NBT and BCIP refers to Nitro-Blue-Tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate respectively. When mixed together, BCIP is hydrolyzed and reacts with NBT, which then react with O2, forming visible purple precipitates in the presence of AP (alkaline phosphatase).The kit can be used for…

3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). In solution, TMB forms a blue product when allowed to react with peroxidase…

DAB, namely,3,3’-diaminobenzidine, as a chromogenic substrate of Peroxidase will lose electron, produce a gradually color change and form insouble dark blown matter when encountering H2O2. It can also be used for detection of activity of Peroxidase. When adding enhancer, the kit will have…