Thomas No. C995V29-V30 Tris-Borate-EDTA (TBE) Buffer, 10X Liquid Concentrate A buffer widely used for nucleic acid electrophoresis Technical Information: 1X buffer contains 0.089M Tris, 0.089M Borate and 0.002M EDTA pH (1X) @ 25ºC : 8.2-8.4 1L of concentrate makes 10L of 1X…

The bioWORLD bioPlus™ 0.1 M sodium phosphate is composed of sodium phosphate monobasic, sodium phosphate dibasic heptahydrate and water. It is mainly used as a saline laxative. It is also used in manufacturing detergents, soaps and fertilizers.

Reagent grade A strongly polar organic solvent Refractive index: 1.423- 1.431 Hazardous Materials Description: N,N-Dimethylformamide Hazard Class or Division: 3 Identification Numbers: UN2265 Packaging Group: III *Applies additional hazardous materials handling…

Guilbert and Iscove published their modifications to Dulbecco's Modified Eagle's Medium (DMEM) in 1976. Their modification included the supplements of selenium, additional vitamins and amino acids, sodium pyruvate and HEPES buffer. In addition, ferric nitrate was replaced with potassium…

It contains one oxygen and two hydrogen atoms connected by covalent bonds. It is commonly used in chemical reactions as a solvent or reactant and less commonly as a solute or catalyst. Application: For Research & Laboratory Use Only

Washing buffer for peroxidase conjugates in Western Blotting. 1X PBS with Ca++ and Mg++ pH 7.4 0.1 (25°C)

Acrylamide and bis-acrylamide are major ingredients in polyacrylamide gels, a commonly used matrix for electrophoretic separation of nucleic acids and proteins. Fragments of DNA produced by various molecular biology techniques (eg. restriction endonuclease digestion, dideoxy sequencing, or the…

Potassium phosphate buffer has a concentration of 0.1M and a pH of 7.2. It contains 28.3% of KH2PO4 (monobasic) and 71.7% of K2HPO4 (dibasic). Phosphates have a high buffering capacity and are soluble in water. They inhibit many enzymatic reactions and procedures of molecular cloning. Phosphates…

Ultra Pure Grade pH at 25°CC: 7.4+/- 0.1 DNase Activity: None Sterility: Passes Storage: RT Hazard: Irritant

May be used to test carbon assimilation in the presence of a carbon source like glucose, galactose, or raffinose Based upon the formulation of Yeast Agar except that Ammonium Sulfate is the sole nitrogen source and that addition of carbon source is required pH: 5.4 Dehydrated…

Blot blocking buffer used to block non-specific binding site for antibodies on the membrane. 1% Gelatin in PBS

PBS (Phosphate Buffered Saline) has a concentration of 1X and can be sterilized by autoclaving. It contains 726.0 mg/L NaH2PO4, 9, 000.0 mg/L NaCl, 210.0 mg/L KH2PO4 and has a pH of 7.2. PBS is a water-based salt solution that helps maintain a constant pH. It is isotonic and non-toxic to most cells…

It is a toxic organic compound used as an automotive antifreeze and a precursor to polymers. It is used as plasticizers in adhesives, lacquers and enamels. It also used as coolant and heat transfer agent. High Purity Grade Acidity: <=0.001% Iron: <=0.0005% Moisture (KF):…

A commonly used analytical biochemistry dye and P2X7 antagonist Stain for location and detection of proteins. Protocol: After SDS-PAGE, transfer the gels in fixing solution (30% methanol and 7% acetic acid) for 30 min. under constant agitation. Replace the fixing solution, submerge the…

IBS/Casein (Thomas Nos. C994C53 and C994C54) All blocks and diluents are supplied in a 10X concentration of physiological saline buffer. Sold as 2 x 100mL or 500mL. Technical Information: pH range: 7.0 (+/- 0.1) based upon an Imidazole physiologic buffer …

Bradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…

Thomas No. C995V08-V09 bioWORLD running buffers are prepared using high quality reagents so you can be asured of accurate test results Technical Information: Dilute with deionized water to make 1X 1X Concentration Contains: 7 mM Phosphoric Acid pH: 2.4 Thomas No. C995V10-V11…

All bioWORLD running buffers are prepared with high quality reagents.

Propylene Glycol is a common solvent used in biochemistry for its low freezing point and non-toxicity. When used in PCR, Propylene Glycol preserves nucleic acids for better results. It has been observed to greatly increase the stability of DNA for long-term preservation. Molecular Biology Grade…

Tris Acetate Buffer has a 1M concentration, pH of 8.5, and is sterile. Tris Acetate Buffer is a common biological buffer used in biological reactions.

Thomas No. C995V18-V19 Tris-Acetate-EDTA Technical Information: A common buffer for electrophoresis Each Ready-Pack prepares 1 L of 10X concentrate 1 pack per case pH 8.3 +/- 0.1 Thomas No. C995V20-V21 Tris-Acetate-EDTA (TAE) Buffer Technical Information: …

Glycerol (or glycerine, glycerin) is a polyol compound. It is colorless, odorless,viscous liquid that is widely used in biological applications. Synonym: glycerin Common reagent used in tissue culture media for bacteria and enzyme storage. Useful for mammalian cell transfection and…

Also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue,…

Common buffer for biological sciences. 0.1M MES (2-[morpholino]ethanesulfonic acid) and 0.9% NaCl Appearance: Liquid Melting Point: >300°C (lit.)(dec.) PH Value: 6.1 RTECS Number: QE3479500

A non-ionic nondenaturing detergent used in buffers to solubilize membrane proteins. Commonly used in gels for PAGE isoelectric focusing. Reagent Grade Heavy metals (such as Pb): Also known as Sorbitan Monooleate

Acid fast bacillus stain uses carbol fucshin dye (Ziehl Neelsen method) for identification of acid fast organism.

PBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Phosphate Buffered Saline with Tween 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter…

Common reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols. DNAse and RNAse free, ready to use 1X solution Technical Information: NaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)