B T Sure Incubator

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B T Sure Incubator
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  • …three step process Run indicator through standard sterilization cycle; compress plastic vial to break glass ampule inside and then incubate for a minimum of 48 hours. When complete, read color indicator: purple means effective sterilization; yellow and cloudy means ineffective sterilization.

  • incubated for 16 hours in 50μL SuRE/Cut Buffer B with an excess of Taq I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5μg [ 3 H] labeled calf thymus DNA are incubated

  • ROCHE Bcl I

    MilliporeSigma

    incubated for 16 hours in 50μL SuRE/Cut Buffer M with an excess of Bcl I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5μg [ 3 H] labeled calf thymus DNA are incubated

  • …is incubated for 16 hours in 50 μl SuRE/Cut Buffer L with an excess Rsa I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5 μg [ 3 H] labeled calf thymus DNA are incubated

  • incubated for 16 hours in 50μl SuRE/Cut Buffer B with an excess of Nru I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5μg [ 3 H] labeled calf thymus DNA are incubated

  • …recommend 10 U enzyme per ?g DNA and approximately 4 hour incubation time. Ligation and recutting assay Xba I fragments obtained by complete digestion of 1 ?g pUC18 DNA are ligated with 1 U T4 DNA Ligase in a volume of 10 ?l by incubation for 16 hours at +4°C in 66 mM Tris-HCl, 5 mM MgCl2,…

  • …is incubated for 16 hours in 50μl SuRE/Cut Buffer M with excess of Hind II. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5μg [3H] labeled calf thymus DNA are incubated

  • incubated for 16 hours in 50μl SuRE/Cut Buffer H with an excess of Afl III. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5μg [3H] labeled calf thymus DNA are incubated

  • incubated for 16 hours in 50 μl SuRE/Cut Buffer H with an excess of Sal I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5 μg [ 3 H] labeled calf thymus DNA are incubated

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