Boston BioProducts, 7.6 ± 0.15
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Phosphate Buffered Saline (10X, pH 7.6)
Boston BioProductsComponents: Sodium Phosphate Dibasic(100 mM), Potassium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.
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Tris-Buffered Saline (10X, pH 7.6)
Boston BioProductsComponents: Tris Base / Tris-HCl(250 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.
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Sodium Phosphate Buffer (1 M, pH 7.6)
Boston BioProductsComponents: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(1M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.6 ± 0.15 Storage: Room Temp.
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HEPES Buffer (1 M, pH 7.6)
Boston BioProductsComponents: HEPES(1 M), Water(Rest), Sodium Hydroxide(for pH adjustment) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.6 ± 0.15 Storage: 2-8°C
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HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH)
Boston BioProductsComponents: HEPES(1 M), Potassium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water, pH adjusted with KOH and filtered through 0.22-micron filter. pH: 7.6 ± 0.15 Storage: 2-8°C
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Tris Buffered Saline-Tween (10X, with 0.5% Tween-20, pH 7.6)
Boston BioProductsComponents: Tris Base / Tris-HCl(500 mM), Sodium Chloride(1.5 M), Tween-20(0.5%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.
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Tris Buffered Saline-Wash Buffer (10X, pH 7.6)
Boston BioProductsComponents: Tris Base / Tris-HCl(500 mM), Sodium Chloride(1.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.
