Centrifuge Cell Wash

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Centrifuge Cell Wash
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  • Nu-Serum™ IV Growth Medium Supplements

    Corning

    …routinely used for cell culture. Replaces fetal bovine and other sera on an equivalent volume basis. Quality Tested at a concentration of 10% for the ability to stimulate a =100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells Tested…

  • Nu-Serum™ Growth Medium Supplements

    Corning

    …routinely used for cell culture. Replaces fetal bovine and other sera on an equivalent volume basis. Quality Tested at a concentration of 10% for the ability to stimulate a = 100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells Tested…

  • Inclusion Body Wash Buffer

    Bio Basic Inc.

    …RNA, DNA, Lipopolysaccharide. To prepare high purity inclusion bodies from lysed E.coli cells, cell debris is separated from inclusion bodies by centrifugation. The inclusion body wash buffer then washes cell pellet and removes RNA, DNA, Lipid, lipoprotein in the presence of denaturant and mild…

  • Hermle Z326 Series Universal Centrifuges

    Benchmark

    …60mm dishes. Centrifugation of chamber slides and dishes have multiple applications, including: Inducing contact of bacteria or viral particles with cells (usually for the purpose of infection and consequent imaging) Inducing contact between cultured cells and particles…

  • BD Sero-Fuge 2000 Centrifuges (Blood Bank)

    BD

    Designed specifically for blood cell washing procedures and for the separation of serum from cells On-board tachometer and solid state digital timer Locking cover with lid seal gasket prevents opening during operation Whisper-quiet operation Ergonomically designed exterior and…

  • Protein-A Red Separopore®(Agarose)4B

    bioWORLD

    …conical centrifuge tube. Disrupt cells by repeated aspiration through a 21 gauge needle and transfer to a 15 ml conical centrifuge tube. Wash cell culture plate with additional 1.0 ml ice cold RIPA buffer and combine with original extract. Pellet cellular debris by centrifugation at…

  • Protein-A Separopore® 4B

    bioWORLD

    …conical centrifuge tube. Disrupt cells by repeated aspiration through a 21 gauge needle and transfer to a 15 ml conical centrifuge tube. Wash cell culture plate with additional 1.0 ml ice cold RIPA buffer and combine with original extract. Pellet cellular debris by centrifugation at 10,000xg…

  • illustra RNAspin 96 Kit

    GE Healthcare

    …or suitable centrifuges. For fast and efficient purification in a 96-well format. 96 samples can be parallel-processed with high reproducibility in under 70 min. Integrated wash plate eliminates risk of contamination. Flexible processing by vacuum or centrifugation. DNase I treatment…

  • OncoQuick®

    Greiner Bio-One

    …and centrifugated. Apart from erythrocytes and granulocytes, the separation medium also allows the elimination of lymphocytes and mononuclear cells to a wide extent. The disseminated tumour cells are enriched in the interphase. After harvesting, the enriched cell fraction is washed. The tumour cells

  • Niacin Assay Medium

    HiMedia

    …AOAC (M367), to prepare the inoculum. Following an incubation at 35-37°C for 18-24 hours, the cells are centrifuged and washed thrice with 0.85% saline. The appropriate dilution of cells so obtained is used to inoculate tubes of Niacin Assay Medium (M040), containing increasing concentrations…

  • Activin A from mouse, recombinant, expressed in E. coli, >=95% (SDS-PAGE), >=95%…

    MilliporeSigma

    …development. Physical form: Recombinant mouse Activin A is lyophilized with 0.02% TFA. Reconstitution: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to…

  • illustra Blood genomicPrep Mini Flow Kit

    GE Healthcare

    cells High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse Increased ease of use with reduced pipetting volume changes, one centrifugation

  • ES-R1 from mouse,

    MilliporeSigma

    …ampoule of ES cells is thawed in 37 C water bath and the contents quickly transferred to a 15 ml centrifuge tube. KSR medium is added drop wise to 5 ml. Cells are centrifuged at 150 x g for 5 minutes. Cells are resuspended in 5 ml of KSR medium. The prepared feeder flask is washed once with PBS and…

  • Quick-DNA Urine Kit

    Zymo Research Corporation

    …for up to one month by adding the Urine Conditioning Buffer. When ready to extract the urine DNA, just add the Clearing Beads, vortex, and centrifuge to collect the precipitate. Following precipitation, chemical lysis and enzymatic digestion are used to extract DNA from the precipitate. The…

  • Protein A HP MultiTrap

    GE Healthcare

    …(classic protocol). Remember to order the collection plates for washing and elution of the enriched protein There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the…

  • DNA-pure Yeast Genomic Kit

    …utilizes an easy to follow protocol: 1) pellet cells; 2) resuspend cells and add lysis solution; 3) add protein solubilization solution after a 10 minute incubation at 70 C; 4) centrifuge and then isopropanol precipitate DNA from the supernatant; 5) wash pellet and resuspend in TE or water. …

