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Hamilton

HC-75 Cation Exchange HPLC Column

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HC-75 Cation Exchange HPLC Column

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Polymeric 7.5% cross-linked soft-gel columns for cation, ligand exchange separations of carbohydrates.

  • Compatible with UV and RI detectors
  • Hydrogen form columns for USP L17 applications
  • Calcium form columns for USP L19 applications
  • Lead form columns for USP L34 applications
  • One particle size: 9 μm
  • Three column internal diameters: 4.1, 7.8 and 10.0 mm
  • One column material: 316 stainless steel
  • Three forms: calcium, lead and hydrogen

Separate compounds through ligand exchange.  HC-75 columns separate compounds through ligand exchange. The 8% cross-linked HC-75 uses ligand exchange as the primary mechanism of separation. The different forms of the HC-75 (Hydrogen, Calcium and Lead) each provide a unique selectivity for separating varying types of charged analytes based on electronegativity toward the counterion. The higher carbohydrate oligomers elute first while the smaller di- and monosaccharaides elute later.

Pack Your Own Columns - Hamilton offers a range of bulk polymer resins for customers who prefer to pack their own column hardware. Chromatographic capacity and efficiency tests are conducted on both bulk and packed column resins to ensure product integrity. Samples from different lots can be purchased for method and process validation.

Protect Your Investment - Hamilton guard columns protect analytical, semi-prep and preparative HPLC columns. They also remove particulate contaminants and highly adsorptive compounds from samples, prolonging column life. The designs are modular and cartridge replacement is easy and tool-free.  Cartridge holders for analytical columns are available in stainless steel and PEEK materials. Semi-prep/preparative holders are available only in stainless steel. The design of the cartridge holders is optimized to reduce dead volume and prevent extra column band broadening. Cartridge holders are reusable indefinitely.

Cation
Exchange
Columns
Thomas No. 0470U71
HC-75 Calcium
305 X 7.8mm
Thomas No. 1216M11
HC-75 9µm CAL
250 x 4.1mm
Thomas No. 1216M12
HC-75 H+F 9µm
305 x 7.8mm
Stationary PhaseHC-75 (Ca2+)HC-75 (Ca2+)HC-75 (H+)
ModeCation ExchangeCation ExchangeCation Exchange
USP ClassificationUSP L19USP L19USP L17
Packing Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic AcidPSDVB/Sulfonic Acid
Particle Size9 µm9 µm9 µm
Pore SizeGel-TypeGel-TypeGel-Type
FormatColumnColumnColumn
Hardware Inner Diameter7.8 mm4.1 mm7.8 mm
Hardware Length305 mm250 mm305 mm
Hardware MaterialStainless SteelStainless SteelStainless Steel
Max. Pressure400 psi400 psi400 psi
Exchange Capacity5 meq/gm5 meq/gm5 meq/gm
Mobile Phase Limits100% Water 0-40% Acetonitrile100% Water 0-40% Acetonitrile100% Water 0-40% Acetonitrile
Buffer StrengthWaterWater0.0-0.5N
Temp Limits25-90°C25-90°C25-90°C
Restoration25-90°CFlush with 1% calcium chloride at 0.1 mL/min overnight.Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight.
Compound ClassificationCarbohydratesCarbohydratesCarbohydrates
UOM1 Each1 Each1 Each
Exchange Capacity/Column32 meq/column12 meq/column32 meq/column
Cation
Exchange
Columns
Thomas No. 1207L06
HC-75 (Pb2+) 9 µm
7.8 x 100 mm
Thomas No. 1207L07
HC-75 (Pb2+) 9 µm
7.8 x 305 mm
Thomas No. 1207L08
HC-75 (H+) 9 µm
4.1 x 250 mm
Stationary PhaseHC-75 (Pb2+)HC-75 (Pb2+)HC-75 (H+)
ModeCation ExchangeCation ExchangeCation Exchange
USP ClassificationUSP L34USP L17USP L17
Packing Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic AcidPSDVB/Sulfonic Acid
Particle Size9 µm9 µm9 µm
Pore SizeGel-TypeGel-TypeGel-Type
FormatColumnColumnColumn
Hardware Inner Diameter7.8 mm7.8 mm7.8 mm
Hardware Length100 mm305 mm250 mm
Hardware MaterialStainless SteelStainless SteelStainless Steel
Max. Pressure400 psi400 psi400 psi
Exchange Capacity5 meq/gm5 meq/gm5 meq/gm
Mobile Phase Limits100% Water 0-40% Acetonitrile100% Water 0-40% Acetonitrile100% Water 0-40% Acetonitrile
Buffer StrengthWaterWater0.0-0.5N
Temp Limits25-90°C25-90°C25-90°C
RestorationFlush with 1% lead nitrate at 0.1 mL/min overnight.Flush with 1% calcium chloride at 0.1 mL/min overnight.Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight.
Compound ClassificationCarbohydratesCarbohydratesCarbohydrates
UOM1 Each1 Each1 Each
Exchange Capacity/Column
Bulk ResinThomas No. 1177G24Thomas No. 1177G25Thomas No. 1177G26
Stationary PhaseHC-75 (Ca2+)HC-75 (H+)HC-75 (Pb2+)
ModeCation ExchangeCation ExchangeCation Exchange
USP ClassificationUSP L19USP L17USP L34
Particle Size9 µm9 µm9 µm
Pore SizeGel-TypeGel-TypeGel-Type
FormatBulk ResinBulk ResinBulk Resin
Exchange Capacity5 meq/gm5 meq/gm5 meq/gm
RestorationFlush with 1% calcium chloride at 0.1 mL/min overnight.Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight.Flush with 1% lead nitrate at 0.1 mL/min overnight.
UOMper gramper gramper gram
Semiprep/Preparative
Guard Column
Thomas No. 1177G27
(Semiprep/Preparative Guard Column, Hydrogen Form)
Thomas No. 1177G28
(Semiprep/Preparative Guard Column, Carbonate Form)
Stationary PhasePRP-X400PRP-X500
ModeCation ExchangeCation Exchange
Packing Material TypePSDVB/Sulfonic AcidPSDVB/Sulfonic Acid
Particle Size12-20 µm12-20 µm
FormatGuard ColumnGuard Column
Hardware Inner Diameter4.6 mm4.6 mm
Hardware Length33 mm33 mm
Guard Column SizeSemiprep/PreparativeSemiprep/Preparative
Hardware MaterialStainless SteelStainless Steel
Max. Pressure400 psi400 psi
Compound ClassificationCarbohydratesCarbohydrates
UOM1 Each1 Each

