Cloning Cylinder

Cloning cylinders are used to surround a single cell to create homogeneous culture of cells rather than mixed population Flat end insures tight sealing with surface of culture dish

Glass cloning cylinder for cloning or isolating individual cells. Place cylinder into bottom of flask or Petri dish to isolate individual or small group of cells. Disinfectant chambers to be used as described in the 1992 J.A.O.A.C. Hard Surface Carrier Test.

Clone a single cell or group of cells by surrounding them with this PYREX® glass open-ended cloning cylinder. Dip the end of the cylinder into sterile silicone grease before pressing to the bottom of a culture dish to create an isolated well. Cylinders are autoclavable or can be dry heat…

These cloning cylinders facilitate the isolation of individual cell clones, each generated from a single cell, yielding a homogenous population of cells. To isolate a clone, a thin layer of grease is applied to the bottom edge of the cylinder and then inverted over the clone of choice. After adding…

…from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells…

…from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells…

Store at ambient temp. Application: Research CategoryCell Culture

…using Culture Cylinders with the Delta T, select the appropriate size cylinder for the protocol and place the cylinder into the center of the dish. Pipette the specimen into the cylinder. Then pipette media around the cylinder to a depth equal to the depth in the cylinder, thereby equalizing…

Serine/threonine-protein kinase PLK4, PLK-4, procentriole formation, parental centriole cylinder, trophoblast differentiation, HAND1, MDFIC

Non-baffled flasks are ideal for growing, mixing and storing both aerobic and anaerobic cultures. Graduated to approximate volumes. Delong style neck is made to fit stainless steel or plastic closures, while reducing the amount of splashing that would reach the closure.