Egg Yolk Emulsion

for microbiology Storage: 2-8°C

Difco™ Egg Yolk Enrichment 50% is a concentrated egg yolk emulsion recommended for use in a variety of media such as Difco™ SFP Agar Base and Difco™ McClung Toabe Agar Base for the isolation and identification of Clostridium species on the basis of their lecithinase activity.

…Organisms producing lecithinase break down lecithin present in the egg yolk emulsion producing an insoluble opaque precipitate around the colonies. Lipase-producing organisms break down free fatty acids (in the egg yolk emulsion) forming an iridescent sheen on the surface of the colonies. Lipase…

CRITERION™ MYP Agar Base for the cultivation and enumeration of Bacillus cereus from foods. Antimicrobic Vial P, product #232681 and Egg Yolk Emulsion, product #215329, is required for this medium.

CRITERION™ Baird-Parker Agar Base is used with Egg Yolk Tellurite Enrichment as a selective medium for the detection and enumeration of coagulase-positive staphylococci from food samples. Egg Yolk Tellurite Emulsion, Cat. no. R450330, is required for this meduim.

…the formulation of Shahidi and Ferguson (1). Lecithinase and sulphite reactions can be identified on this medium. The medium along with the egg yolk emulsion and the supplement containing kanamycin and polymyxin B as the selective agents give high degree of selectivity for C. perfringens .Tryptose,…

…activity. Baird-Parker Agar Base can also be used to detect coagulase activity by adding plasma fibrinogen mixture in place of egg yolk emulsion. 375 mg bovine fibrinogen, 2.5 ml rabbit plasma, 2.5 mg trypsin inhibitor and 2.5 mg potassium tellurite dissolved in 10 ml sterile distilled…

…accompanying microflora and allow the growth of Listeria species. Soya lecithin has similar properties as that of egg yolk; hence additional supplementation of egg yolk emulsion is not required. After incubation at 30°C for 24-48 hours, approximately 0.1 ml of the broth is streaked on Listeria…

…Mannitol fermentation can be detected by phenol red, which yields yellow color to the mannitol fermenting colonies due to acid production. Added egg yolk emulsion helps in differentiation of lecithinase producing colonies, which are surrounded by a zone of white precipitate. Addition of Polymyxin B…

…an opaque zone due to coagulase activity within 24-48 hours incubation at 35°C. Reduction in tellurite is necessary because of absence of egg yolk emulsion. This results in translucent agar and white to grey coloured colonies of Staphylococci. For quantitative results select 20-200 colonies. Count…

…opaque zone due to coagulase activity within 24-48 hours incubation at 35°C. Reduction in tellurite is necessary because of absence of egg yolk emulsion. This results in translucent agar and white to grey coloured colonies of Staphylococci. For quantitative results select 20-200 colonies. Count…

…Selective Agar Base MOSSEL Description: Typical Composition (g/L):Peptone from meat 10.0; meat extract 1.0; D(-)mannitol 10,0; sodium chloride 10.0; phenol red 0.025; agar-agar 12.0. Also to be added: Egg-yolk emulsion 100mL, Cereus Selective Supplement (Cat. No. 1.09875.0001). Applicati