Western Blotting & ELISA

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G-Biosciences Western Blotting & ELISA

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  • BLOK™ BSA

    G-Biosciences

    Protein solutions are used for blocking excess binding sites in ELISA, blotting, immunohistochemistry and other immunochemical applications. Each blocking solution will impart certain characteristics on the assay system. Our BLOK™ BSA is a 10% solution of BSA (Bovine Serum Albumin) …

  • Protein-Free Blocking Buffer does not contain protein; it is a proprietary formation of non-protein agents that eliminates non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free Blocking Buffer eliminates problems…

  • Nitrocellulose Membranes

    G-Biosciences

    For user's convenience, we offer pre-cut transfer membranes and padding for Western blot transfer procedures. Pre-cut membranes are supplied sandwiched between blotting paper padding. Simply soak the membrane in transfer buffer and assemble with the gel in a transfer cassette. …

  • Enhancer™ allows for the rapid application of up to 384 protein or nucleic acid samples on to a 12 x 9cm membrane for subsequent analysis. Samples are applied to a nitrocellulose or nylon membrane by capillary action, resulting in concentration of sample due to minimal diffusion. …

  • Swift Membrane Stain™

    G-Biosciences

    Swift™ Membrane Stain is a unique, proprietary (patents pending), rapid, reversible, ready-to-use membrane stain for proteins on nitrocellulose or PVDF membranes. Swift™ Membrane Stain stains proteins faster and with 500 times more sensitivity than the routinely used Ponceau-S stain and…

  • Well-Coated ™ Protein A plates are designed to bind the constant (Fc) region of immunoglobulins ensuring that the antigen binding domain of the antibody is orientated away from the plate, offering maximum exposure of the binding site. The immunoglobulin orientation on the Well-Coated ™…

  • Well-Coated ™ Sulfhydryl Binding plates are designed to specifically bind free sulfhydryls of peptides, proteins and other molecules. The Well-Coated ™ Sulfhydryl Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other…

  • Well-Coated ™ Protein A/G plates are designed to bind the constant (Fc) region of immunoglobulins ensuring that the antigen binding domain of the antibody is orientated away from the plate, offering maximum exposure of the binding site. Protein A-G contains 4 binding sites from protein A and…

  • PVDF Membranes

    G-Biosciences

    Our PVDF (polyvinylidene) membranes are pre-cut membranes for Western transfers, or Western blotting.The PVDF membranes bind biomolecules, including proteins, through hydrophobic interactions The presence of the 0.45µm pore sizes increases the surface binding area. Typical…

  • The Swift™ Western Blotting System is a unique system that reduces the blocking and antibody incubations on Western blot membranes from >4 hours to <60 minutes. Using a combination of proprietary wash and diluent buffers and our highly sensitive femtoLUCENT™ …

  • SWIFT™ Transfer Pads

    G-Biosciences

    Western blot analysis of proteins is a routine and commonly used technique in research laboratories, with 3 major drawbacks. The first is the amount of time taken to transfer the proteins to a protein binding membrane; the second is the variable efficiency of the transfer and the third is problems…

  • femtoCHROMO™-AP Kit

    G-Biosciences

    femto CHROMO™ AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X)…

  • Well-Coated™ Glutathione plates are designed to specifically bind GST (Glutathione S-Transferase) tagged proteins and peptides. The plates have immobilized glutathione and can be used for the isolation of GST-tagged proteins direct from bacterial lysates and then used for subsequent ELISA…

  • Western ReProbe PLUS

    G-Biosciences

    Based on our popular Western ReProbe™ , the modified formulation allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies…

  • Well-Coated ™ Protein G plates are designed to bind the constant (Fc) region of immunoglobulins ensuring that the antigen binding domain of the antibody is orientated away from the plate, offering maximum exposure of the binding site.The immunoglobulin orientation on the Well-Coated ™…

  • BLOK™ Casein

    G-Biosciences

    Convenient ready-to-use blocking solutions for Western blots, dot Blots & ELISA Features 1% Casein (w/v) solution for blocking Westerns, ELISA and dot Blots Ready-to-use Available in your choice of TBS or PBS buffers.

  • picoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. picoLUCENT™ PLUS-HRP reagents are…

  • Swift Film Cleaner™

    G-Biosciences

    Swift Film Cleaner™ allows researchers to clean film that has been overexposed or has a high background or speckling without having to repeat experiments. The Swift Film Cleaner™ is suitable for all experiments that use film to develop the result, including gel shift assays, Western,…

  • Superior ™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. Superior ™ Blocking Buffer is ideal for a high signal to background ratio in most…

  • Well-Coated ™ Protein L plates are designed to bind the kappa light chains of immunoglobulins without interfering with the antigen binding site. The Well-Coated ™ Protein L plates offer greater range of immunoglobulin classes and subclasses compared to Protein A , Protein G and…

  • Well-Coated ™ Neutravidin™ plates are designed to specifically bind biotinylated molecules, including biotin tagged antibodies, with minimal non-specific binding. This is particular advantageous for antibodies known to denature upon direct binding to polystyrene plates. Biotin…

  • Swift Western Diluent

    G-Biosciences

    The Swift™ Western Diluent is a unique solution that reduces the blocking and antibody incubations on Western blot membranes from >4 hours to <60 minutes. Swift™ Western Diluent generates comparable result to traditional Western blotting procedures and other commercial…

  • Well-Coated ™ Amine Binding plates are designed to specifically bind primary amines of peptides, proteins and other molecules. The Well-Coated ™ Amine Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other ligands for…

  • Western ReProbe™

    G-Biosciences

    Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse western blots. Western ReProbe™…

  • Well-Coated ™ Antibody plates are designed to specifically bind either mouse or rabbit IgG making them suitable for binding assays using low quantities of antibodies or antibodies that denature on direct binding to polystyrene plates. Another advantage of using the Well-Coated ™…

  • FirstChoice ™ Blocking Buffers contain a proprietary protein formulation that offers greater versatility and lack of cross-reactivity. This makes FirstChoice ™ Blocking Buffers ideal as a first choice for optimization of new assays, system or when determining the optimal blocking…

  • NAP-BLOCKER™

    G-Biosciences

    A major drawback of current blocking solutions, such as BSA , casein and milk powder, is that they are derived from animal sources. The presence of animal proteins can often lead to high non-specific backgrounds as antigens and antibodies, generated in animals, interact with the…

  • femtoCHROMO™-HRP Kit

    G-Biosciences

    femto CHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein band s with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X)…

  • FISH-Blocker

    G-Biosciences

    FISH-Blocker is a blocking agent that uses a fish protein as the primary blocking agent. The use of a fish protein, a non-mammalian protein, is that it eliminates or minimizes the interaction of antibodies raised in mammals. FISH-Blocker is one of the best blocking agents for immunoassays and it…

  • femtoELISA™

    G-Biosciences

    Enzyme-Linked Immunosorbent Assay (ELISA) is a useful, highly sensitive and powerful technique for the detection of proteins, chemicals, and drugs (antigens) in biological samples, including serum, blood and urine. The key to an ELISA is the interaction of a known antibody with the antigen of…

  • The femtoLUCENT™ PLUS-AP kit is based on alkaline phosphatase (AP) chemiluminescence reaction. The chemiluminescence light emission can be recorded by a short exposure to blue-light sensitive autoradiography films. The femtoLUCENT™ PLUS-AP kit allows for detection of femtogram quantity…

  • Well-Coated ™ Streptavidin plates are designed to specifically bind biotinylated molecules, including biotin tagged antibodies.This is particular advantageous for antibodies known to denature upon direct binding to polystyrene plates. Biotin exhibits an extraordinary binding affinity for…

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