Lonza
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FlashGel TM Loading Dye (5X) is sample buffer optimized for preparation of DNA and native RNA samples used with the FlashGel TM System. FlashGel TM Loading Dye (5X) is provided in a 5X concentration. Simply prepare and load samples, watch bands migrate and get data in as little as 2…
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Reliant™ Gels
LonzaThe Reliant™ Gel System consists of precast agarose minigels for rapid and reproducible resolution of DNA fragments from 8 bp to 10 kb. Each precast agarose gel is precision cast using our SeaKem® Gold, NuSieve™ Hi-Res or NuSieve™ 3:1 Agarose and are available…
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AccuGENE™ Water
LonzaAccuGENE™ Water is considered a “fit for purpose” Type I water and is created for use specifically in molecular biology applications.
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PCR Products on a NuSieve™ 3:1 Agarose Gel – A 550 bp sequence from lambda DNA was amplified (25 cycles) using primers and Taq DNA polymerase supplied in the GeneAmp® kit (Roche Molecular Systems). PCR products and controls were electro phoresed on a 4% NuSieve™ 3:1 agarose…
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AccuGENE™Tris CL or Trishydroxymethyl aminomethane is a buffer with a range (7-9) that coincides with the physiological pH typical of most living organisms. It is the “T” in TAE, TBE and TE Buffers, and one of the most common starting chemicals when creating a biological buffer.…
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SimplyLoadTM DNA Ladder
LonzaLonza SimplyLoad™ DNA Ladders are ready-to-load directly on your gel to maximize your time.. SimplyLoad™ DNA Ladders are supplied at ready-to-load concentrations in TE Buffer and 1X Ficoll® sample loading buffer containing Bromophenol blue and Xylene cyanol FF so you can get…
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SeaPlaque™ Agarose
LonzaSeaPlaque™ Agarose is the original low melting temperature agarose. This molecular biology grade agarose produces gels with greater sieving properties and higher clarity than standard melting temperature agarose. The low melting temperature of SeaPlaque™ Agarose makes it ideal for…
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AccuGENE™ TBE (Tris hydroxymethyl aminomethane) is made from Tris, EDTA and Boric acid. The addition of borate makes TBA a better conductive medium than TAE so it less prone to overheating and better for longer runs. Borate does inhibit some enzymatic reactions, so it is not often used if the…
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Reliant™ Gels
LonzaThe Reliant™ Gel System consists of precast agarose minigels for rapid and reproducible resolution of DNA fragments from 8 bp to 10 kb. Each precast agarose gel is precision cast using our SeaKem® Gold, NuSieve™ Hi-Res or NuSieve™ 3:1 Agarose and are available…
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MetaPhor™ Agarose
LonzaDNA ladders with 4 bp or 8 bp step sizes were prepared by ligation of Bgl II linkers. Aliquots of 0.8 µgof the ladders were separated on a 3.5% MetaPhor™ Agarose gel in a horizontal format and compared to an 8% polyacrylamide gel run in a vertical format in TBE buffer.
