Iptg X Gal

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Iptg X Gal
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  • X-GAL & IPTG Combo

    RPI (Research Products International)

    Special savings for ordering X-GAL and IPTG at the same time! Package includes 1 gram bottle of X-Gal and a 1 gram bottle of IPTG.

  • … instructions, Linearized pUC plasmid & DNA fragment, T4 Ligase, BactoBeads™ for transformation, reconstitution buffer, XGal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour™ Luria Broth Agar, Luria broth media for recovery, growth media, assay components, plastic supplies …

  • X-GAL & IPTG, Ready to Use, Non-Toxic Solution

    RPI (Research Products International)

    Stable solution of X-GAL and IPTG. Does not contain DMSO or DMF. Solution concentration is 10 mg/ml X-Gal and 8 mg/ml IPTG. Shelf Life: 1 Year

  • …active β-galactosidase produce red colonies when grown on plates containing magenta-gal. Magenta-gal is commonly used in conjunction with IPTG for red/white screening. Specifications: 5-Bromo-6-chloro-3-indoxyl β-D- galactopyranoside Magenta™-β-D-galactoside;…

  • IPTG (isopropylthiogalactoside) is an inducer of the lac operon in bacteria, which is frequently used in cloning as a component of a recombinant plasmid.  When used with X-gal to detect lac gene activity, IPTG can be used for screening of clones containing a gene of interest in the lac operon. …

  • …inducer of beta-galactosidase used to promote the expression of proteins in cells controlled by the lac operator system. When using blue/white screening, 0.1 mM final IPTG concentration in LB (Luria Broth) media is recommended. Isopropyl-?-D-Thiogalactopyranoside ( IPTG ) is usually used with X gal.

  • IPTG

    RPI (Research Products International)

    IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene. Dioxane-Free Water…

  • X-GAL

    RPI (Research Products International)

    Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The…

  • The IPTG/X-GAL Solution is a ready-to-use product in an easy-to-use spray bottle format. The solution concentration is 8mg/ml. Each pump of the spray bottle produces about 0.2ml of solution.

  • IPTG

    IBI Scientific

    IPTG is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to detect lac gene expression in cloning applications, thus allowing detection of recombinant molecules.

  • IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene. Dioxane-Free Water…

  • X-Gal

    ACTGene, Inc.

    …b-Galactosidase activity in the presence of IPTG and X-Gal containing media. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of b-Galactosidase activity leading to the appearance of white colonies on X-Gal and IPTG containing media. Non recombinant…

  • …β-galactosidase induction and the detection of micrometastasis formation during tumor progession.   X-gal is commonly used in conjunction with IPTG for blue/white screening.   Specifications : MOLECULAR BIOLOGY GRADE Purity: Greater than 99% (HPLC) Solution: …

  • DTT

    Soltec Bio Science

    …inducer of beta-galactosidase used to promote the expression of proteins in cells controlled by the lac operator system. When using blue/white screening, 0.1 mM final IPTG concentration in LB (Luria Broth) media is recommended. Isopropyl-?-D-Thiogalactopyranoside ( IPTG ) is usually used with X gal.

  • IPTG (Isopropyl-β-D-thiogalactopyranoside) is a galactose analogue not recognized by β-galactosidase. IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. IPTG is a galactose analogue not recognized by β-galactosidase. It…

  • Synonyms: IPTG; Isopropyl β-D-thiogalactoside Formula: C9H18O5S Formula Weight: 238.30 CAS No.: 367-93-1 Purity: ≥99% (TLC) Storage Temperature: 2-8°C Application: IPTG is commonly used in cloning procedures that require induction of…

  • Synonyms: IPTG; Isopropyl β-D-thiogalactoside Formula: C9H18O5S Formula Weight: 238.30 CAS No.: 367-93-1 Purity: ≥99% (TLC) Storage Temperature: 2-8°C Application: IPTG is commonly used in cloning procedures that require induction of…

  • IPTG (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. IPTG is a commonly used reagent in cloning procedures that require induction of…

  • Synonyms: Isopropyl β-D-1-thiogalactopyranoside; iptg; IPTG; Isopropyl β-D-thiogalactoside Empirical Formula (Hill Notation): C 9 H 18 O 5 S Formula Weight: 238.3 Linear Formula: C 9 H 18 O 5 S MDL Number: MFCD00063273 Storage: 2-8°C Beilstein Registry Number: …

  • …solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months. Specifications Assay Time: 50 minutes Specific Activity: The described standard assay will results in a specific activity of 1.8 x 10 9 dpm/μg, corresponding to 65% incorporation…

  • …active β-galactosidase produce blue colonies when grown on plates containing bluo-gal. Bluo-gal is commonly used in conjunction with IPTG for blue/white screening. Specifications: Bluo-Gal 5-Bromo-3-indolyl-β-D-galactopyranoside Blue-Gal, Lapis™-β-D-Gal…

  • …with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene…

  • …with ampicillin, nalidixic acid, penicillin and tetracycline. Blank discs are available to prepare your own screening discs. IPTG & X-Gal: IPTG (Isopropyl-b-D-thiogalactopyranoside) for chemical induction of regulated plasmids, X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside)…

  • …non-toxic solution For use in blue/white colony screening in expression studies Does not contain toxic DMF and DMSO Contains 40 mg/ml of IPTG (Isopropyl-β-D-Thiogalactopyranoside) and 40 mg/ml of X-gal (5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside) Supplied in a very easy…

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