SPINeasy RNA Kit for Tissue is a silica membrane spin column kit that enables quick and convenient purification of total RNA from a variety of tissue samples including cultured cells, fungi, animal tissues, and plant tissues. The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrep® instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds. With a simple workflow, the kit allows multiple samples to be processed simultaneously, without the use of toxic substances such as phenol and chloroform. Purified RNA is of high quality and integrity and suitable for RT-PCR and other downstream applications.
Application Notes:
The SPINeasy RNA Kit for Tissue quickly and efficiently isolates high-quality, total RNA from various animal tissues, plant tissues and tissue cultures in approximately 15minutes using a specialized Lysing Matrix A for cell lysis and SPIN columns for the purification process.
Usage Statement:
Research Use Only (RUO)
Key Applications:
Total RNA extracted from SPINeasy RNA Kit for Tissue can be used in several downstream applications, including RT-PCR, qPCR, Sequencing (NGS), and Northern Blot Analysis, Genomics, Molecular Biology
Key features and details
- Quick and convenient isolation of total RNA from a variety of tissue samples, including cultured cells, animal tissues, and plant tissues using spin column technology
- Lysing Matrix A and specially formulated Lysis Buffer for efficient and reproducible sample lysis with FastPrep instruments
- High-quality RNA suitable for downstream RT-PCR, qPCR, Sequencing (NGS), and Northern Blot Analysis
- Safe - no phenol/chloroform extractions
Alternate Names: SPINeasy RNA Extraction Kit for Tissues, Total RNA Extraction Kit for Tissues
Application Notes: The SPINeasy RNA Kit for Tissue quickly and efficiently isolates high-quality, total RNA from various animal tissues, plant tissues and tissue cultures in approximately 15minutes using a specialized Lysing Matrix A for cell lysis and SPIN columns for the purification process.
Base Catalog Number: 6535050
Brand: SPINeasy
Components: 50x Lysing Matrix A, 60 mL Lysis Buffer R, 12 mL Wash Buffer R, 10 mL Nuclease-free water, 1 vial DNase I, 5 mL DNase I Buffer, 50x Column R with collection tube
Hazard Statements: Hazard Class 8
Pack Size: 50 Preps
Personal Protective Equipment: Safety Goggles/Faceshield; Gloves
Sample Type: 10 - 30 mg Animal Tissue; 50-100 mg Plant Tissue; 1x107 Cultured Cells
Storage: Store at Room Temperature (15-25 °C) in a dry environment
Usage Statement Research Use Only (RUO)