This is a special formulation of loading solution for protein gel electrophoresis. Special formulation Applications SDS-PAGE Western Blotting Analysis of DNA samples with high amounts of DNA binding proteins Kinetic experiments DNA agarose gel analysis after DNA restriction digestions,…

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …

L-glutamine is a cell culture supplement, and is required in cell culture for cellular growth. L-glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, gluthione, L-glutamate and other amino acids, as well as in protein synthesis and glucose production.…

Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications …

Protein Running Buffer, 10X is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE), and is the running buffer for TRIS-glycine gel electrophoresis. Contains 1% SDS Filtered through a 0.22 µm…

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators…

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Without Phenol Red Applications DPBS can be used to provide a…

LB Agar 1.5% Lennox provides all nutritional requirements of these organisms. LB Agar 1.5% Lennox contains half the sodium chloride level of the Miller formulation of LB Agar. Just autoclave to melt and pour. Applications LB Agar 1.5% Lennox is nutritionally rich. It was developed by…

Sodium Chloride, 0.85% is a saline solution at 0.85% prepared with water used for cell culture. Made with cell culture grade water The sodium chloride is a source of chlorine and sodium necessary to the production of components like sodium carbonate, sodium hydroxide or chlorate ions. …

Molecular Biology Water is prepared by reverse osmosis, passed through fine carbon, deionized through two resin beds, and serially filtered through 0.1 µm positively charged membranes, to yield a product with an endotoxin level of less than 0.03 EU/mL. DNase, RNase and protease tested Endotoxin…

EMEM (Eagle’s Minimal Essential Medium) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without L-Glutamine Applications EMEM has a higher amino acid concentration and 2 to 5 times greater…

Deionized, Ultra Pure, Endotoxin Free, Sterile QBI's Cell Culture Grade Water is high quality water for use as a solvent in the preparation of cell culture media and laboratory reagents. Available in many sizes to meet your needs, QBI's Cell Culture Grade Water for Cell Culture…

EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and…

Nutritionally rich media designed for growth of pure cultures of recombinant strains. 10 g/L of salt formulation Autoclave sterilized LB Agar, Miller and LB Broth, Miller are based on LB Medium as described by Miller for the growth and maintenance of E. coli strains used in molecular…

LB Agar Plates are used for the growth and maintenance of E. coli strains used in molecular microbiology procedures. Applications The following variations of LB Agar Plates are nutritionally rich media designed for growth of pure cultures of recombinant strains. LB Agar plates are suitable…

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. Ready to use solution Applications Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis…

QBI's Serum Free Cryo Medium is a sterile, 2X concentrated freezing medium containing DMSO  developed for the cryopreservation of cells grown under serum free conditions. Serum Free Cryo Medium is an animal-free, protein-free, chemically defined medium containing DMSO and other…

QBSF-60 is a proprietary serum-free medium that supports the expansion of human hematopoietic stem/progenitor cells. QBSF-60 supports CD34+ cells derived from normal bone marrow, cord blood, peripheral blood stem cells mobilized with cytokines for ex-vivo expansion and differentiation to…

Super Broth is used for the mass cultivation of Escherichia coli for high yield protein and plasmid DNA production. Has 220% more peptone and 300% more yeast extract than LB Autoclave sterilized Applications Super Broth is the best commonly used E. Coli growth medium for plasmid…

Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications …

Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride and provide convenient and highly purified solutions. Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its…

TNE Buffer is often used as a base material to generate lysis buffers. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications More specifically, TNE Buffer is useful for: isolation of proteins from yeast cells as a lysis buffer, resuspension of pelleted virus…

DMEM is one of the most widely used modifications of Basal Medium Eagle (BME). DMEM was originally developed as a richer version of BME; with 4 times higher concentrations of amino acids, vitamins and some additional components than BME. DMEM is commonly used for the culture of normal and…

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. Without EDTA Applications Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223…

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With Stabilized L-glutamine Applications DMEM is one of the most widely used modifications of Basal Medium Eagle (BME). DMEM was originally developed as a…

DNA Gel Loading Solution, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer. …

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction…

Applications The FBS in the Cryopreservation Medium improves the post-freezing recovery and survival after cryopreservation, while DMSO preserves the cells during freeze. Cryopreservation Medium with Fetal Bovine Serum (FBS) and Calf Serum is a complete cryopreservation medium with 10% DMSO.

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H. Used with SOC medium Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure,…

Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Filtered through a 0.22 µm membrane DNase,…