Ready To mix.
Kit consists of:
2x10 ml ampoules 16% Formaldehyde1x10 ml ampoules 50% Glutaraldehyde1x50 ml 0.2M Sodium Phosphate Buffer.
Mixture 1: (Original Karnovsky's Mixture)
- 16% Paraformaldehyde Solution: 20ml
- 50% Glutaraldehyde EM Grade: 8ml
- 0.2M Sodium Phosphate Buffer: 25ml
- Distilled Water: 25ml
Final mixture is 78ml with 5% Glutaraldehyde, 4% Formaldehyde in 0.08M buffer. This mixture is extremely hypertonic, with an osmolarity of more than 2000m OSM .
Mixture 2:
A lower concentraction of Glutaraldehyde and Formaldehyde especially used for vascular profusion.
- 16% Paraformaldehyde Solution: 13ml
- 50% Glutaraldehyde EM Grade: 5ml
- 0.2M Sodium Phosphate Buffer: 50ml
- Distilled Water: 32ml
Final mixture is 2% Paraformaldehyde, 2.5% Glutaraldehyde and 0.1M Buffer. If necessary, the osmolarity can be adjusted by adding sucrose to NaCl. The pH may also need to be adjusted to your desirable point. This mixture is the most effective general fixative for both plant and animal specimens.
CAS #: 50-00-0, 7778-77-0, 50-00-0, 7732-18-5, 7778-77-0, 7732-18-5