Dnase I Solution

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Dnase I Solution
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  • DNase I

    Bio Basic Inc.

    DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3"-OH oligonucleotides. Mg2+ I in the reaction solution causes the enzyme to produce nicks in doublestranded DNA, while in the presence of Mn2+, DNase I

  • illustra RNAspin 96 Kit

    GE Healthcare

    …high reproducibility in under 70 min. Integrated wash plate eliminates risk of contamination. Flexible processing by vacuum or centrifugation. DNase I treatment removes genomic DNA, leading to high-quality total RNA product. For fast and efficient purification in a 96-well format.96 samples can…

  • DNase I Kit, Amplification Grade

    MilliporeSigma

    …in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop Solution. Heating also denatures hairpins in the RNA, so the RNA can be used directly in reverse transcription. Many commercial DNase I formulations are contaminated with residual RNases.…

  • Deoxyribonuclease I from bovine pancreas lyophilized powder, Protein =85 %, =400…

    MilliporeSigma

    …after polyacrylamide gel electrophoresis. Preparation Note 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains…

  • Deoxyribonuclease I from Bovine Pancreas

    MP Biomedicals

    …by DNase I. The intracellular functions of the enzyme are probably controlled by a DNase inhibitor, which according to Lazarides and Lindberg is actin. DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. Deoxyribonuclease I from…

  • Deoxyribonuclease I from bovine pancreas Type II-S, lyophilized powder, Protein …

    MilliporeSigma

    …4.0-9.0, except phosphate buffer. Calcium chelators should be avoided. 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It…

  • DNase I

    IBI Scientific

    …for both in column DNase digestion and DNase digestion in solution. The IB47450 DNase I product will accommodate a 50-prep RNA extraction kit and consists of: 275 µl DNase I (2U/µl) and 2.5ml DNase I Reaction Buffer. The quality of the RNase-Free DNase I set is tested on a…

  • Magnesium Sulfate

    MP Biomedicals

    …integrity, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including deoxyribonuclease (DNase), the restriction enzymes EcoR I and EcoR V, and Ribonuclease H.Magnesium also stabilizes polymeric nucleic acids such as transfer RNA and ribozymes.

  • DNase I (bovine pancreas)

    bioWORLD

    DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3'-OH oligonucleotides. Mg+2 in the reaction solution causes the enzyme to produce nicks in double-stranded DNA, while in the presence on Mn+2, DNase I

  • SIGMA Deoxyribonuclease I bovine recombinant, expressed in Pichia pastoris,…

    MilliporeSigma

    Synonym(s): DNAse I; Deoxyribonucleate 5'-oligonucleotido-hydrolase MDL Number: MFCD00130918 Storage: −20°C Included Fees: C963P45 has a Dry/Wet Ice Fee included in the price. C963P46 has a Dry/Wet Ice Fee included in the price. To see the value of these fees, download our…

  • SIGMA Anti-CAD antibody produced in rabbit affinity isolated antibody, buffered…

    MilliporeSigma

    Synonym(s): Anti-Caspase Activated DNase I; Anti-DFF40; Anti-DNA Fragmented Factor 40 MDL Number: MFCD02262671 Storage: −20°C

  • rDNase Set

    MACHEREY-NAGEL

    Highly specific recombinant DNase for efficient removal of contaminating DNA On-column DNA digestion – for time saving DNA removal during RNA preparation DNA digestion in solution allows most efficient DNA removal for sensitive applications No RNase activity detectable RNA…

  • Ribonuclease A from bovine pancreas salt-free, lyophilized powder

    MilliporeSigma

    …this application, DNase free RNase A is used at a final concentration of 10 mg/mL. Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a…

  • HT 8-oxo-dG ELISA Kit II

    Trevigen

    …and saliva samples. KIT COMPONENTS: 8-OHdG Standard, 20 µm Assay Diluent 8-OHdG monoclonal antibody Goat anti-Mouse IgG-HRP Pre-coated 96-stripwell plate  100X Cation DNase I (5 Units/µl) Alkaline Phosphatase (1 Unit/µl) TACS-Sapphire

  • Ready One Step RT - PCR Kit

    Amresco

    …the need for Ethidium bromide. DNase I (Thomas No. C000V75) treated RNA (1µg) purified from 1x107 K562 cells with RiboZol (Thomas No. C999K56) was placed in a PCR tube.  Forward and Reverse Primers for GAPDH were added to the RNA template. The nucleic acid solution was heat-denatured at 65°C for…

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