The SPINeasy PCR Purification and Gel Extraction Kit is a silica -membrane spin -column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gels. Up to 23 µg of DNA of molecular weight ranging from 100 bp to 20 kb can be purified through a quick and simple process from most grades of agarose gels with expected recovery of 85%. Purified DNA is immediately ready for routine molecular biology applications.
Application Notes:
SPINeasy PCR Purification and Gel Extraction Kit is a silica-membrane spin-column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis.
Usage Statement:
Research Use Only (RUO)
Key Applications:
DNA clean-up; Primer, nucleotide, enzyme, agarose, salt removal from PCR, restriction digestion reactions, and from agarose gels for purified DNA, ready for downstream applications including cloning, NGS library preparation, in-vitro transcription, Genomics, Molecular Biology
Key features and details
- Spin column kit for quick and convenient DNA clean up from PCR and other enzymatic reactions
- Isolate and purify DNA fragments (100 bp – 20 kb) from agarose gels using spin column technology
- Purified DNA, free of primers, enzymes, nucleotides, agarose, etc., is ready for downstream molecular biology applications
Alternate Names: SPINeasy PCR Purification & Gel Extraction Kit; PCR and Enzymatic Clean-up Kit; DNA Clean-up Kit.
Application Notes: SPINeasy PCR Purification and Gel Extraction Kit is a silica-membrane spin-column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis.
Base Catalog Number: 65380
Brand: SPINeasy
Components: 50 mL Binding Buffer PG, 6 mL Wash Buffer PG, 10 mL Elution Buffer PG, 50x Column PG with collection tubes
Hazard Statements: Hazard Class 8
Pack Size: 50 Preps
Personal Protective Equipment: Safety Goggles/Faceshield; Gloves
Storage: Store at Room Temperature (15-30 °C), in a dry environment
Usage Statement: Research Use Only (RUO)