Dispase Ii

Synonym(s): Protease from Bacillus polymyxa MDL Number: MFCD02253017 Activity: ≥0.5 units/mg solid Storage: 2-8°C

…EDTA, EGTA, Hg 2+ , other heavy metals. Dispase is not inhibited by serum. Working concentration: 0.6 to 2.4 U/ml Working solution: Preparation of stock and working solutions: To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase ® II enzyme in HEPES-buffered saline (50…

Dipase is an extremely stable microbial metalloprotease suitable for use in cell cultures. Dispase is suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. Dissolve the lyophilized enzyme in sterile water to 5 mg/mL. Further dilution with…

Synonym(s): Protease from Bacillus polymyxa MDL Number: MFCD02253017 Activity: ≥10 unit/mg solid Storage: 2-8°C

…should be frozen in aliquots. Avoid repeated freezing and thawing. The working solution diluted with PBS is stable at 2 to 8 °C for 3 days. Specificity: Dispase ® is a non-specific metalloprotease. Legal Information: Dispase is a registered trademark of Godo Shusei Co., Ltd.

Dispase, a bacillus-derived neutral metalloprotease that is recommended for recovering cells cultured on Corning® Matrigel®TM Basement Membrane Matrix. (100 ml). Molecular Weight: 36 kD Source: Bacillus polymyxa-derived metallo protease Quality: Tested for ability to solubilize a 1 mm…

…contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a high concentration of Dispase ® , a non-clostridial neutral protease. Liberase DL (Dispase Low) Research Grade is used for the dissociation of a…

…the enzyme blend as a result of higher Collagenase I + II purity (determined by HPLC analysis). • Obtain higher experimental reproducibility due to higher lot-to-lot consistency. Application: Liberase ™ DH (Dispase High) Research Grade is used for the dissociation of a broad…

…concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL) Enzyme activity: Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycar bonyl-Pro-Leu-Gly-Pro-D-A rg as the substrate) Dispase ® : 0.8 Wünsch U/mg lyophilizate (+37°C, casein…