Western Blotting & ELISA

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  • HeLa Whole Cell Lysate, 500µg, Liquid

    Rockland Immunochemicals Inc

    Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes…

  • This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™…

  • Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes…

  • Anti-RAT IgG [H&L] (GOAT) Antibody

    Rockland Immunochemicals Inc

    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

  • Black plastic hinged box ideal for the incubation, blocking, and washing steps for Western Blotting using Fluorescent Conjugated Antibodies such as DyLight Conjugated Secondary Antibodies or IRDye Antibody Conjugates. This large box is best for volumes between 10mL and 30mL.

  • Anti-GOAT IgG (H&L) (DONKEY) Antibody

    Rockland Immunochemicals Inc

    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

  • Protein A Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.

  • The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable…

  • F(ab')2 Anti-BIOTIN (GOAT) Antibody

    Rockland Immunochemicals Inc

    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

  • Ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95° C for 5 minutes and rapidly cool. If…

  • Magnetic separator unit can be used to hold, and separate magnetic particles.

  • Anti-Human IgG (H&L) DyLight 549 is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission…

  • Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 655 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging,…

  • F(ab')2 Anti-CAT IgG [H&L] (RABBIT) Antibody

    Rockland Immunochemicals Inc

    Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

  • Suitable for use in peroxidase enzymatic labeling experiments. After reconstitution, dilute sample with PBS containing 5% normal serum from same host bridging antibody.

  • Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging,…

  • BOVINE SERUM ALBUMIN 30% Solution

    Rockland Immunochemicals Inc

    BOVINE SERUM ALBUMIN 30% Solution is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody, and use as a blocking agent to reduce non-specific binding.

  • Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging,…

  • Streptavidin Peroxidase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).

  • BOVINE SERUM ALBUMIN

    Rockland Immunochemicals Inc

    Bovine Serum Albumin - Fraction V is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding for FACS, IF, IHC,…

  • Anti-GUINEA PIG IgG (H&L) (GOAT) Antibody

    Rockland Immunochemicals Inc

    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

  • Protein G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

  • PROTEIN A DyLight™

    Rockland Immunochemicals Inc

    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™…

  • TrueBlot® Protein A Magnetic Beads

    Rockland Immunochemicals Inc

    TrueBlot® Protein A magnetic beads can be used for immunoprecipitation (IP) and Co-IP experiments as well as for antibody purification. For IP, target specific antibody is incubated with cell lysate. Protein A magnetic beads are then incubated with antigen-antibody complex at room temperature,…

  • This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

  • STREPTAVIDIN

    Rockland Immunochemicals Inc

    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

  • BOVINE SERUM ALBUMIN

    Rockland Immunochemicals Inc

    Bovine Serum Albumin - Fraction V is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding for FACS, IF, IHC,…

  • This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

  • This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using ONPG as a substrate for 30 minutes at room temperature.  A working dilution of 1:100 to 1:3,000 is suggested for this product. 

  • This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™…

  • Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes…

  • This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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