oasigPLUS Onestep Lyophilised 2x RT qPCR Master Mix is an optimised complete Master Mix for use in one step real time PCR. Use of a one step qPCR reaction, including a Reverse Transcription enzyme, reduces handling errors, and greatly shortens the time taken to obtain results. oasigPLUS OneStep Master Mix utilizes a tightly controlled temperature dependent Reverse Transcriptase enzyme. This improves multiplexing performance with higher consistency by minimizing uwanted non-specific priming and extension during room temperature set-up. The Master Mix contains a superior Reverse Transcriptase (as described above) and a hot start Taq DNA polymerase as well as buffer, dNTPs, MgCl2 and stabilisers at concentrations optimised for the enzymes and qPCR reaction. Once resuspended only the template RNA and primer/probe mix are required to complete the experimental set up for a perfect multiplex reaction.