Hepes Buffer Solution

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Hepes Buffer Solution
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Hach

  • HEPES is an organic zwitterionic buffering agent effective in the physiological pH range of 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. This product has been qualified for use in cell culture. Preparation Note: Prepared from HEPES free…

  • …for Sigma by Boston Biochem., Inc. Biochem/physiol Actions: Protein activator of the 20S proteasome. Enhances the 20S proteasome hydrolysis of peptide substrates, but not hydrolysis of ubiquitinated proteins. Physical form: Solution in 50 mM Hepes, pH 7.6, 250 mM NaCl, 1 mM EDTA, and 1 mM DTT.

  • Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.

  • 1M HEPES Buffer

    MP Biomedicals

    …change the osmolality of the solution. Most solutions of HEPES hemisodium will dissolve in water forming a pH at 7.5 with little to no adjustment. HEPES buffer commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires…

  • Hepes Buffer Solution

    Electron Microscopy Sciences

    CAS #: 7365-45-9 pH range: 7.0-8.0

  • Synonyms: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid Formula: C8H18N2O4S Formula Weight: 238.30 CAS No.: 7365-45-9 Density: 1.07 g/mL at 20 °C

  • HEPES Buffered Saline Solution 2X

    RPI (Research Products International)

    2X Formulation: NaCI: 280 mM KCI: 10 mM Na2HPO4: 1.5 mM Dextrose: 12 mM HEPES: 50 mM pH: 7.0

  • HEPES Stock Solution (1 M)

    Cayman Chemical

    This bottle contains filtered HEPES (1 M), pH 7.5. The buffering range for HEPES is pH 6.8-8.2 and can be adjusted with KOH or NaOH. It is ready to use as supplied.

  • Components: Sodium Chloride(140 mM), Potassium Chloride(5 mM), HEPES(25 mM), Calcium Chloride Dihydrate(2 mM), Magnesium Chloride Hexahydrate(2 mM), Dextrose / Glucose(10 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4…

  • Components: Sodium Chloride(140 mM), Potassium Chloride(5 mM), HEPES(10 mM), Dextrose / Glucose(10 mM), Sodium Pyruvate(10 mM), BAPTA(2 mM) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • …material. Protocols & Applications: Ensure correct concentrations of buffer solutions with the Buffer Calculator Other Notes: Easily compare specifications for HEPES sodium salt products with the HEPES sodium salt specification table . Packaging: 1 kg in poly bottle Packaging:…

  • Buffers

    Corning cellgro

    HEPES HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2. Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the…

  • UltraSALINE A is a HEPES buffered saline used in trypsinization protocols of reduced-serum and serum-free systems and preparation of stock solutions.

  • Blasticidin S HCl Solution

    RPI (Research Products International)

    A peptidyl nucleaside antibiotic isolated from the culture broth of Streptomyces griseogenes, inhibits protein synthesis in both prokaryotes and eukaryates. Supplied as a 10mg/mL sterile solution in HEPES buffer.

  • HEPES, 3 mM SDS, pH 8 Come in convenient ready to use packs with no weighing or adjusting of pH required. These buffer packs have a longer shelf life when compared to the buffer solutions Highly reproducible better compositions Application(s): JAW™ Tris-HEPES-SDS Buffer

  • …the target cell with assistance from perforin.-á Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases.-á This induces apoptosis in the target cell. Physical form: Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

  • … Formula Weight: 238.30 CAS No.: 7365-45-9 Purity: ≥99.5% (titration) Protocols & Applications: Ensure correct concentrations of buffer solutions with the Buffer Calculator Other Notes: Easily compare specifications for HEPES products with the HEPES specification table .

  • MDL No.: MFCD07370436 Storage: -20C UNSPSC Code: 12352203 Immunogen: synthetic phosphopeptide corresponding to the region of human ATF2 containing serines 490 and 498. Physical form: Solution in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 mug/mL BSA, and 50% glycerol.

  • …(IAP) protein family, inhibits the activation of caspase 9 in cell extracts incubated with cytochrome c and dATP. Physical form: Solution in 25 mM Hepes, pH 7.5, 0.1 M sodium chloride, and 1 mM dithiothreitol. Analysis Note: Biological activity is measured by its ability to inhibit DEVD-afc…

  • …blotting (diluted 1:1000). Immunogen: synthetic phosphopeptide corresponding to amino acids residues surrounding the Xenopus polo-like kinase kinase phosphorylated on serines 482, 486 and 490. Physical form: Solution in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 mug/mL BSA, and 50% glycerol.

  • Grade: Biotechnology Molecular Weight: 238.31

  • … Immunogen: synthetic phospho-Tyr1054/1055 peptide corresponding to residues around Tyr1054/1055 of human Tyk2. Physical form: Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 mug/ml bovine serum albumin and 50% glycerol Specificity: Detects human Tyk2 only when…

  • …outer mitochondrial membrane where its interaction with Bak alters mitochondrial membrane permeability. Physical form: 0.2 mum filtered solution in 25 mM HEPES, pH 7.5, and 0.1 M KCl. Analysis Note: Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.

  • …lung. Immunogen: synthetic peptide corresponding to residues around Ser392 of human p53 conjugated to KLH. Physical form: Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 mug/ml bovine serum albumin and 50% glycerol Specificity: Does not react with…

  • …in tumorigenesis and may serve as a potential therapeutic target and biomarker. Immunogen: phosphopeptide corresponding to amino acid residues surrounding the pSer523 of human 5-lipoxgenase (5-LO). Physical form: Solution in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 mug/mL BSA, and 50% glycerol.

  • …it is also involved in a wide variety of physiological functions in tissues and organs outside the brain. Immunogen: synthetic peptide from the N-terminus of the rat GABAA receptor alpha4 subunit. Physical form: Solution in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 mug/mL BSA and 50% glycerol.

  • …inattentiveness. Immunogen: synthetic peptide from the extracellular loop 2 region of human dopamine transporter. Physical form: Solution in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 mug/mL BSA, and 50% glycerol. Target description: The dopamine transporter (DAT1) acts to take released dopamine…

  • …inattentiveness. Immunogen: synthetic peptide from the intracellular C-terminal region of human dopamine transporter. Physical form: Solution in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 mug/mL BSA, and 50% glycerol. Target description: The dopamine transporter (DAT1) acts to take released…

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