Cell Lysis Buffer

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Cell Lysis Buffer
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  • The denaturing Cell Lysis Buffer is suitable for cell lysis of the following proteins: (1) The insoluble antigen, such as cytoskeleton and membrane protein, hydrophobic protein. (2) The proteins, epitopes of which are hidden in secondary even higher structure and some proteins were subjected to…

  • The Non-denaturing Cell Lysis Buffer contains no-ion detergent, salt, EDTA. The Lysis buffer is able to lyse cells from adherent cultures, suspension cultures as well as animal tissues. The Lysis buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins. Protein extracted can…

  • White blood cell samples are collected from whole blood and Buffy coat after removing red blood cells and readily lysed in the lysis buffer PL015. The supernatant can be used for isolation of Genomic DNA and subsequent extractions in lysis buffer PL015 also produce crude protein fraction suitable…

  • Synonyms: RBC Lysis Buffer; lysis buffer; red blood cells Storage: 2-8°C UNSPSC Code: 23201100 RIDADR: NONH for all modes of transport Application: Used for both DNA and RNA isolation, the buffer is designed for the preferential lysis of red blood cells from human whole blood,…

  • The buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.

  • The buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.

  • Red Blood Cell (RBC) Lysis Buffer is suitable for use in treatment of leukocytes from fresh whole blood or use in hydridoma protocol to remove red blood cells from mouse spleencyte suspensions before fusion. RBC lysis buffer selectively lyses the erythrocytes and leaves the leukocytes intact. The…

  • Animal Cells Protein Lysis Buffer, 1x Strength Solution: The Animal Cells Protein Lysis Buffer extracts cytoplasmic and nuclear protein from cultured mammalian cells. The buffer utilizes mild non-ionic detergent with our proprietary combination of various salts and reagents to enhance protein…

  • The IP/Western Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation. The Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This Ready-made formula is effective for extracting cytoplasmic, membrane and…

  • Lysis buffer for extracting proteins from cells. 1X concentration Tris-HCL, pH 7.4 (50mM) SDS (2%)

  • Lysis buffer for extracting proteins from cells. 1X concentration Tris-HCl, pH 7.4 (50mM) NaCl (150mM) Triton X-100 (1%) EDTA (5mM)

  • RBC Lysis Buffer

    IBI Scientific

    …such as cultured cells, tissue, bacteria and fungus. RBC Lysis Buffer is included with the kit to remove non-nucleated red blood cells and to reduce hemoglobin contamination. When the blood sample is less than 50µl or when using nucleated red blood cell samples, the Cultured Cells Protocol is …

  • RBC Lysis Buffer

    G-Biosciences

    Our RBC Lysis Buffer is designed for the optimal lysis of erythrocytes, while having minimal effect of lymphocytes, when used as directed. The use of RBC Lysis Buffer allows for the preferential lysis of red blood cells from whole blood and as these are the majority of cells in whole blood…

  • 10X RIPA Lysis Buffer

    Rockland Immunochemicals Inc

    … 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and biological activity. We recommend using 1.0 ml of RIPA Lysis Buffer to lyse 0.5…

  • Triton Lysis Buffer Solution

    RPI (Research Products International)

    1X buffer used to lyse cells under non-denaturing conditions. 1X Formulation: TRIS HCI: (pH 7.4) 50 mM NaCI: 150 mM TritonX-100: 1% EDTA: 5 mM

  • Sample buffer for HIS-TAG protein purification from E.coli and insect cells. pH 8.0 Na2HPO4 (50mM) NaCl (300mM) Imidazol (10 mL)

  • Bacteria Lysis Kit

    IBI Scientific

    …and Bacterial Lysis Buffer for initial lysing of the bacterial cell walls The Bacteria Lysis Kit is used with the IBI Tri-Isolate Kit when extracting RNA from bacteria samples. Bacterial cell walls are initially lysed by lysozyme, followed by the bacterial lysis buffer. The sample is…

  • A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification…

  • Extracts DNA from sperm cells, semen samples, and vaginal swabs. Produces single-stranded DNA validated for use with STR, PCR, and qPCR. Each extraction kit contains forensic GEM Sperm, Acrosolv , and ORANGE+ buffer. MicroGEM’s forensicGEM™ Sperm lysis kit uses a novel…

  • …acid. Configure the buffer to detect miRNA in cell lysates with a simple protocol modification. Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes…

  • …for cell input is provided The product ensures minimal RNA loss during preparation and analysis. The buffer employs a single-tube, solution-based format, rather than a column-based format. The process involves a single PBS wash followed by cell lysis in the SideStep lysis buffer

  • NP-40 Lysis High Salt Buffer Solution

    RPI (Research Products International)

    1X buffer used for extracting proteins from cells.

  • Chill to 2 to 8 °C prior to use. Lysis Buffer is provided as a ready-to-use 1X solution containing 50 mM Tris Cl, 150 mM Sodium Chloride, 1 mM EDTA, 1% TRITON-X-100, pH 7.4. No preparation is required prior to use.

  • …research tools and recombinant expression systems. The yeast cell is difficult to lysis, this is because yeast cells possess very complex proteinaceous cell wall that provides rigidity to the weak plasma membrane. Techniques for yeast cell lysis often involve harsh mechanical treatment while using…

  • …acetyltransferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase),immunoassays (e.g., Western blots, ELISAs, RIAs) and protein purifications. Tissue active protein extraction buffer is mild, contain minimal amount of detergent for cell lysis, and the detergent is dialyzable.

  • Bacterial Protein Lysis Buffer, 1x Stength Solution: Bacterial Protein Lysis Buffer enables mild extraction of proteins from bacteria (E. coli) without the need for mechanical disruption. The reagent may be used for soluble protein extraction and inclusion body purgation from bacterial cell lysates.…

  • RIPA Lysis Buffer is ready-to-use as a working 1X solution and requires no further dilution. RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein…

  • …Control cells are of a known concentration, containing the Internal Control DNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample DNA. The qPCR Extraction Control cells are spiked into the lysis buffer with the…

  • … 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and biological activity. We recommend using 1.0 ml of RIPA Lysis Buffer to lyse 0.5…

  • HOOK ™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK ™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB ™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western…

  • TNE Buffer

    Quality Biological

    TNE Buffer is often used as a base material to generate lysis buffers. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications More specifically, TNE Buffer is useful for: isolation of proteins from yeast cells as a lysis buffer, resuspension of pelleted virus or…

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