SPINeasy Plasmid Midiprep Kit is a silica membrane spin column kit for the purification of high-copy and low-copy number plasmid DNA from 25 – 50 mL of transformed bacterial cultures. Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to release plasmid DNA. Cell debris, such as chromosomal DNA and proteins, are precipitated by neutralization and cleared through centrifugation. Subsequently, plasmid DNA in the supernatant is adsorbed on a silica membrane, washed, and eluted in a convenient process using spin column technology. Up to 1 mg of plasmid DNA can be purified through this simple workflow, which is immediately ready for routine molecular biology applications, including cloning, in-vitro transcription, PCR, and restriction digestion.
Application Notes:
The SPINeasy Plasmid Midiprep Kit is a silica membrane spin column kit for the purification of high-copy and low-copy number plasmid DNA from 25 – 50 mL of transformed bacterial cultures.
Usage Statement:
Research Use Only (RUO)
Key Applications:
In-vitro transcription, cloning, NGS, PCR, qPCR, restriction digestion, transformation, Genomics, Molecular Biology
Key features and details
- Spin column technology for fast and efficient purification of both high- and low-copy plasmids from 25 – 50 mL of bacterial cultures
- High-quality plasmid DNA is free of gDNA contamination following a modified alkaline lysis method
- Isolation of up to 1 mg of plasmid DNA suitable for a variety of downstream applications, including cloning, restriction digestion, and transformation
Alternate Names: SPINeasy Plasmid Midiprep Extraction Kit; Plasmid DNA Purification
Application Notes: The SPINeasy Plasmid Midiprep Kit is a silica membrane spin column kit for the purification of high-copy and low-copy number plasmid DNA from 25 – 50 mL of transformed bacterial cultures.
Base Catalog Number: 65390
Brand: SPINeasy
Components: 60 mL Resuspension Buffer SD1, 60 mL Alkaline Lysis Buffer SD2, 90 mL Neutralization Buffer SD3, 70 mL Wash Buffer DW1, 12 mL Wash Buffer DW2, 30 mL Elution Buffer SD, 600 µL RNase A, 25x Column SD with collection tubes
Hazard Statements: Hazard Class 8
Pack Size: 25 Preps
Personal Protective Equipment: Safety Goggles/Faceshield; Gloves
Sample Type: 25 - 50 mL bacterial (E. coli) cultures
Storage: Store at Room Temperature (15-30?), Dry environment
Usage Statement: Research Use Only (RUO).