Used as a differential method to detect the difference between those organisms which decolorize with treatment of acid alcohol, and those that do not, such as the mycobacteria. This is the "Cold Stain" method that does not require heating of the slide. Kit includes one 8 oz. bottle of each of the following: Carbol Fuchsin (Kinyoun) Acid Alcohol (Decolorizer) Methylene Blue (Counterstain).