ESGRO Complete(TM) Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium (Cat. No. SF001-500). Accutase does not contain mammalian or bacterial derived products.
Application: Cell Detachment: 1. Thaw Accutase® to room temperature. 2. Wash plate, flask or beads with sterile PBS. 3. Add Accutase to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area. 4. Return culture to 37C incubator and allow cells to detach (5-10 minutes). 5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.
Legal Information: cOmplete is a trademark of Roche