sterilize the solution for cell culture, simply autoclave or sterile filter. Contents: Sodium Chloride 137mM; Phosphate…

  • Laminin from human placenta, liquid, BioReagent, suitable for cell culture

    MilliporeSigma

    …solution slowly before use at 2-8C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8C.

  • SIGMA Stem Cell Factor human, recombinant, expressed in E. coli, powder,…

    MilliporeSigma

    … Physical form: Lyophilized from a 0.2 μm filtered solution in PBS containg 50 μg BSA /μg cytokine. Analysis Note: The proliferative activity is tested in culture using TF-1 cells or by the dose dependent proliferation of human MO-7e cells. Storage: −20°C

  • SIGMA NGF-B from rat, recombinant, expressed in Sf21 cells, lyophilized powder,…

    MilliporeSigma

    …μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine Analysis Note: The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1. Storage: −20°C

  • SIGMA Cystatin C human, recombinant, expressed in HEK 293 cells, HumanKine®,…

    MilliporeSigma

    …(glomerular filtration rate, GFR). General description: HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells and has an apparent molecular mass of 12-13 kDa. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C…

  • ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, liquid, BioReagent, suitable…

    MilliporeSigma

    …below 10C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium,…

  • ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, liquid, BioReagent, suitable…

    MilliporeSigma

    …below 10C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium,…

  • SIGMA Interleukin-6 human, recombinant, expressed in E. coli, lyophilized powder…

    MilliporeSigma

    Synonym(s): hIL-6; IL-6 Purity: ≥97% (SDS-PAGE) Physical form: Lyophilized from a 0.2 μm filtered solution of phosphate buffer saline (PBS), pH 7.4, containing 500 μg bovine serum albumin. Storage: −20°C

  • Monoclonal Anti-Alkaline Phosphatase, Human Placental-Agarose antibody produced…

    MilliporeSigma

    …has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper) Application: Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase,…

  • Monoclonal Anti-Biotin-Agarose antibody produced in mouse, clone BN-34, purified…

    MilliporeSigma

    …to human and rabbit antigens coated on multiwell plates. General description: Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays…

  • Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 1-2 mg/mL…

    MilliporeSigma

    …this solution slowly before use at 2-8C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8C.

  • Trypsin-EDTA solution, 1 x, sterile-filtered, BioReagent, suitable for cell…

    MilliporeSigma

    … The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Biochem/physiol Actions: Trypsin cleaves peptides on the…

  • ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, growth-factor reduced,…

    MilliporeSigma

    …below 10C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium,…

  • SIGMA Dulbecco's Phosphate Buffered Saline, Modified, without calcium chloride…

    MilliporeSigma

    Synonym(s): DPBS Application: Dulbecco’s Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water (W3500). D-PBS is used to wash cells during preparation and serial transfer.

  • SIGMA Dulbecco's Phosphate Buffered Saline, Modified, without calcium chloride…

    MilliporeSigma

    Synonym(s): DPBS Application: D-PBS is used to wash cells during preparation and serial transfer. Other Notes: This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available Storage: 2-8°C

  • ROCHE Cell Proliferation ELISA, BrdU (colorimetric)

    MilliporeSigma

    …1:100 with sterile culture medium (resulting concentration: 100 μM BrdU). For one 96-well MP, 1 ml BrdU labeling solution is required if the cells were cultured in 100 μl /well (10 μl/well) and 2 ml BrdU labeling solution is required if the cells were cultured in 200 μl/well…

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