Hepes Free Acid

HEPES, Free Acid is a zwitterionic buffer ideally suited for cell culture in a range of 10 to 25mM. HEPES has a buffering range from 6.8 to 8.2. HEPES is also a component of Tris-HEPES buffer for polyacrylamide electrophoresis of proteins.

…been published. Alternatively, equimolar concentrations of HEPES and of sodium HEPES can be mixed in approximately equal volumes, back-titrating with either solution to the appropriate pH. HEPES, free acid: HEPES, free acid HEPES has been described as one of the best all-purpose buffers…

Synonyms: 4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid , HEPES free acid Suitable for use in biopharmaceutical manufacturing applications. Formula: C8H18N2O4S MW: 238.31 g/mol Melting Pt: 210…215 °C Density: 1.448 g/cm³ (20 °C) Storage…

Zwitterionic N-substituted aminosulfonic acid buffer. pka (25°C): 7.5 pH Range (Working): 6.8 - 8.2 Heavy Metals: <0.001% Max

…The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium hydroxide to the desired pH. A simple mixing table for preparing 0.05 M HEPES/NaOH has been published. Alternatively, equimolar concentrations of HEPES and of HEPES sodium…

Formula: HOCH₂CH₂(C₄H₈N₂)CH₂CH₂SO₃H Formula Weight: 238.30 CAS Number: 7365-45-9 Synonym(s): N-2-Hydroxyethylpiperazine-N`-2-ethanesulfonic Acid

Features Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid CAS#: 7365-45-9 Molecular Formula: C₈H₁₈N₂O₄S Molecular Weight: 238.3  

For Liquid Chromatography and Molecular Biology Buffers Formula: C₈H₁₈N₂O₄S Formula Weight: 238.31 CAS Number: 7365-45-9 Synonym(s): (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)

…With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and…

Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) Empirical Formula: C₈H₁₈N₂O₄S Linear Formula: C₈H₁₈N₂O₄S Formula Weight: 238.30 …

… HEPES is an organic zwitterionic buffering agent effective in the physiological pH range of 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. This product has been qualified for use in cell culture. Preparation Note: Prepared from HEPES free acid…

…to 500% per cell have been observed. Other Notes: Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.…

…adaptation. Other Notes: This proprietary formulation includes inorganic salts, essential and non-essential amino acids, vitamins, sodium bicarbonate, HEPES, trace elements, fatty acids, and other organics. It contains low concentrations of recombinant human insulin, bovine serum albumin and…

… This animal-component free formulation is optimized for high protein production and well as superior expansion of CHO cells. Other Notes: Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human…

…resulting in increased productivity. Other Notes: Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, other organic compounds, trace elements, and surfactants. Does not contain…

…resulting in increased productivity. Other Notes: Proprietary formulation containing inorganic salts, HEPES and sodium bicarbonate buffers, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.…

…a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco’s Modified Eagle’s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate…

…growth of T and Blymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. C985G02, C985G03 and C985G04 is Iscove's Modified Dulbecco's Medium with L-glutamine and 25mM HEPES buffer. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37ºC prevents the initial rise…

…cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate…

…for extended periods resulting in increased productivity. Other Notes: Proprietary formulation containing inorganic salts, HEPES, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants. Does not…

…3,4-bisphosphate sodium salt; Phosphatidylinositol-3,4-diphosphate C-16 sodium salt Formula: C41H81O19P3 · xNa+ Formula Weight: 970.99 (free acid basis) Linear Formula: C41H81O19P3 · xNa+ Purity: >=98% Storage: -20C UNSPSC Code: 12352207 Biochem/physiol Actions: …

…amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures. C985F49 and C985F50 is Minimum Essential Medium with Earle's salts, L-glutamine and 25mM HEPES buffer. It does not contain sodium bicarbonate. HEPES, a zwitterionic…

…appearance and presence of particulate matter and haziness after dissolution. 2. Preparation of concentrated medium is not recommended since free base amino acids and salt complexes having low solubility may precipitate in concentrated medium. 3. pH and sodium bicarbonate concentration of the…