Dmso Cell Culture

…routinely used for cell culture. Replaces fetal bovine and other sera on an equivalent volume basis. Quality Tested at a concentration of 10% for the ability to stimulate a =100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells Tested…

…routinely used for cell culture. Replaces fetal bovine and other sera on an equivalent volume basis. Quality Tested at a concentration of 10% for the ability to stimulate a = 100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells Tested…

This unique formulation of cell culture freezing media is for cryopreservation of a broad spectrum of mammalian cells (see viability data). The reagents used in these products are certified to be virus and mycoplasma free. Contains 10% DMSO, calf & fetal bovine serum

Storage: -20C UNSPSC Code: 12352200 Application: Recommended for the cryopreservation of cells. Other Notes: Complete ready-to-use medium. Proprietary formulation with Minimum Essential Medium, dimethyl sulfoxide, calf serum and fetal bovine serum.

Storage: −20°C

Storage: -20C UNSPSC Code: 12352200 Application: Recommended for the cryopreservation of cells. Other Notes: Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.

…at -20C, and to avoid solution precipitation under freeze-thaw cycles.2.The suggested concentration of Spectinomycin (based on literature) in cell culture assays is 0.2-100mγ/mL. Therefore, it is recommended to dilute the ready made solution 1:1000-500,000. Synonyms: Actinospectacin;…

…long-term culture assays mainly for growth of pluripotent stem cells. Feeder layer cells support the target cells by releasing growth factors to the cell culture and detoxification of the medium. Moreover, feeder layer cells play a role in ECM proteins synthesis that controls cell culture growth. In…

CryoXL™ Cell Freezing Medium 1X w/ FBS and DMSO w/o Antibiotics. Used for hardy cell lines that are less susceptible to freezing damage.

…interphase and mitotic cells. Malignant cells may be more susceptible to the antimicrotubular effect of nocodazole than nonmalignant cells. Mammalian cells cultured in vitro can be treated with 0.04-10 mug/mL doses for cell synchronization experiments. Prolonged arrest of cells in mitosis due to…

…Number: MFCD12197280 Purity: >=99% (HPLC) Storage: 2-8C Other Notes: Ganciclovir ready made solution in DMSO is provided at 10mM concentration (2.55mg/mL). It is suggested to dilute the provided ready made solution to a working concentration of 2-100muM for cell culture applications.

…1 µM against all four serotypes of DENV. The IC50 (µM) of Narasin against four different DENV serotypes was 0.05-0.65 µM. Other Notes: Narasin solution is provided at 1.307 mM (1 mg/ml). Typical final concentration for use in cell culture is 0.01-5 muM. Stable at -20 C for at least 2 years.

Synonyms: DMSO; Methyl sulfoxide Formula: (CH₃)₂SO Formula Weight: 78.13 CAS No.: 67-68-5 Purity: ≥99.5% (GC) Density: 1.10 g/mL(lit.) Caution: Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming…

…used in studies and suppression of HIV-replication. It has also been shown to suppress programmed cell death of PC12 cells induced by etoposide, an inhibitor of topoisomerase II. In cell culture, actinomycin D is used as a selection agent. Actinomycin D binding to yeast in ribosomal RNA has been…

Filipin Complex, the collective name given to family of isomeric polyene macrolides isolated from cultures of S. filipinensis, with Filipin III being the majority part of the complex. Purity: >=90% (HPLC) Storage: -20C Application: Filipin has been used in a double staining procedure as…

…to suppress the replication of hepatitis C virus genome in cultured hepatocytes. At concentrations >10 nM, CsA protected isolated hepatocytes against the action of phalloidin. CsA can inhibit IL2 production resulting from T cell activation via Calcineurin inhibition.An extensive list of references…

Filipin III is a polyene macrolide antibiotic and the major component isolated from cultures of S. filipinensis. 1 Due to its ability to fluoresce and bind to cholesterol, Filipin has been widelyused as a probe for sterol location in biological membranes 2 and has been used clinically as a stain for…

…RNA, and other biomolecules. These dyes are small, pH insensitive (between pH 3 and pH 10), and soluble in aqueous solution and have tolerance to DMSO. They are more photo-stable than fluorescein, have high molar extinction coefficients, and favorable quantum yields. Due to their physical and…

This unique formulation of cell culture freezing media is for cryopreservation of a broad spectrum of mammalian cells (see viability data). Freezing Media contain 10% of the active cryopreservation ingredient (either DMSO or Glycerol). The remaining 90% is a proprietary mixture of Calf and Fetal…

…The DMSO must be removed by centrifugation of the cells at resuscitation. 5% CO2; 37C. Other Notes: Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line.…

…as a supplement to tissue culture medium for the prevention of secreted proteins degradation. The product has been found to be non-toxic after a 48 hours exposure to the following adherent cell lines: A431, CHO, COS, HepG2, and HeLa, and to following non-adherent cell lines: Jurkat and HL-60. This…

…media: 10% DMSO. At confluence 5-7 x 104 cells/cm2 can be expected. Other Notes: Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have…

Cell Cryopreservation Medium with 10% DMSO is a balanced, protein free, ready-to-use cryopreservation solution with 10% USP DMSO (Cryopres™). It does not require any dilution or further processing and can be used for cells cultured in serum free medium or with serum containing medium.…

…cell and tissue culture, sample storage, and more. They are available with 0.5mL, 1.1mL (1.0mL capped), and 2.2mL (2.0mL capped) well volumes. Manufactured from high-quality virgin polypropylene, these plates are resistant to a wide variety of chemicals, including phenols, chloroform, and DMSO. They…

…tranfection of cells cell fusion procedures with 40-50% PEG mass spectrometry analysis of protein phosphorylation cryopreservation of cells Features and Benefits free from DNases, RNases, phosphatases and proteases tested for cell culture General description DMSO (dimethyl…

BRAND polystyrene and polypropylene microtitration plates are excellent for cell and tissue culture, sample storage, immunological assays, and other applications. Plates have 96 wells and are available with 330µL or 400µL well volumes. They are fully compatible with most standard plate readers…

…without Dimethyl Sulfoxide (DMSO) for the short-term storage of cells or tissue. For short-term storage, add cold (2-8 °C) 2-8 CELLsium at a recommended cell concentration of 1 x 10 6 mL to 8 x 10 6 mL. The 2-8 CELLsium can be removed after centrifugation, cell concentration adjusted and…

Dimethylsulfoxide (DMSO) is a very polar organic compound which used for solubilizing organic and inorganic compounds. In addition, DMSO is used in cell culture as a cryoprotectant added to cell culture medium, to preventformation of ice crystals during freezing of cells. DMSO can be used in…

…to the standard use of DMSO for cryopreservation of cell cultures. For more information see here. Application: CryoSOfree is a chemically defined DMSO-free cryopreservation medium. It is animal component free and has been shown to preserve the potential of stem cell for differentiation. …

…production. The cells are intended to be cultured in, and can be directly thawed into EX-CELL(R) CD HEK293 Viral Vector Medium.VirusExpress 293T Lentiviral Production Cells are derived from the HEK293T cell line (ATCC CRL-3216) through single cell cloning. The parental HEK293 cell line was…

…on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L. Biochem/physiol Actions: Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation…

…of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal-induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines, embryonic stem cells (ESC), and hematopoietic stem cells. DMSO…