Dispase Ii

…EDTA, EGTA, Hg 2+ , other heavy metals. Dispase is not inhibited by serum. Working concentration: 0.6 to 2.4 U/ml Working solution: Preparation of stock and working solutions: To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase ® II enzyme in HEPES-buffered saline (50…

…culture and passage of human embryonic and induced pluripotent stem cells.Dispase-II enzyme is produced in Bacillus polymyxa. Synonyms: Dispase, Dispase 2 Application: Research Sub CategoryEnzymes & Biochemistry Legal Information: PLURISTEM is a registered trademark of Merck KGaA,…

…concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL) Enzyme activity: Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycar bonyl-Pro-Leu-Gly-Pro-D-A rg as the substrate) Dispase ® : 0.8 Wünsch U/mg lyophilizate (+37°C, casein…

Synonym(s): Protease from Bacillus polymyxa MDL Number: MFCD02253017 Activity: ≥10 unit/mg solid Storage: 2-8°C

Synonym(s): Protease from Bacillus polymyxa MDL Number: MFCD02253017 Activity: ≥0.5 units/mg solid Storage: 2-8°C

…from Clostridium histolyticum and Bacillus polymyxa cultures devoid of animal containing reagents. This product is manufactured in an ISO9001:2008 certified enviroment. Synonyms: MMP, Matrix metalloprotease, Dispase, Neutral Protease Application: Research Sub CategoryEnzymes & Biochemistry

Dipase is an extremely stable microbial metalloprotease suitable for use in cell cultures. Dispase is suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. Dissolve the lyophilized enzyme in sterile water to 5 mg/mL. Further dilution with…

…should be frozen in aliquots. Avoid repeated freezing and thawing. The working solution diluted with PBS is stable at 2 to 8 °C for 3 days. Specificity: Dispase ® is a non-specific metalloprotease. Legal Information: Dispase is a registered trademark of Godo Shusei Co., Ltd.

…the enzyme blend as a result of higher Collagenase I + II purity (determined by HPLC analysis). • Obtain higher experimental reproducibility due to higher lot-to-lot consistency. Application: Liberase ™ DH (Dispase High) Research Grade is used for the dissociation of a broad…