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FlashGel™ RNA Cassettes
LonzaThe FlashGel TM RNA Cassettes are optimized for the unique requirements of RNA, and are a fast and sensitive tool for analysis of sample integrity. The system is recommended for verification and analysis of total RNA, quick checks of native RNA and checking RNA degradation and mRNA purity. RNA…
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SYBR® Green stains are fluorescent dyes used to detect DNA and RNA. They are more sensitive and less mutagenic than standard stains, yet they provide excellent signal-to-noise ratio with minimal background. SYBR® Green I detects as little as 60 pg dsDNA and 1 ng oligonucleotides in agarose…
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AccuGENE™ Tris Glycine Native Buffer is a Tris buffer with added glycine. It is commonly used in electrophoresis of proteins in their “Native” , non-denatured state thus preserving the molecules' higher-order structure. When SDS is added, it denatures the proteins allowing…
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Positive control for MycoAlert ® PLUS Mycoplasma Detection Kit and MycoAlert ® Mycoplasma Detection Kit
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FlashGel Recovery Kit
LonzaThe FlashGel TM Recovery Kit gets you straight to your recovered samples. Simply load samples, watch bands migrate and get recovered samples in as little as 5 minutes. The kit contains the cassettes and accessories needed to begin recovering samples from FlashGel TM Recovery Cassettes. Say…
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SeaKem® Gold Agarose
LonzaSeaKem® Gold Agarose is a very high gel strength, low EEO, standard gelling temperature agarose. This Genetic Technology GradeTM Agarose is for rapid resolution of megabase DNA by pulsed field gel electrophoresis (PFGE). Due to its low EEO, the electrophoretic mobility of DNA in SeaKem®…
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NuSieve™ 3:1 Agarose
LonzaNuSieve TM 3:1 Agarose is a molecular biology grade, standard melting temperature agarose that yields strong gels for fine resolution of small DNA, RNA, and PCR products ≤ 1 kb. NuSieve TM 3:1 Agarose is designed for analytical electrophoresis rather than in gel reactions or downstream…
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The ProSieve™ ProTrack™ Dual Color Protein Loading Buffer protects proteins from heat degradation during the sample preparation step, as well against pH changes during the SDS-PAGE run. Some proteins are sensitive to pH changes that result from temperature fluctuations during…
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RNA Marker
LonzaRNA markers consist of RNA transcripts from 0.5 to 9 kb, in sizes of 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, and 9 kb. These RNA standards may be denatured with standard procedures, and can be visualized on Northern blots with labeled lambda sequence. Markers are provided at a concentration of 1 mg/ml.…
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DNA LOADING BUFFER
LonzaFicoll® based loading buffer containing Ficoll®, EDTA, xylene cyanol, and bromophenol blue. Ideal for improving resolution and separation of DNA fragments.
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Formaldehyde denaturation is suitable when samples are to be recovered from agarose gels. Our Formaldehyde Sample Buffer contains formaldehyde, formamide, and tracking dyes in MOPS Buffer. Supplied in 1.0 ml vials
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5X TBE BUFFER
LonzaAccuGENE™ TBE (Tris hydroxymethyl aminomethane) is made from Tris, EDTA and Boric acid. The addition of borate makes TBA a better conductive medium than TAE so it less prone to overheating and better for longer runs. Borate does inhibit some enzymatic reactions, so it is not often used if the…
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Checking Sample Quality with the FlashGel TM System for RNA – Sample degradation is visible at low levels on the FlashGel TM System for RNA. FlashGel TM RNA Cassette run for 8 minutes at 225 V, followed by 20 minute hold prior to imaging. Lane 1: RNA marker (Lonza); Lane 2: 250 ng E. coli…
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The ProSieve™ QuadColor™ Protein Marker is a mixture of 12 recombinant, highly purified proteins with molecular weights of 4, 6, 10, 15, 25, 40, 55, 70, 100, 140, 170, 250, and 300 kDa. the proteins are individually prestained using four different dyes, producing a brightly colored…
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AccuGENE™ 10X PBS
LonzaAccuGENE TM 10x PBS (Phosphate buffered saline) is a buffer solution commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate and sodium chloride. The osmolarity and ion concentrations of PBS are isotonic, so it is often used for protein and…
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SeaKem® HGT Agarose
LonzaSeaKem® HGT Agarose is a high gelling temperature agarose that exhibits high gel strength and clarity and is primarily used the electrophoreisis of proteins. Specifically designed for applications such as counter-immunoelectrophoresis and crossed-immunoelectrophoresis, SeaKem® HGT Agarose…
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GelBond® PAG Film
LonzaGelBond® PAG Film is a transparent, flexible polyester film designed to support polyacrylamide or MDE™ Gels. The acrylamide monomers covalently attach to the coating on the film during the polymerization reaction. Gels remain permanently attached to the film through electrophoresis…
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FlashGel System
LonzaThe FlashGel™ System (Cat. No. 57067) contains a FlashGel™ Dock, a FlashGel™ Camera, a pack of 1.2% FlashGel™ DNA Cassettes, Loading dye and Marker.
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To ensure you obtain accurate and reliable results from your cell culture experiments, it is essential that you regularly test culture samples for mycoplasma contamination. Mycoplasma detection is challenging because contamination does not cause pH or turbidity changes of your cell culture medium,…