Dnase I Solution

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Dnase I Solution
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  • DNase I

    IBI Scientific

    …for both in column DNase digestion and DNase digestion in solution. The IB47450 DNase I product will accommodate a 50-prep RNA extraction kit and consists of: 275 µl DNase I (2U/µl) and 2.5ml DNase I Reaction Buffer. The quality of the RNase-Free DNase I set is tested on a…

  • DNase I

    Bio Basic Inc.

    DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3"-OH oligonucleotides. Mg2+ I in the reaction solution causes the enzyme to produce nicks in doublestranded DNA, while in the presence of Mn2+, DNase I

  • DNase I (bovine pancreas)

    bioWORLD

    DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3'-OH oligonucleotides. Mg+2 in the reaction solution causes the enzyme to produce nicks in double-stranded DNA, while in the presence on Mn+2, DNase I

  • illustra RNAspin 96 Kit

    GE Healthcare

    …high reproducibility in under 70 min. Integrated wash plate eliminates risk of contamination. Flexible processing by vacuum or centrifugation. DNase I treatment removes genomic DNA, leading to high-quality total RNA product. For fast and efficient purification in a 96-well format.96 samples can…

  • DNase I, Amplification Grade

    MilliporeSigma

    …reaction buffer and Stop Solution for complete inactivation of DNase I General description: Deoxyribonuclease I (DNase I) is an endonuclease isolated from bovine pancreas that digests double and single stranded DNA into oligo and mononucleotides. Amplification Grade DNase I has been purified to…

  • Deoxyribonuclease I RNase-free solution from bovine pancreas,

    MilliporeSigma

    DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF. Unit Definition: One unit will cause a DeltaA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate. Other Notes: RNA treated with this DNase I should…

  • Deoxyribonuclease I from Bovine Pancreas

    MP Biomedicals

    …by DNase I. The intracellular functions of the enzyme are probably controlled by a DNase inhibitor, which according to Lazarides and Lindberg is actin. DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. Deoxyribonuclease I from…

  • DNA Polymerase I from Escherichia coli lysogenic for NM 964, buffered aqueous…

    MilliporeSigma

    …is replaced. Application: DNA Polymerase I from Escherichia coli has been used to study the effects of the anti-tumor drug cis-diaminedichloroplatinum (II) on the enzyme activity. Components: DNase Polymerase I is supplied in a solution of 50% glycerol containing 100 mM potassium phosphate…

  • Deoxyribonuclease I from bovine pancreas lyophilized powder, Protein =85 %, =400…

    MilliporeSigma

    …after polyacrylamide gel electrophoresis. Preparation Note 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains…

  • Deoxyribonuclease I from bovine pancreas Type II-S, lyophilized powder, Protein …

    MilliporeSigma

    …4.0-9.0, except phosphate buffer. Calcium chelators should be avoided. 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It…

  • Deoxyribonuclease I bovine, recombinant, expressed in Pichia pastoris, buffered…

    MilliporeSigma

    …activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved. Physical form: Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50%…

  • SIGMA Anti-CAD antibody produced in rabbit affinity isolated antibody, buffered…

    MilliporeSigma

    Synonym(s): Anti-Caspase Activated DNase I; Anti-DFF40; Anti-DNA Fragmented Factor 40 MDL Number: MFCD02262671 Storage: −20°C

  • Lambda Phage DNA, Non-methylated from Escherichia coli host strain GM119 (rm-…

    MilliporeSigma

    …is finally extracted by phenol-chloroform solution. Application: Lambda Phage DNA, Non-methylated from Escherichia coli host strain GM119 is suitable for use as a substrate for restriction enzymes. It was used as substrate for Prevotella ruminicola DNase activity studies. It was also used as a GC…

  • Anti-DNASE1 (91-105) antibody produced in rabbit, IgG fraction of antiserum,…

    MilliporeSigma

    …about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if. Biochem/physiol Actions: DNase I is an endonuclease that hydrolyzes double-stranded or single-stranded DNA preferentially at sites adjacent to pyrimidine nucleotides to produce a…

  • Anti-DNASE1L3 antibody produced in rabbit, affinity isolated antibody, buffered…

    MilliporeSigma

    …hypocomplementemic urticarial vasculitis syndrome (HUVS). Immunogen: Recombinant fragment corresponding to a region within amino acids 115 and 305 of DNase IL3 according to AAH15831 Physical form: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

  • Anti-DR3, Extracellular Domain antibody produced in rabbit, IgG fraction of…

    MilliporeSigma

    …chromosomal DNA by activating DNase. It also activates NF-κB, a protein complex that controls transcription of DNA. Immunogen: synthetic peptide corresponding to amino acids 59-77 of the extracellular domain of human DR3 precursor. Physical form: Solution in phosphate buffered saline…

  • Anti-DNASE1L3 antibody produced in rabbit, affinity isolated antibody, buffered…

    MilliporeSigma

    NOTE: Due to special handling or shipping requirements, these products will have additional fees added during checkout. Click HERE for a description of what fees might be charged.   Synonyms: DHP2; DNAS1L3; DNASE1L3; LSD Storage: -20C UNSPSC Code: 12352203 Features and…

  • rDNase Set

    MACHEREY-NAGEL

    Highly specific recombinant DNase for efficient removal of contaminating DNA On-column DNA digestion – for time saving DNA removal during RNA preparation DNA digestion in solution allows most efficient DNA removal for sensitive applications No RNase activity detectable RNA…

  • Biotin-Nick Translation Mix,

    MilliporeSigma

    I, DNase I, 0.25mM dATP, 0.25mM dCTP, 0.25mM dGTP, 0.17mM dTTP and 0.08mM Biotin-16-dUTP. Principle: The nick translation method is based on the ability of DNase I to introduce randomly distributed nicks into DNA at low enzyme concentrations in the presence of MgCl2. E. coli DNA Polymerase I

  • DIG-Nick Translation Mix,

    MilliporeSigma

    …nucleotides). Principle: The nick translation method is based on the ability of DNase I to introduce randomly distributed nicks into DNA at low enzyme concentrations in the presence of MgCl2.E. coli DNA Polymerase I synthesizes DNA complementary to the intact strand in a 5'->3' direction using…

  • Ribonuclease A from bovine pancreas salt-free, lyophilized powder

    MilliporeSigma

    …this application, DNase free RNase A is used at a final concentration of 10 mg/mL. Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a…

  • Magnesium Sulfate

    MP Biomedicals

    …integrity, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including deoxyribonuclease (DNase), the restriction enzymes EcoR I and EcoR V, and Ribonuclease H.Magnesium also stabilizes polymeric nucleic acids such as transfer RNA and ribozymes.

  • HT 8-oxo-dG ELISA Kit II

    Trevigen

    …and saliva samples. KIT COMPONENTS: 8-OHdG Standard, 20 µm Assay Diluent 8-OHdG monoclonal antibody Goat anti-Mouse IgG-HRP Pre-coated 96-stripwell plate  100X Cation DNase I (5 Units/µl) Alkaline Phosphatase (1 Unit/µl) TACS-Sapphire

  • Ready One Step RT - PCR Kit

    Amresco

    …the need for Ethidium bromide. DNase I (Thomas No. C000V75) treated RNA (1µg) purified from 1x107 K562 cells with RiboZol (Thomas No. C999K56) was placed in a PCR tube.  Forward and Reverse Primers for GAPDH were added to the RNA template. The nucleic acid solution was heat-denatured at 65°C for…

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