Western Blotting & ELISA

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Western Blotting & ELISA

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  • Well-Coated ™ Amine Binding plates are designed to specifically bind primary amines of peptides, proteins and other molecules. The Well-Coated ™ Amine Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other ligands for…

  • NBT/BCIP Stain Kit

    Bio Basic Inc.

    NBT and BCIP refers to Nitro-Blue-Tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate respectively. When mixed together, BCIP is hydrolyzed and reacts with NBT, which then react with O2, forming visible purple precipitates in the presence of AP (alkaline phosphatase).The kit can be used for…

  • Kit contains materials for 10 Western blots – LF PVDF membranes, wash buffer, fluorescent blocking buffer, secondary antibodies AzureSpectra ms700/rb800

  • BLOK™ BSA

    G-Biosciences

    Protein solutions are used for blocking excess binding sites in ELISA, blotting, immunohistochemistry and other immunochemical applications. Each blocking solution will impart certain characteristics on the assay system. Our BLOK™ BSA is a 10% solution of BSA (Bovine Serum Albumin) …

  • 0.40mm thickness Compare to 3Chr Commonly used as wicks in protein or nucleic acid blotting. Provides uniform buffer flow through gel to transfer membrane.

  • Blotting Paper, Grade 238

    Ahlstrom-Munksjo

    Ahlstrom manufactures a complete line of high quality chromatography, electrophoresis and blotting papers for use in gel transfer applications and chromatography techniques. These uniform, smooth, white sheets are the high purity, high quality, and high value standard. They are used in nucleic acid…

  • Pre-cut LF PVDF membrane (13x18 cm vertical gels); 10 sheets

  • 0.40mm thickness Compare to 3Chr Commonly used as wicks in protein or nucleic acid blotting. Provides uniform buffer flow through gel to transfer membrane.

  • 0.8 mm general purpose blotting paper recommended for the lysis/denaturation of colony or plaque lifts as well as Western blots. Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer…

  • 0.18mm thickness Medium flow rate Compare to 1Chr Ideal for use in paper chromatography or as a gel dryer support. Also suitable for use as a spacer in blotting sandwiches

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

  • High sensitivity X-ray film for chemiluminescent Western blots LucentBlue is a sensitive film ideal for imaging chemiluminescent Western blots. LucentBlue is guaranteed to perform with WesternBright™ ECL, WesternBright Quantum and WesternBright Sirius. LucentBlue is compatible with…

  • 2.45mm thickness, replaces paper towels Completes blotting "sandwich" Smoother, more uniform texture

  • Blotting Papers

    Advansta

    Advansta's pre-cut Blotting Papers are ideal for use in transfer sandwiches and cassettes when proteins are transferred electrophoretically from a gel to a nitrocellulose, PVDF, or other membrane. The Advansta Blotting Papers are available in two sizes to work with multiple transfer setups and…

  • 0.40mm thickness Compare to 3Chr Commonly used as wicks in protein or nucleic acid blotting. Provides uniform buffer flow through gel to transfer membrane.

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

  • femtoCHROMO™-AP Kit

    G-Biosciences

    femto CHROMO™ AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X)…

  • Incubation Trays

    Azure Biosystems

    Tray with clear top and bottom for staining and washing gels and membranes. Tray with opaque black top and bottom for staining and washing light sensitive gels and membranes.

  • 0.18mm thickness Medium flow rate Compare to 1Chr Ideal for use in paper chromatography or as a gel dryer support. Also suitable for use as a spacer in blotting sandwiches

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

  • NAP-BLOCKER™

    G-Biosciences

    A major drawback of current blocking solutions, such as BSA , casein and milk powder, is that they are derived from animal sources. The presence of animal proteins can often lead to high non-specific backgrounds as antigens and antibodies, generated in animals, interact with the…

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is manufactured and…

  • Well-Coated ™ Protein G plates are designed to bind the constant (Fc) region of immunoglobulins ensuring that the antigen binding domain of the antibody is orientated away from the plate, offering maximum exposure of the binding site.The immunoglobulin orientation on the Well-Coated ™…

  • SWIFT™ Transfer Pads

    G-Biosciences

    Western blot analysis of proteins is a routine and commonly used technique in research laboratories, with 3 major drawbacks. The first is the amount of time taken to transfer the proteins to a protein binding membrane; the second is the variable efficiency of the transfer and the third is problems…

  • The best choice for routine, film-imaged Western blots WesternBright ECL is more sensitive than other chemiluminescent substrates, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. Save time and money with WesternBright ECL. Features …

  • Immobilon NOW Dispenser

    MilliporeSigma

    Dispenser for transfer membrane used in Western blotting, immunoblotting and dot blotting applications The Immobilon NOW Dispenser is ideal for safe storage and convenient use of 8.5 cm wide rolls of transfer membrane for Western blotting. With this device, membrane can be efficiently cut to…

  • Swift Film Cleaner™

    G-Biosciences

    Swift Film Cleaner™ allows researchers to clean film that has been overexposed or has a high background or speckling without having to repeat experiments. The Swift Film Cleaner™ is suitable for all experiments that use film to develop the result, including gel shift assays, Western,…

  • Blotting Paper, Grade 222

    Ahlstrom-Munksjo

    This medium thick paper offers extremely high flow and is recommended for both electrophoresis of large molecules and as a blotter to complete the blotting sandwich in gel transfer applications. The smooth and uniform texture assures continuous contact of buffer from the chamber through the gel and…

  • 0.83mm thickness, smooth surface Economical - compare to GB003 Generally used as a blotter under the "weight" of a capillary sandwich. Assures continuous contact of buffer from the chamber through the gel and transfer membrane to the opposite pole (electroblot) or weight…

  • The best choice for routine, film-imaged Western blots WesternBright ECL is more sensitive than other chemiluminescent substrates, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. WesternBright ECL Spray provides both components of WesternBright…

  • 100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

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