Np 40 Detergent

…Fluorescent Detergent Assay Calculate detergent levels Monitor Detergent Removal Suitable for most commonly used detergents, including Tween 20 & 80 SDS CTAB NP-40 Brij 35 & 58 Triton X-100 Sodium Deoxycholate Applications Monitor detergent removal and…

Laboratory reagent grade. Nonidet P-40 is a nonionic, non-denaturing detergent.

…globulin protein stand ard. A ML (Modified Lowry) Protein Assay is available for detergent containing samples without reducing agents.The ML Protein Assay is compatible with a wide variety of detergents used in protein research in addition to other common reagents such as EDTA and Tris(see…

…Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The ML Protein Assay is compatible with a wide variety of detergents used in protein research in addition to other common reagents such as EDTA and Tris(see tables 1 and 2).The assay is supplied with either traditional…

…biological activity. We recommend using 1.0 ml of RIPA Lysis Buffer to lyse 0.5 to 5 x 10E7 adherent mammalian cells. This buffer contains ionic detergents and may not be suitable for kinase enzymes, if these enzymes are easily denatured. Do not add phosphatase inhibitors when preparing lysates…

…biological activity. We recommend using 1.0 mL of RIPA Lysis Buffer to lyse 0.5 to 5 x 10E7 adherent mammalian cells. This buffer contains ionic detergents and may not be suitable for kinase enzymes, if these enzymes are easily denatured. Do not add phosphatase inhibitors when preparing lysates…

…biological activity. We recommend using 1.0 ml of RIPA Lysis Buffer to lyse 0.5 to 5 x 10E7 adherent mammalian cells. This buffer contains ionic detergents and may not be suitable for kinase enzymes, if these enzymes are easily denatured. Do not add phosphatase inhibitors when preparing lysates…

…from chromatin-antibody-bead complexes. The specially formulated ChIP DNA Binding Buffer promotes DNA adsorption to the column in the presence of detergents, antibodies, and proteinases that are often used for ChIP. This kit may be applied to any routine ChIP procedure to determine DNA concentration…