Cell Lysis Buffer

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Cell Lysis Buffer
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  • Nu-Serum™ IV Growth Medium Supplements

    Corning

    …routinely used for cell culture. Replaces fetal bovine and other sera on an equivalent volume basis. Quality Tested at a concentration of 10% for the ability to stimulate a =100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells Tested…

  • Nu-Serum™ Growth Medium Supplements

    Corning

    …routinely used for cell culture. Replaces fetal bovine and other sera on an equivalent volume basis. Quality Tested at a concentration of 10% for the ability to stimulate a = 100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells Tested…

  • ACK Lysing Buffer

    Quality Biological

    Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. Features Easy to use Fast Remove red blood cells while keeping the white blood cells Applications ACK Lysing Buffer is…

  • Lysis-EZ K (Red Blood Cell (RBC) Lysis Buffer)

    Bio Basic Inc.

    Red Blood Cell (RBC) Lysis Buffer is suitable for use in treatment of leukocytes from fresh whole blood or use in hydridoma protocol to remove red blood cells from mouse spleencyte suspensions before fusion. RBC lysis buffer selectively lyses the erythrocytes and leaves the leukocytes intact. The…

  • Animal Cells Protein Lysis Buffer

    Bio Basic Inc.

    The Animal Cells Protein Lysis Buffer extracts cytoplasmic and nuclear protein from cultured mammalian cells. The buffer utilizes mild non-ionic detergent with our proprietary combination of various salts and reagents to enhance protein extraction and stability. The buffer is compatible with …

  • Lysis-EZ H (IP/Western Lysis Buffer)

    Bio Basic Inc.

    The IP/Western Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation. The Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This Ready-made formula is effective for extracting cytoplasmic, membrane and…

  • Triton Lysis Buffer

    Lysis buffer for extracting proteins from cells. 1X concentration Tris-HCl, pH 7.4 (50mM) NaCl (150mM) Triton X-100 (1%) EDTA (5mM)

  • Tissue Proteins Lysis Buffer

    Bio Basic Inc.

    …protein kinase  assays (e.g., PKA, PKC, tyrosine kinase),immunoassays (e.g., Western blots, ELISAs, RIAs) and  protein purifications. Tissue active protein extraction buffer is mild, contain minimal amount of  detergent for cell lysis, and the detergent is dialyzable. 

  • MT Buffer

    MP Biomedicals

    MT Buffer is a lysis solution that is used as a stabilization agent during sample lysis. Carefully developed to protect and solubilize nucleic acids and proteins upon cell lysis. Allows extraction of genomic DNA with minimal RNA contamination. Found in the FastRNAPRO Soil DIrect and…

  • RBC Lysis Buffer

    IBI Scientific

    …such as cultured cells, tissue, bacteria and fungus. RBC Lysis Buffer is included with the kit to remove non-nucleated red blood cells and to reduce hemoglobin contamination. When the blood sample is less than 50µl or when using nucleated red blood cell samples, the Cultured Cells Protocol is …

  • SIGMA Red Blood Cell Lysing Buffer Hybri-Max™, liquid, sterile-filtered,…

    MilliporeSigma

    …to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is…

  • Hypotonic Lysis Buffer

    Amresco

    …well as protein assays, which are sensitive to the presence of detergents in the lysis buffer. Simply, resuspend the cell pellets in Hypotonic Lysis Buffer and incubate on ice for 15 minutes.  Disrupt the cells by homogenization and pellet the cellular debris.  The supernatant contains the…

  • Total Protein Cell Lysis Buffer

    Amresco

    Grade: Proteomics Easy-to-use lysis buffer for extraction of total cell protein. Total Cell Protein Lysis Buffer contains a non-ionic detergent as the lysis agent to reduce denaturation.

  • CelLytic™ NuCLEAR™ Extraction Kit For mammalian tissue or cultured cells

    MilliporeSigma

    …of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. CelLytic NuCLEAR offers the flexiblity you need for optimal…

  • Lysis-EZ G (Non-Denaturing Cell Lysis Buffer)

    Bio Basic Inc.

