LGC, Biosearch Technologies
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EZ-TN5 TRANSPOSASE 10 U
LGC, Biosearch TechnologiesEZ-Tn5™ Transposase is a hyperactive Tn5 transposase for random insertion of an EZ-Tn5 Transposon into any cloned DNA in vitro or for preparing an EZ-Tn5 Transposome for random insertion of a EZ-Tn5 Transposon into the genomic DNA of living bacteria.
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Ampligase 10X Reaction Buffer 5 ml
LGC, Biosearch TechnologiesAmpligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures
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TG1 Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. The highest efficiency TG1 competent cells available: =4 × 10^10 cfu/µg. TG1 Electrocompetent Cells have an amber suppressor strain (supE) prepared as highly efficient electrocompetent cells for phage display library…
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Exonuclease VII 250 U @ 10 U/µl
LGC, Biosearch TechnologiesExonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5´′→3´ and 3´′→5´ exonuclease activities.
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E. cloni 10G 96-well Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of…
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NxSeq UltraLow DNA Library Kits
LGC, Biosearch TechnologiesThe NxSeq UltraLow DNA Library Kit is used to build DNA fragment libraries for Illumina sequencers. It requires only 50 pg to 75 ng of input DNA. This 96 rxn kit is optimized for library prep in 96-well plates and requires a NxSeq HT Dual Indexing Kit to complete library construction.
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LavaLAMP DNA Master Mix
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP (loop-mediated isothermal amplification) reactions. LAMP kits are commonly available as multi-component kits that require optimization (e.g. MgSO4, betaine, enzyme as well as temperature, primer…
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OverExpress Chem Comp Cells
LGC, Biosearch TechnologiesLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins…
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Ampliscribe T7 High Yield Transcription Kit 50 Rxns
LGC, Biosearch TechnologiesAmpliScribe™ T7 High Yield Transcription Kits are specially formulated to produce >20-fold more full-length RNA transcript than conventional in vitro transcription reactions.
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TRANSFORMAX EC100 CHEMICALLY COMP E. COLI 10 rxns
LGC, Biosearch TechnologiesChemically Competent E. coli cells are ideal for most cloning applications. The cells provide very high transformation efficiency.
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Ribonuclease R (RNase R) 250 U
LGC, Biosearch TechnologiesRibonuclease R (RNase R) from E. coli is a magnesium-dependent 3´′→5´ exoribonuclease that digests essentially all linear RNAs but does not digest lariat or circular RNA structures.
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OmniCleave Endonuclease 50,000 U
LGC, Biosearch TechnologiesOmniCleave™ Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides.
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Green Fluorescent Dye 500 rxns
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Component Kit with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.
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MasterAmp Tth DNA Polymerase 250 U
LGC, Biosearch TechnologiesMasterAmp™ Tth DNA Polymerase is a recombinant thermostable DNA polymerase for use in PCR. This enzyme has optimal DNA synthetic activity at temperatures above 70°C and can be used at temperatures up to 95°C.
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MasterAmp 10X PCR Enhancer 10 ml
LGC, Biosearch TechnologiesFor use with the MasterAmp™ PCR Optimization Kits.
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T4 Polynucleotide Kinase, Cloned 3,000 U
LGC, Biosearch TechnologiesT4 Polynucleotide Kinase (PNK) catalyzes the transfer of the ?-phosphate from ATP to the 5´ hydroxyl of ssDNA and dsDNA, RNA, and nucleoside 3´ monophosphates.
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RNase I 1,000 U
LGC, Biosearch TechnologiesRNase I degrades single-stranded RNA to nucleoside 3´ monophosphates via 2´,3´ cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3 unlike RNase A, which cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70°C…
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Rec J Exonuclease 250 U
LGC, Biosearch TechnologiesRec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´′→3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.
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RNase-Free DNase I
LGC, Biosearch TechnologiesRNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation.
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2'-dNTP (All 4 Mixed) 10 µmole
LGC, Biosearch TechnologiesSterile, neutral pH solutions of dATP, dCTP, dGTP, and dTTP are available either as individual nucleotides or a mixture of all four nucleotides for use in DNA synthesis.
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NxSeq Single-cell RNA-seq Beads
LGC, Biosearch TechnologiesConsistent, high quality beads for single-cell mRNA sequencing applications using Drop-seq1 methods Consistent: Each lot of beads has tightly controlled bead size and produces consistent performance lot to lot Powerful: The 14 base UMI enables sequencing and identification of more unique…
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Sbeadex viral RNA Purification Kit
LGC, Biosearch Technologiessbeadex viral RNA purification kits are magnetic-bead based kits are validated for research use only using AccuPlex™ reference material (SeraCare), replicating a real-life clinical sample as far as practically possible, and are compatible with Biosearch Technologies’ COVID-19 detection kit.…
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Baseline-ZERO DNase 5,000 U
LGC, Biosearch TechnologiesBaseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.
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NxGen T4 DNA Ligase
LGC, Biosearch TechnologiesT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
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NxGen M-MuLV Reverse Transcriptase
LGC, Biosearch TechnologiesM-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3'′→5' proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.
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E. cloni 10GF’ Electrocompetent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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Expression Recovery Medium (Lactose Minus)
LGC, Biosearch TechnologiesThe LGC expression recovery medium with a capacity of 12 mL helps in the stabilization and transformation of protein expression clones in chemical laboratories and research facilities.
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Ampliscribe T7-Flash Transcription Kits
LGC, Biosearch TechnologiesThe AmpliScribe™ T7-Flash™ Transcription Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.
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Endura ElectroCompetent Cells
LGC, Biosearch TechnologiesLucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be…
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TRANSFORMAX EPI300-T1R ELECTRO E. COLI 20 rxns
LGC, Biosearch TechnologiesPhage T1-resistant electrocompetent cells specially engineered to regulate the copy number of CopyControl Fosmid clones.
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Copycontrol Induction Solution 25 Ml
LGC, Biosearch TechnologiesPrepare unbiased fosmid libraries of approx. 40 kb that can be maintained at single-copy number and induced to high-copy number as needed when grown in the TransforMax EC300 E. coli.
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RapiDxFire Hot Start Taq DNA Polymerase
LGC, Biosearch TechnologiesRapiDxFire™ Hot Start Taq DNA Polymerase is recombinant Taq DNA Polymerase from Thermus aquaticus bound to select blockers, which prevents unwanted, nonspecific amplification during reaction set up at temperatures less than 60 °C. Enzyme activity is restored after a short 15 second burst…