Blasticidin

Blasticidin S HCl is produced by Streptomyces griseochromogenes and inhibits translation in both prokaryotes and eukaryotes, thus preventing the formation of peptide bonds. Appearance: White powder Melting Point: 218.0–225.0°C (424.4-437.0°F) Water Solubility: Soluble

Blasticidin S is an antibiotic that also exhibits anti-parasitic activity. Blasticidin S inhibits protein synthesis by inducing deformations in P-site tRNA. Blasticidin S inhibits aflatoxin production in species of Aspergillus.

…plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase genes (bsr from Bacillus cereus or BSD from Aspergillus terreus), whose products convert blasticidin S to a non-toxic deaminohydroxy derivative. Blasticidin S belongs to the…

A peptidyl nucleaside antibiotic isolated from the culture broth of Streptomyces griseogenes , inhibits protein synthesis in both prokaryotes and eukaryotes.

Blasticidin S is used as a selection agent for transformed cells that contain the resistance genes bls, bsr, or BSD. Also used to study protein synthesis at the level of peptide bond formation. Soluble in water PubChem Chemical ID 16760315 Features CAS#: 3513-03-9 Molecular…

This product is a blasticidin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma's lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma's lenti-Cas9 plasmid is one part of a two-vector CRISPR system with…

Ready-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Blasticidin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Blast using…

A peptidyl nucleaside antibiotic isolated from the culture broth of Streptomyces griseogenes, inhibits protein synthesis in both prokaryotes and eukaryates. Supplied as a 10mg/mL sterile solution in HEPES buffer.

…Inhibits protein synthesis in prokaryotic and eukaryotic organisms. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin S inhibits hydrolysis of peptidyl-tRNA induced by release factors. It enhances the binding of tRNA to the large subunit of ribosomes…

…selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase gene (bsr) that converts blastisidin S to a nontoxic deaminohydroxy derivative. Synonyms: Blasticidin S, Hydrochloride, Streptomyces sp., Sterile-Filtered…

Formula: C17H26N8O5 HCl Formula weight: 458.90 Purity: 98+%, 10mg/ml in 1M HEPES buffer soln. CAS Number: 3513-03-9 UN Number: UN2810 Hazard Class: 6.1 Packing Group: III Harmonized Tariff Code:  2941.90 Hazard Statements: H302 Harmful if swallowed. Precautionary…

… Storage: -20C Biochem Physiol Actions: Blasticidin S belongs to the aminoacylnucleoside class of antibiotics. It inhibits protein synthesis in bacteria and eukaryotes. It also has fungicidal properties and prevents rice blast disease. Blasticidin S inhibits hydrolysis of peptidyl-tRNA induced…

Synonyms: Blasticidin S CAS Number: 589205 Formula: C17H26N8O5 · HCl Formula Weight: 458.9 Linear Formula: C17H26N8O5 · HCl MDL No.: MFCD02091640 Storage: -20C UNSPSC Code: 51101500 General description: Blasticidin S belongs to the aminoacylnucleoside class of antibiotics.…

This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of dCas9-VP64 and blasticidin resistance cassette linked by a 2A peptide (EF1a-dCas9-VP64-2A-Blasticidin) allowing for easy selection following successful transfection or transduction. Use Sigma’s…

…enable immediate transduction of a wide range of cell lines. dCas9-VP64 lentiviral particles efficiently and stably integrate dCas9-VP64 and blasticidin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-dCas9-VP64-2A-Blasticidin) for strong expression in both…

…driven by the EF1 alpha promoter (EF1a-dCas9-VP64-2A-Blasticidin). dCas9-VP64 lentiviral particles efficiently and stably integrate dCas9-VP64 and blasticidin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-dCas9-VP64-2A-Blasticidin). They are ideal for avoiding…

…human E1A-associated protein p300. The dCas9-p300 activator lenti plasmids use the EF1 alpha promoter for strong expression of dCas9-P300 and blasticidin linked by a 2A peptide (EF1a-dCas9-P300-2A-Blasticidin) allowing for easy selection following successful transfection or transduction. Use…

…by a 2A peptide (EF1a-MS2-P65-HSF1-2A-Hygromycin) and one plasmid that utilizes the EF1 alpha promoter to drive expression of dCas9-VP64 and blasticidin resistance cassette also linked by a 2A peptide (EF1a-dCas9-VP64-2A-Blasticidin). The vector composition allows for easy selection following…

…rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling. [1] The protein synthesis inhibitor blasticidin s enters mammalian cells via leucine-rich repeat-containing protein 8D. [2] Monoubiquitination of filamin b regulates vascular endothelial…

Microphthalmia-associated transcription factor (MITF) is a member of the basic/helix-loop-helix/leucine zipper (b-HLH-ZIP) transcription factor subfamily. MITF regulates the differentiation of melanocytes, optic cup-derived retinal pigment epithelium (RPE), and some types of bone marrow-derived…

…formulation. [Lennox formulation (0.5% sodium chloride) is often used to cultivate E. coli in the presence of salt-sensitive antibiotics (e.g., blasticidin, puromycin, and zeocin). Luria formulation (0.05% sodium chloride) can be used to optimize growth conditions by supplementing with additional…

Ready to use MS2-P65-HSF1 lentiviral particles enable immediate transduction of a wide range of cell lines. MS2-P65-HSF1 lentiviral particles efficiently and stably integrate MS2-P65-HSF1 and hygromycin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter…

…formulation. [Lennox formulation (0.5% sodium chloride) is often used to cultivate E. coli in the presences of salt-sensitive antibiotics (e.g., blasticidin, puromycin, and zeocin). Luria formulation (0.05% sodium chloride) can be used to optimize growth conditions by supplementing with additional…