Luciferase

Synonyms: Luciferase firefly MDL Number: MFCD00131546 Storage: -20C Application: Firefly luciferase is used extensively in molecular and cell biology, in particular for the efficient detection and quantitation of ATP and as a reporter for genetic function. Biochem Physiol Actions: …

Luciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. Firefly luciferase refers to the enzyme that was isolated from Photinus pyralis. This lentivirus expresses Firefly luciferase under the control of CMV promoter.

Luciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. Firefly luciferase refers to the enzyme that was isolated from Photinus pyralis. This lentivirus expresses Firefly luciferase under the control of UBC promoter.

Luciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. Firefly luciferase refers to the enzyme that was isolated from Photinus pyralis.

Luciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. Renilla luciferase refers to the enzyme that was isolated from the sea pansy, Renilla reniformis.

…of site specific recombinase and it is known to catalyse the site specific recombination event between two DNA recognition sites (loxP sites). This premade lentivirus expresses Cre Recombinase under the control of PGK promoter, and Renilla luciferase reporter under the control of CMV promoter.

…of site specific recombinase and it is known to catalyse the site specific recombination event between two DNA recognition sites (loxP sites). This premade lentivirus expresses Cre Recombinase under the control of PGK promoter, and Firefly luciferase reporter under the control of CMV promoter.

Firefly Luciferase Assays are one of the widely used reporter assays to detect and measure gene expression, function and regulation. G-Biosciences Lumino™ Firefly Luciferase Assay is designed for rapid and high sensitive detection or quantification of firefly luciferase reporter enzyme…

…for substrate injection. Emitted light is recorded by a standard luminometer at 560nm. Luciferase assay kit contains optimized components (cell lysis buffer, luciferase substrate and Luciferase Assay Buffer) to ensure maximal sensitivity with a consistent light output. Very sensitive…

…band purity Protein amount: determined based on absorbance at 280 nm and molecular extinction co-efficeint of Luciferase Source: E. coli Shipping and hand ling: Luciferase (Firefly, Recombinant) is shipped on dry ice. Store at -20°C. Avoid repeated thawing and thaw on ice. When…

A light-generating enzyme that catalyzes an oxidative reaction, using luciferin, ATP, and molecular oxygen to produce oxyluciferin, which yields visible light.

…order (5 or more) of luciferase concentration. The linear range is also dependent of the sensitivity of the luminometer. Assay reagents compatible with other firefly luciferases. Application(s): Rapid and high sensitive detection or quantification of firefly luciferase reporter enzyme…

Myco-Sniff-Rapid Mycoplasma Luciferase Detection Kit offers a rapid (20 mins), simple and sensitive method for detection of mycoplasma contamination in cell cultures. This innovative kit utilizes the activity of mycoplasma metabolic enzymes from most mycoplasma species, including those commonly…

A synthetic cyclic alkylaminoluciferin that exhibits properties that are superior to those of D-luciferin. Binds to luciferase with higher affinity and and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a…

…quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells. High sensitivity and wide detection range. Can be readily automated on HTS liquid handling systems. The three step assay allows detection of dual luciferase levels within 20 minutes. Method:…

…rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens (for 500 assays). High sensitivity and wide detection range. This fast and homogeneous "mix-and-measure" assay allows detection of luciferase levels within 10 minutes.…

This NF-kB reporter lentivirus expresses Firefly luciferase under the control of a minimal (m)CMV promoter and tandem repeats of the NF-kB transcriptional response element (TRE). It can be used to monitor the transcriptional activity of NF-kB.

This STAT3 reporter lentivirus expresses Firefly luciferase under the control of a minimal (m)CMV promoter and tandem repeats of the STAT3 transcriptional response element (TRE). It can be used to monitor the transcriptional activity of STAT3.

This STAT3 reporter lentivirus expresses Firefly luciferase under the control of a minimal (m)CMV promoter and tandem repeats of the STAT3 transcriptional response element (TRE). It can be used to monitor the transcriptional activity of STAT3.

…system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens. High sensitivity and wide detection range. This fast and homogeneous "mix-and-measure" assay allows detection of luciferase levels within 10 minutes. The…

Storage: −70°C

The MISSION(R) Luciferase shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting luciferase from Photinus pyralis (GenBank Accession No. M15077). The Luciferase shRNA Control Vector is useful as a positive knockdown control in experiments using…

When conducting experiments using MISSION(R) shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction…

…Renilla luciferase activity in the same sample with high sensitivity and linearity. Firefly luciferase activity is measured first, then Renilla Luciferase Assay Buffer 2.0 is added to simultaneously quench firefly luciferase activity and measure Renilla luciferase activity. Renilla Luciferase Assay…

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Firefly luciferase is a 62,000 Dalton protein,…

Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays. The enzyme does not require post-translational…

Synonym(s): Bacterial Luciferase; Luciferase from Photobacterium fischeri MDL Number: MFCD00131545 Storage: −20°C

…can be assessed based on the amount of ATP available. The ATP Cell Viability Luciferase Assay offers a highly sensitive homogenous assay for quantifying ATP in cell cultures. This kit takes advantage of Firefly luciferase's use of ATP to oxidize D-Luciferin and the resulting production of light in…

Synonym(s): Luciferase firefly MDL Number: MFCD00131546 Storage: −70°C