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G-Biosciences

G-Sep™ Ni NTA Agarose Fast Flow

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Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.G-Sep™NI NTAAgarose Fast Flow is specifically designed for the purification of recombinant proteins fused to the 6x histidine (6XHis) tag.


The resin isspecifically designed for the purification of recombinant proteins fused to the 6X histidine (6XHis) tag expressed in bacteria, insects, and mammalian cells.The resin is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues.


G-Sep™ NI NTA Agarose Fast Flow (FF) resin is nickel ions immobilized onto highly cross-linked 6% agarose beads.The G-Sep™ NTA Agarose Fast Flow (FF) resins have high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. G-Sep™ Ni NTA Agarose Fast Flow (FF) resin using cobalt is also available

Features

  • Matrix: Cross-linked agarose beads, 6%
  • Bead form: Spherical, diameter 50-160μm
  • Spacer: Epichlorohydrin
  • Chelating Agent:nitrilotriacetic acid (NTA)
  • pH stability Working Range: 3-12
  • pH stability Cleaning-in-Place (CIP): 2-14
  • Maximum Flow Velocity: 450cm/h
  • Exclusion limit(globular proteins): 4 x 106
  • Physical Stability: Negligible volume variation due to changes in pH or ionic strength
  • Chemical Stability: Stable to all commonly used aqueous buffers: 6M urea, 8M guanidine hydrochloride,
  • Autoclavable: 121°C, pH 7, for 30 min
  • Storage Conditions: 2 to 8°C, 20% Ethanol

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Product Detail
Thomas No.
C753Y12
Mfr. No.
786-1041
Description
G-Sep™ Ni NTA Agarose Fast Flow, 25mL
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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