Defibrinated Blood

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Defibrinated Blood
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  • Sheep Blood, Defibrinated

    Hardy Diagnostics

    Our animal blood is obtained from a controlled herd that is kept free of antimicrobic drugs and disease. All animals are maintained in a healthy condition and treated humanely in accordance with government regulations. Fresh blood is delivered to Hardy Diagnostics every week. We ship to our…

  • Vial Sheep Blood, Defibrinated

    BD

  • Blood Agar Base No. 2

    HiMedia

    …animal blood or type of base medium used (6). Directions: Suspend 21.25 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 40 - 50°C and aseptically add 7% v/vsterile defibrinated blood.

  • Tryptose Agar

    HiMedia

    blood (sheep, horse) to prepare blood agar for the isolation of fastidious organisms like Brucella. Dextrose is the source of energy. Tryptose serves as nitrogen source while sodium chloride maintains osmotic equilibrium. Blood Agar may be prepared by adding 5%v/v sterile defibrinated blood to…

  • Horse Blood, Defibrinated

    Hardy Diagnostics

    Our animal blood is obtained from a controlled herd that is kept free of antimicrobic drugs and disease. All animals are maintained in a healthy condition and treated humanely in accordance with government regulations. Fresh blood is delivered to Hardy Diagnostics every week. We ship to our…

  • Dextrose Agar

    HiMedia

    Dextrose in culture media serves as a source of energy. A basal media with 0.5 - 1.0% dextrose, supplemented with defibrinated blood is recommended for the isolation of a wide variety of fastidious organisms (1). Dextrose Agar, recommended by APHA (2), contains 1.0% dextrose and therefore supports…

  • Heart Infusion Broth, HiVeg™

    HiMedia

    …grams of MV169 or 25.0 grams of MV170 in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired 5% sterile defibrinated blood may be added. Mix well and pour into sterile petri plates or tubes.

  • Clostridium Difficile Agar

    bioWORLD

    …Clostridium Difficile selective Supplement (see below), previously reconstituted in 10 ml of sterile distilled water and 70ml sterile defibrinated horse blood. Mix thoroughly but gently to avoid bubble formation and dispense into Petri dishes or sterile tubes. Clostridium difficile selective…

  • Brain Heart Infusion Agar (Special Infusion Agar)

    HiMedia

    …of 50 mg/l chloramphenicol or 40mg/l streptomycin or a mixture of 50mg/l gentamicin and 50mg/l chloramphenicol along with 5-10% sterile defibrinated blood is often recommended for inhibition of bacteria and isolation of pathogenic systemic fungi. A mixture of cycloheximide (0.5 g/l) and…

  • Trypto Soy Agar

    RPI (Research Products International)

    …growth medium prepared with or without blood or enrichments. It is used for the isolation of different strains of fastidious microorganisms. A blood agar medium may be prepared by adding 5 to 10% sterile defibrinated blood. TSA with 5% sheep blood is useful for the isolation and determination…

  • Nutrient Agar 1.5%

    HiMedia

    …organic nitrogen compounds and salts. Nutrient Agar 1.5% may be used for blood culturing work after the addition of sterile 5-10% v/v defibrinated blood. Sodium chloride makes the medium isotonic preventing haemolysis of red blood corpuscles. Directions: Suspend 31 grams in 1000 ml distilled…

  • Columbia CNA Agar

    RPI (Research Products International)

    …stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes. Prepare 5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45-50°C. Dispense into petri dishes or tubes.

  • Nutrient Agar 1.5%

    HiMedia

    …and salts. Sodium chloride makes the medium isotonic preventing haemolysis of red blood corpuscles. This Nutrient Agar may be used for blood culturing work after the addition of sterile 5% v/v defibrinated blood and additional Sodium chloride (3g/l) (1). Storage and Shelf-life: Store below…

  • Lymphosep™-Lymphocyte Separation Medium

    MP Biomedicals

    …a clear separation of lymphocytes from heparinized or defibrinated human blood. Ready to use quick, simple and reliable medium for the isolation of lymphocytes from whole blood. Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium metrizoate that contains a…

  • Brain Heart Infusion Agar, HiVeg™ (Special Infusion Agar, HiVeg™)

    HiMedia

    blood, Gentamicin and Chloramphenicol (4, 5, 6). HiVeg special infusion, HiVeg infusion, HiVeg peptone No.3 and dextrose provides source of nitrogen, carbon, vitamins and chloride maintains osmotic equilibrium. Phosphate provide good buffering action in these media. When defibrinated sheep blood

  • LSM™ - Lymphocyte Separation Medium

    MP Biomedicals

    …solution of a sucrose polymer and diatrizoate salts. LSM™ is formulated for isolation of mononuclear cells from defibrinated or heparinized whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most…

  • Casein Soyabean Digest Agar (Soyabean Casein Digest Agar) (Tryptone Soya Agar)

    HiMedia

    …1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired, aseptically add 5% v/v defibrinated blood in previously cooled medium to 45-50°C for cultivation. Mix well and pour into sterile Petri plates.

  • Tryptose Phosphate Broth

    HiMedia

    …growth and aids in dispersion of reducing substances and carbon dioxide formed in the environment (2). For blood culture work aseptically add 10 ml of sterile defibrinated blood to 150 ml of sterile medium in 300 ml Erlenmeyer flask. Incubate and subculture on other media. Storage and…

  • Brain Heart Infusion Broth

    HiMedia

    …(2-4). This medium is nutritious and well buffered to support the growth of wide variety of organisms (2, 5, 6). With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum (7) and other fungi. For selective isolation of fungi, addition of…

  • Brucella Agar Base, Modified

    HiMedia

    …but mainly it serves as a source of Vitamin B complex. Dextrose serves as an energy source. It can be enriched with 5% v/v sterile defibrinated horse blood. For selective isolation of Brucella species, antibiotic mixtures are incorporated into the base (2,3,4). Farrel and Robinson formulated…

  • Brucella Agar Base

    HiMedia

    …maintain the osmotic equilibrium of the medium. Dextrose serves as an energy source. The medium can also be enriched with 5 % v/v sterile defibrinated horse blood. For selective isolation of Brucella species antibiotic mixtures in the form of freeze dried supplements (FD) are incorporated into the…

  • Anaerobic CNA Agar Base

    HiMedia

    …exhibited luxuriant growth of fastidious organisms like Streptococci, Enterococci, and Staphylococci etc. on supplementation with sterile defibrinated sheep blood. Anaerobic CNA Agar Base is a modification of Columbia CNA Agar base with additional enrichment supplements i.e. vitamin K1 and hemin…

  • Brain Heart Infusion Broth with 0.1% Agar

    HiMedia

    …(2-4). This medium is nutritious and well buffered to support the growth of wide variety of organisms (2, 5, 6). With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum (7) and other fungi. Agar in 0.1% concentration improves growth…

  • Bovine Thrombin, Low Specific Activity Diagnostic Use

    MP Biomedicals

    Thrombin is used throughout the diagnostics industry in a variety of coagulation assays, clotting factor tests and defibrination of blood or plasma for serum controls. Thrombin is also used for site specific cleavage of recombinant fusion proteins, and in biochemical and medical research…

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