Dispase Ii

Dispase, a bacillus-derived neutral metalloprotease that is recommended for recovering cells cultured on Corning® Matrigel®TM Basement Membrane Matrix. (100 ml). Molecular Weight: 36 kD Source: Bacillus polymyxa-derived metallo protease Quality: Tested for ability to solubilize a 1 mm…

…be added to the gel prior to plating at a recommended density of 3-4 × 10 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml. Other Notes: ECM gel is composed primarily…

Synonym(s): Protease from Bacillus polymyxa MDL Number: MFCD02253017 Activity: ≥0.5 units/mg solid Storage: 2-8°C

Dipase is an extremely stable microbial metalloprotease suitable for use in cell cultures. Dispase is suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. Dissolve the lyophilized enzyme in sterile water to 5 mg/mL. Further dilution with…

Synonym(s): Protease from Bacillus polymyxa MDL Number: MFCD02253017 Activity: ≥10 unit/mg solid Storage: 2-8°C

…be added to the gel prior to plating at a recommended density of 3-4 × 10 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml. Caution: ECM gel may be stored up to 72…