NUCLEIC dotMETRIC™ kit provides a quick and accurate method for detecting and measuring DNA, RNA and oligonucleotides. The system uses a unique combination of proprietary reagents and test strips and is an alternative to spectrophotometry as no expensive equipment or cuvettes are necessary.…

ProteCEASE™ is a dry format version of our ProteaseARREST™ for large scale preparative applications and for those who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant,…

MW: 544.43 C₂₂H₂₉N₇O₅ · 2HCl Mode of action and Anti-microbial spectrum: Puromycin is a protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3′-terminal end of aminoacyl-tRNA. It prevents…

Mayer’s Hemotoxylin Stain, modified. Modified for enhanced shelf-life whileavoiding use of Chloral Hydrate. Manufactured with BSC certified reagents.

Features Synonym: Poly(ethylene glycol) CAS#: 25322-68-3 Molecular Formula: (C₂H₄O)nH₂O

This kit is designed to teach 8 groups of four students the principles of Protein Electrophoresis. The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution Prepare samples for PAGE electrophoresis Separate and distinguish proteins by…

Features CAS#: 7647-14-5 Molecular Formula: NaCl Molecular Weight: 58.44

Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligand s containing amine groups, thiol or hydroxyl groups. Epoxide-Acivated Agarose is prepared by immobilization of…

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups…

Electroelution of nucleic acids and proteins has many advantages as it avoids centrifugation, vortexing, heating, precipitation and allows minimal manipulation of samples. Electroelution normally involves dialysis tubing, which results in extreme dilution of precious samples. G-Capsule™ is a…

G-Trap™ SP Agarose Fast Flow is ready to use prepacked column used for seperation of proteins and other biomolecules based on charge. The resin in G-Trap™ SP Agarose Fast Flow is a strongcation exchanger composed of highly crosslinked 6% agarose beads coupled with sulphopropyl (SP)…

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to…

L-Citrulline 7-amido-4-methylcoumarin hydrobromide; H-Cit-AMC·HBr   MOLECULAR BIOLOGY GRADE White to off-white crystalline powder. A sensitive fluorogenic substrate for citrulline ureidase, yielding a blue solution upon cleavage. Store at -15°C and protect from…

Quality DNase and RNase free reagent solution for yeast research.   Features Synonym: Sorbitol F solution CAS #: 50-70-4 Molecular Formula: C₆H₁₀O₆ Molecular Weight: 178.14    

For use in molecular biology applications, PCR reactions, 2D electrophoresis and mass spectrometry. Molecular Grade Water™ is suitable for use in molecular biology applications which demand a high quality of water and assurance that the water is free from DNase, RNase and protease…

HOOK™ 6X His Protein Spin Purification kit allows for the rapid purification of soluble, 6X His tagged protein from yeast cultures.The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and…

Carbonyl groups, including aldehydes, ketones and glyoxals, react with amines to form Schiff base intermediates that are in equilibrium with their free forms.The labile Schiff’s base interaction can be stabilized by chemical reduction. If the Schiff’s base is formed between an aldehyde…

A proprietary mixture of Formaldehyde, EDTA and Blue Dextran that is designed for use in loading DNA samples onto denaturing polyacrylamide gels. Applications Loading DNA samples onto denaturing polyacrylamide gels

Our Bacterial Gram Staining kit is supplied with three strains of bacteria and all the necessary prealiquoted components to carry out the Gram staining to determine Gram positive and negative bacteria. The Gram staining method was first described in 1844 by the Danish bacteriologist Hans…

HOOK™ Sulfo-NHS-SS-Biotin reacts with protein primary amines.Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…

A highly efficient grinding resin that is pre-aliquoted into 1.5ml grinding tubes and is supplied with matching pestles. The resin is designed for optimal grinding of biological samples for the extraction of both proteins and DNA. The resin is a neutral abrasive material that does not bind…

An ELISA kit for the detection of ENG (Human). This uses Sandwich ELISA, Double Antibody and has a sensitivity of 0.094ng/ml.

Dialysis is the process of separating molecules in solution by the difference in their rates of diffusion through a semi permeable membrane, such as dialysis tubing or Tube-O-DIALYZER ™ dialysis caps.Molecules small enough to pass through the dialysis membrane move across the membrane in…

G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It exhibits high selectivity, superb resolution, low non-specific adsorption and robust chemical stability. Quickly desalts, removes contaminants and…

This lab activity teaches the use of highly specific matching properties of antigen-antibody for detection of antigenic or antibody proteins in complex samples.   One such immunodetection method is widely known as Dot Blot. The method involves applying small volumes of antigens or test…

A unique stain for reversibly staining protein on nitrocellulose and PVDF transfer membranes. BLOT-FastStain™ only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility. The staining procedure takes 10 minutes and has a…

Synonyms: TE Buffer, Tris-EDTA Buffer, T10E1 Buffer The TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA. The TE Buffer [10X] is offered in 0.5 and 1L…

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major problem for researchers…

The Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct The study of the lac operon has played an important role in…

High quality proteomic grade β-mercaptoethanol. β-mercaptoethanol, a popular reducing agent, is offered in 5ml and 100ml bottles. Features Synonym: β-Mercaptoethanol CAS#: 60-24-2 Molecular Formula: C₂H₆OS Molecular Weight: 78.13 …

Firefly Luciferase Assays are one of the widely used reporter assays to detect and measure gene expression, function and regulation. G-Biosciences Lumino™ Firefly Luciferase Assay is designed for rapid and high sensitive detection or quantification of firefly luciferase reporter enzyme…

The Bicinchoninic Acid (BCA) Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the…