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Product Detail
Thomas No.
0470U71
Mfr. No.
79436
Description
Column HC-75 Calcium 305 X 7.8mm
list price/quantitytotal
$0.00
Thomas No.
1216M11
Mfr. No.
79431
Description
HC-75 9µm CAL 250 x 4.1mm
list price/quantitytotal
$0.00
Thomas No.
1216M12
Mfr. No.
79544
Description
Column HC-75 H+F 9µm 305 x 7.8mm
list price/quantitytotal
$0.00
Thomas No.
1207L06
Mfr. No.
79240
Description
HC-75 (Pb2+) 9 µm 7.8 x 100 mm
list price/quantitytotal
$0.00
Thomas No.
1207L07
Mfr. No.
79438
Description
HC-75 (Pb2+) 9 µm 7.8 x 305 mm
list price/quantitytotal
$0.00
Thomas No.
1207L08
Mfr. No.
79476
Description
HC-75 (H+) 9 µm 4.1 x 250 mm
list price/quantitytotal
$0.00
Thomas No.
1177G24
Mfr. No.
79709
Description
HC-75 (Ca 2+), 9 µm Bulk Resin
Ready to Ship
list price/quantitytotal
$0.00
Thomas No.
1177G25
Mfr. No.
79711
Description
HC-75 (H+), 9 µm Bulk Resin
list price/quantitytotal
$0.00
Thomas No.
1177G26
Mfr. No.
79712
Description
HC-75 (Pb 2+), 9 µm Bulk Resin
list price/quantitytotal
$0.00
Thomas No.
1177G27
Mfr. No.
79917
Description
Semiprep/Preparative Guard Column, Hydrogen Form
list price/quantitytotal
$0.00
Thomas No.
1177G28
Mfr. No.
79919
Description
Semiprep/Preparative Guard Column, Carbonate Form
list price/quantitytotal
$0.00
$0.00 (0 Items)

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