Western Blotting & ELISA

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Western Blotting & ELISA

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  • BLOK™ Casein

    G-Biosciences

    Convenient ready-to-use blocking solutions for Western blots, dot Blots & ELISA Features 1% Casein (w/v) solution for blocking Westerns, ELISA and dot Blots Ready-to-use Available in your choice of TBS or PBS buffers.

  • The best choice for routine, film-imaged Western blots WesternBright ECL is more sensitive than other chemiluminescent substrates, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. WesternBright ECL Spray provides both components of WesternBright…

  • HeLa Whole Cell Lysate, 500µg, Liquid

    Rockland Immunochemicals Inc

    Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes…

  • Incubation Trays

    Azure Biosystems

    Tray with clear top and bottom for staining and washing gels and membranes. Tray with opaque black top and bottom for staining and washing light sensitive gels and membranes.

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

  • Kit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody

  • HyBlot 26™ Blotting Paper, Dimensions: 14 x 17 in. (35.5 x 43cm), Qty: 100 0.18mm thickness Medium flow rate Compare to 1Chr Ideal for use in paper chromatography or as a gel dryer support. Also suitable for use as a spacer in blotting sandwiches

  • Superior ™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. Superior ™ Blocking Buffer is ideal for a high signal to background ratio in most…

  • 0.83mm thickness, smooth surface Economical - compare to GB003 Generally used as a blotter under the "weight" of a capillary sandwich. Assures continuous contact of buffer from the chamber through the gel and transfer membrane to the opposite pole (electroblot) or weight…

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

  • Western ReProbe PLUS

    G-Biosciences

    Based on our popular Western ReProbe™ , the modified formulation allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies…

  • Transform your protein standard into a chemiluminescent marker Write or draw on your transfer membranes with the WesternBright ChemiPen. With the proprietary "ink" you can make pre-stained protein standards chemiluminescent, annotate your blot with a date or blot number, or check…

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

  • Streptavidin Peroxidase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

  • NuSep’s Pre-cut blotting papers are ideal for use in transfer sandwiches and cassettes when proteins are transferred electrophoretically from a gel to a nitrocellulose, PVDF, or other membrane. Convenient – pre-cut; works with most mini gel transfer assemblies Tested –…

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

  • Well-Coated ™ Protein L plates are designed to bind the kappa light chains of immunoglobulins without interfering with the antigen binding site. The Well-Coated ™ Protein L plates offer greater range of immunoglobulin classes and subclasses compared to Protein A , Protein G and…

  • Well-Coated™ Nickel plates are designed to specifically bind 6X histidine (polyhistidine) tagged proteins and peptides. The plates can be used for the isolation of polyhistidine-tagged proteins direct from bacterial lysates and then used for subsequent ELISA protocols. The wells are…

  • Fluorescent streptavidin conjugates to detect biotinylated proteins These streptavidin conjugates are prepared from phycobiliproteins, light-harvesting components of the photosynthetic apparatus found in certain types of algae and cyanobacteria. Phycobiliproteins harvest the light very…

  • picoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. picoLUCENT™ PLUS-HRP reagents are…

  • Blot Boxes

    MTC Bio

    Minimize antibody usage Hinged lid reduces evaporation For halves, wholes, strips, or minis Whether you're doing Western blots in halves, wholes, strips or minis, we have the right Western blot box for you. Choose the size that suits your needs and you'll be able to instantly reduce…

  • Black plastic hinged box ideal for the incubation, blocking, and washing steps for Western Blotting using Fluorescent Conjugated Antibodies such as DyLight Conjugated Secondary Antibodies or IRDye Antibody Conjugates. This large box is best for volumes between 10mL and 30mL.

  • Enhanced protein transfer for improved sensitivity Achieve improved detection of low-abundance and posttranslationally modified proteins with Advansta's proprietary FLASHBlot Transfer Buffer. High-efficiency protein transfer and increased protein retention on the membrane add up to more…

  • 0.40mm thickness Compare to 3Chr Commonly used as wicks in protein or nucleic acid blotting. Provides uniform buffer flow through gel to transfer membrane.

  • 0.40mm thickness Compare to 3Chr Commonly used as wicks in protein or nucleic acid blotting. Provides uniform buffer flow through gel to transfer membrane.

  • Blocking Solution

    Quality Biological

    Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Reduce nonspecific binding Applications Quality Biological’s Blocking Solution is prepared from non-fat dried milk in PBS containing sodium azide 0.02% (w/v) as a…

  • 0.18mm thickness Medium flow rate Compare to 1Chr Ideal for use in paper chromatography or as a gel dryer support. Also suitable for use as a spacer in blotting sandwiches

  • Enhancer™ allows for the rapid application of up to 384 protein or nucleic acid samples on to a 12 x 9cm membrane for subsequent analysis. Samples are applied to a nitrocellulose or nylon membrane by capillary action, resulting in concentration of sample due to minimal diffusion. …

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is manufactured…

  • Pre-mixed, standard Laemmli loading buffers for protein electrophoresis These ready-to-use Laemmli loading buffers contain Bromophenol blue to enable visualization of the dye front and monitoring of the progress of electrophoresis. Choose from reducing or non-reducing buffers, each supplied…

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