Corning® Nu-Serum™ IV Growth Medium Supplement is a low-protein alternative to newborn calf, fetal bovine, and other sera routinely used for cell culture.
Replaces fetal bovine and other sera on an equivalent volume basis.
Quality
- Tested at a concentration of 10% for the ability to stimulate a =100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells
- Tested for total protein content, pH, and osmolarity (information included with product)
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption
- Tested for endotoxin (LAL assay)
Formulation
Corning Nu-Serum™ IV - Frozen solution containing 25% fetal bovine serum, EGF, ECGS, insulin, human transferrin, triiodothyronine, progesterone, estradiol-17ß, testosterone, hydrocortisone, selenous acid, o-phosphorylethanolamine, glucose, amino acids, vitamins, and other trace elements and nutrients contained in the Ham's F12 medium base.
Storage
Shelf life: >= 3 months from date of shipment at -20°C