10x Pcr Buffer

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10x Pcr Buffer
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  • Choice-Taq™ DNA Polymerase is a recombinant Taq DNA polymerase Choice-Taq™ is supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • Choice-Taq™ DNA Polymerase is a recombinant Taq DNA polymerase Choice-Taq™ is supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • …segments and 72 C to extend the DNA segments. Following electrophoresis of the reaction products in 1.5% agarose gel, a single band of approximately 500 base pairs was visualized for PCRs containing 1-1.5mM MgCl2. Application: 10x PCR Buffer without MgCl2 has been used with Sigma′s PCR enzymes.

  • Storage: −20°C

  • … The PCR buffer without MgCl2 is used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR). The buffer is ten-fold concentrated and should be used in combination with the MgCl2 Stock Solution. General description: The PCR buffer without…

  • …chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8). Application: Taq DNA Polymerase from Thermus aquaticus is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to…

  • MDL Number: MFCD00166026 Storage: −20°C

  • Storage: −20°C

  • …agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.The red dye has…

  • Concentration: 5u/µl Recombinant Taq DNA polymerase Supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • Concentration: 5u/µl Recombinant Taq DNA polymerase Supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • Concentration: 5u/µl Recombinant Taq DNA polymerase Supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • Storage: -20C UNSPSC Code: 41106300 Application: REDTaq(R) SuperPak(TM) DNA Polymerase is used for PCR DNA amplification methods. It contains the high visibility REDTaq(R) Genomic DNA polymerase and other reagents needed for PCR reactions.

  • 10x Taq Buffer

    Bio Basic Inc.

  • 10x Taq Buffer (MgCl2)

    Bio Basic Inc.

  • 10x Taq Buffer (NH4)2SO4

    Bio Basic Inc.

  • NH4 Buffer

    Bioline

    10x (NH4)2SO4 buffer for PCR Optimized - to improve PCR amplification rates Reproducibility - helps provide consistent results Product Description NH4 Buffer is an optimized, ready-to-use 10x buffer for use with DNA polymerases in PCR

  • ROCHE PCR Buffer Set

    MilliporeSigma

    …Solution 25 mM; PCR Buffer without Mg 2+ 10x concentrated; Alkaline Phosphatase Substrate, CSPD; Enhancer, Emerald II; Assay Buffer RIDADR: NONH for all modes of transport Application: For optimization of the magnesium-chloride concentration in the polymerase chain reaction (PCR) using Taq…

  • ROCHE PCR Core KitPLUS

    MilliporeSigma

    …Solution (10 mM of each dNTP); PCR Buffer with MgCl2 10x concentrated; PCR Buffer without MgCl2 10x concentrated; MgCl2 Stock Solution 25 mM RIDADR: NONH for all modes of transport Features and Benefits: The PCR Core KitPLUS is from Thermus…

  • PCR Reaction Buffer, without MgCl2, 100 mM Tris/HCl, 500 mM KCl, pH 8.3 (20 °C) 10x concentrated; MgCl2 Stock Solution 25 mM; PCR Buffer, 100 mM Tris/HCl, 15 mM MgCl2, 500 mM KCl, pH 8.3 (20 °C) 10x concentrated; Taq DNA Polymerase, in storage buffer:

  • … Components: Lysis Reagent; Neutralization Reagent; PCR Mix ready-to-use; Denaturation Reagent; Hybridization Buffer; Biotinylated Capture Probe; Washing Buffer 10x concentrated; Anti-Digoxigenin-POD antibody; Conjugate Dilution Buffer; TMB Substrate Solution; Stop Reagent; Positive Control…

  • …Code: 41106000 Components: PCR Fluorescein Labeling Mix, containing 2 mM dATP, dCTP, dGTP, dTTP, 0.5 mM Fluorescein-12-dUTP in water, pH 7.0 10x concentrated; PCR Reaction Buffer, without MgCl2, 100 mM Tris-HCl, 500 mM KCl, pH 8.3 (20 °C) 10x concentrated; MgCl2

  • …High Fidelity PCR System is the product of choice for multiplex PCR. FastStart High Fidelity Enzyme Blend, (5 U/μl) in storage buffer FastStart High Fidelity Reaction Buffer, 10x concentrated, with 18 mM MgCl2 FastStart High Fidelity Reaction Buffer, 10x concentrated, without…

  • …low; Control Template, high; Water, nuclease-free; Denaturing Reagent; Hybridization Buffer T; Hybridization Buffer IS; Washing Buffer 10x concentrated; Anti-DIG-HRP antibody; Conjugate Dilution Buffer; TMB Substrate Solution; Stop Reagent; Microplate; Cover Foil RIDADR: UN 3316 9 Features…

  • …Preparation Note: Working solution: Solution 5: Washing buffer, 1x Dilute an appropriate volume of Washing buffer, 10x conc. with autoclaved double-dist. water (1:10) and mix thoroughly. Approx. 2.5 ml of the diluted Washing buffer are needed for one reaction. Solution 6: Anti-DIG peroxidase,…

  • …U/μl; PCR DIG Probe Synthesis Mix, containing dATP, dCTP, dGTP (2 mM each) 10x concentrated; PCR Buffer with MgCl2 10x concentrated; dNTP Stock Solution, containing dATP, dCTP, dGTP, dTTP (2 mM each), pH 7.0 10x concentrated; Control Template, plasmid DNA in Tris/EDTA buffer, pH 8.0.…

  • …Fidelity Enzyme Blend, in storage buffer 5 U/μl; FastStart High Fidelity Reaction Buffer, with 18 mM MgCl2 10x concentrated; FastStart High Fidelity Reaction Buffer, without MgCl2 10x concentrated; MgCl2 Stock Solution 25 mM; DMSO; PCR Nucleotide Mix RIDADR: …

  • …Expand 20 kb PLUS Reaction Buffer, with 27.5 mM MgCl2 10x concentrated; MgCl2 Stock Solution 25 mM; Human Genomic DNA; Human β-globin Control Primer forward, (HβG forward); Human β-globin Control Primer reverse, (HβG reverse); PCR Nucleotide Mix RIDADR: …

  • …range pcr; long template pcr Storage: −20°C UNSPSC Code: 12352200 Components: Expand Long Template Enzyme Mix; Expand Long Template Buffer 1, with 17.5 mM MgCl2 10x concentrated; Expand Long Template Buffer 2, with 27.5 mM MgCl2 10x concentrated;…

  • … T/A cloning can be applied. For a standard 50 μl PCR, we recommend using 5 U of the enzyme blend. Contents: • Expand 20 kb PLUS Enzyme Mix • Expand 20 kb PLUS Reaction Buffer, 10x concentrated with 27.5 mM MgCl2 • MgCl2

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