    The Non-denaturing Cell Lysis Buffer contains no-ion detergent, salt, EDTA. The Lysis buffer is able to lyse cells from adherent cultures, suspension cultures as well as animal tissues. The Lysis buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins. Protein extracted can…

  • Denaturing Lysis Buffer

    Lysis buffer for extracting proteins from cells. 1X concentration Tris-HCL, pH 7.4 (50mM) SDS (2%)

  • Protein pur-E.coli lysis Buffer

    Sample buffer for HIS-TAG protein purification from E.coli and insect cells. pH 8.0 Na2HPO4 (50mM) NaCl (300mM) Imidazol (10 mL)

  • 20% SDS

    Quality Biological

    … Common component of cell lysis buffers Filtered through a 0.22 µm membrane Dnase, Rnase, and Protease tested Applications This lauryl-grade sodium dodecyl sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods. Contents …

  • Genomic DNA Kits (Plant)

    IBI Scientific

    …chloroplast) from plant tissue and cells. Samples are disrupted both by grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and filtered to remove cell debris and salt precipitates. In the presence of the binding buffer coupled with chaotropic…

  • Lysis-EZ J (Denaturing Cell Lysis Buffer)

    Bio Basic Inc.

    The denaturing Cell Lysis Buffer is suitable for cell lysis of the following proteins: (1) The insoluble antigen, such as cytoskeleton and membrane protein, hydrophobic protein. (2) The proteins, epitopes of which are hidden in secondary even higher structure and some proteins were subjected to…

  • Lysis-EZ L (White Blood Cell Lysis Buffer)

    Bio Basic Inc.

    White blood cell samples are collected from whole blood and Buffy coat after removing red blood cells and readily lysed in the lysis buffer PL015. The supernatant can be used for isolation of Genomic DNA and subsequent extractions in lysis buffer PL015 also produce crude protein fraction suitable…

  • Bacteria Lysis Kit

    IBI Scientific

    …and Bacterial Lysis Buffer for initial lysing of the bacterial cell walls The Bacteria Lysis Kit is used with the IBI Tri-Isolate Kit when extracting RNA from bacteria samples. Bacterial cell walls are initially lysed by lysozyme, followed by the bacterial lysis buffer. The sample is…

  • TNE Buffer

    Quality Biological

    TNE Buffer is often used as a base material to generate lysis buffers. Filtered through a 0.22 µm membrane Dnase, Rnase, and Protease tested Applications More specifically, TNE Buffer is useful for: isolation of proteins from yeast cells as a lysis buffer, resuspension of…

  • Tris Protein Extraction Buffer (5x)

    Lysis buffer for extracting proteins from cells. 5X concentration Tris-HCL, pH 8.0 (0.5M) B-mercaptoethanol (100mM) EDTA (20mM) Add protease inhibitor according to your protocol

  • HEPES, Protein Extraction Buff(5X)

    bioWORLD

    HEPES Protein Extraction Buffer (5X) Lysis buffer for extracting proteins from cells.

  • TruePrime™ Single Cell WGA Kits

    Expedeon

    … TruePrime™ Single Cell WGA Kit version 2.0 uses a novel and reliable method to achieve accurate genome amplification from single cells. Dedicated buffers and enzymes deliver microgram quantities of DNA from a single cell or few cells. Up to 50 cells can be amplified with the same…

  • Yeast Protein Extraction Buffer Kit

    GE Healthcare

    cells. This kit eliminates the need for glass beads, a common method of mechanical lysis for yeast cells, and is a proprietary improvement on the Zymolyase based method for spheroplast preparation and extraction of soluble proteins from yeast cells. After cell lysis, Yeast Protein Extraction Buffer

  • 10% SDS

    Quality Biological

    … Common component of cell lysis buffers Filtered through a 0.22 µm membrane Dnase, Rnase, and Protease tested Applications This lauryl-grade sodium dodecyl sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods. Contents …

  • Trypsin-digestion Mix

    bioWORLD

    Lysis buffer for extracting proteins from cells. Used for efficient digestion of proteins with trypsin Modified Mass- Spec grade for use with the In-gel extraction kit Sufficient for 100 in gel digestions

  • 2-D Protein Extraction Buffer

    GE Healthcare

    cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. 2-D Protein Extraction Buffer offers a convenient way to prepare high-quality protein lysates. The buffers are designed to produce high spot resolution for 2-D gel analysis. Six buffers are…

  • RNASound™ RNA Extraction Cards

    FortiusBio

    …Extraction card enables high throughput and quick cell culture RNA extraction for gene transfection and expression screening. The plastic card holds eight 5mm filter paper discs that were treated with proprietary lysis buffer, cells are lysed upon applied on the card, RNA are released…

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