  • illustra blood genomicPrep Mini Spin Kit

    GE Healthcare

    cells. High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing. Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse. Increased ease of use with reduced pipetting volume changes, one centrifugation

  • Quanta Maxi Kit

    mdi Membrane Technologies

    …Isolation; in just 30 minutes Very high binding capacity; Up to 1200 μg No gravitational waiting Simple filtration to remove cell debris; No centrifugation No precipitation required for concentration Higher yields with low culture volume Highly concentrated, ultrapure pDNA…

  • Quanta Midi Kit

    mdi Membrane Technologies

    …Midiprep for pDNA Isolation Very high binding capacity; up to 350μg No gravitational waiting Simple filtration to remove cell debris; No centrifugation No precipitation required for concentration Higher yields with low culture volume Highly concentrated, ultrapure pDNA isolation…

  • Quanta Giga Kit

    mdi Membrane Technologies

    …sensitive mammalian cells. These kits offer high yields from both low copy as well as high copy number plasmids. The innovative buffer system and filter device provides efficient binding of pDNA onto the spin column. Washing is done with the help of provided wash buffers in order to remove…

  • Quanta Mega Kit

    mdi Membrane Technologies

    …sensitive mammalian cells. These kits offer high yields from both low copy as well as high copy number plasmids. The innovative buffer system and filter device provides efficient binding of pDNA onto the spin column. Washing is done with the help of provided wash buffers in order to remove…

  • ROCHE M30 CytoDEATH

    MilliporeSigma

    …The antibody solutions should be free of precipitate. If necessary, centrifuge the solutions at high speed prior to use. Additional working solutions required for the immunofluorescence and flow cytometry procedure: Washing buffer: PBS containing 0.1% Tween 20 Incubation buffer: PBS containing…

  • AbraMag™ Carboxyl Magnetic Beads

    Abraxis

    …of antibodies, proteins, cells, or bacteria from serum, cell culture supernatants, ascites, or other sample types. The magnetic beads are incubated with a sample containing the target and then held by a magnet next to the tube. After the unbound contaminants are washed from the beads, the bound…

  • AbraMag™ Amine Magnetic Beads

    Abraxis

    …of antibodies, proteins, cells, or bacteria from serum, cell culture supernatants, ascites, or other sample types. The magnetic beads are incubated with a sample containing the target and then held by a magnet next to the tube. After the unbound contaminants are washed from the beads, the bound…

  • ROCHE Genopure Plasmid Midi Kit

    MilliporeSigma

    …are partially lysed, allowing the plasmid DNA to escape the cell wall into the supernatant. The larger E. coli chromosomal DNA is trapped in the cell wall. The lysate is cleared of cellular debris by filtration or centrifugation, and the plasmid DNA-containing fraction is loaded onto a…

  • ROCHE Genopure Plasmid Maxi Kit

    MilliporeSigma

    …are partially lysed, allowing the plasmid DNA to escape the cell wall into the supernatant. The larger E. coli chromosomal DNA is trapped in the cell wall. The lysate is cleared of cellular debris by filtration or centrifugation, and the plasmid DNA-containing fraction is loaded onto a…

  • ROCHE Apoptotic DNA-Ladder Kit

    MilliporeSigma

    …or cell lysis is accomplished by incubating the sample with the special Binding/Lysis Buffer. The sample is centrifuged through a column that contains glass fiber fleece. In the Binding/Lysis Buffer, nucleic acids quickly and preferentially bind to the surface of the glass fibers. The Washing Buffer…

  • ROCHE mRNA Isolation Kit for Blood/Bone Marrow

    MilliporeSigma

    …• Lyse red blood cells and stabilize total nucleic acids with RNA/DNA Stabilization Reagent for Blood/Bone Marrow (separate reagent, not contained in the kit). • Bind total nucleic acids to Magnetic Glass Particles and collect by centrifugation. • Wash the Magnetic…

  • HOOK™ GST Protein Spin Purification Kit (Bacteria)

    G-Biosciences

    …resin is transferred to a convenient spin column, where it is rapidly washed and the GST protein is eluted. Bacterial- PELB™ kit has been developed for the extraction of soluble proteins from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows…

  • Bacterial Genomic DNA Isolation Kit

    G-Biosciences

    cells are broken open using a buffer containing a detergent and then a protease treatment. When the bacteria cells burst, they release DNA, and the detergent and protease initiate the breakdown of contaminant proteins. Heat and high salt washes denature more protein contaminants. Using a centrifuge,

  • ROCHE High Pure Plasmid Isolation Kit

    MilliporeSigma

    …digestion as plasmid DNA isolated by CsCl density centrifugation. Components: • Suspension Buffer • RNase A, dry powder • Lysis Buffer • Binding Buffer • Wash Buffer I • Wash Buffer II • Elution Buffer • High…

  • FastDNA® SPIN Kit for Feces

    MP Biomedicals

    …Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic…

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