Western Blotting & ELISA

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Western Blotting & ELISA

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  • Blotting Papers, Grade GB003

    GE Healthcare

    0.8 mm general purpose blotting paper recommended for the lysis/denaturation of colony or plaque lifts as well as Western blots. Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer…

  • Blocking Solution

    Quality Biological

    Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Reduce nonspecific binding Applications Quality Biological’s Blocking Solution is prepared from non-fat dried milk in PBS containing sodium azide 0.02% (w/v) as a…

  • 10X Fish Gelatin Blocking Agent

    Biotium

    10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum…

  • Well-Coated™ Amine Binding Plates

    G-Biosciences

    Well-Coated ™ Amine Binding plates are designed to specifically bind primary amines of peptides, proteins and other molecules. The Well-Coated ™ Amine Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other ligands for…

  • GB003 110x140MM 100/PK

    GE Healthcare

  • GB003 70x100MM 100/PK

    GE Healthcare

  • WesternBright™ Transfer Membranes

    Advansta

    Convenient Nitrocellulose and PVDF membranes for Western blotting Advansta offers a large selection of nitrocellulose and polyvinylidene difluoride (PVDF) transfer membranes for immunoblotting applications. Choose from convenient pre-cut membranes sized for protein minigels and economical…

  • PBST Buffer 10x Strength Solution

    Bio Basic Inc.

  • Blotting Papers, Grade GB005

    GE Healthcare

    1.5 mm highly absorbent paper recommended for semi-dry protein blotting Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps. Manufactured and tested specifically for…

  • HyBlot 30™ Blotting Paper, Dimensions: 14 x 17 inch (gel dryer size), Qty: 50

    Denville

    2.45mm thickness, replaces paper towels Completes blotting "sandwich" Smoother, more uniform texture

  • Grade 3MM Chr Cellulose Chromatography Papers

    GE Healthcare

    3MM Chr cellulose blotting sheets Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps. Manufactured and tested specifically for chromatographic and blotting techniques.…

  • Well-Coated™ Nickel Plates

    G-Biosciences

    Well-Coated™ Nickel plates are designed to specifically bind 6X histidine (polyhistidine) tagged proteins and peptides. The plates can be used for the isolation of polyhistidine-tagged proteins direct from bacterial lysates and then used for subsequent ELISA protocols. The wells are…

  • Nitrocellulose Membranes

    G-Biosciences

    For user's convenience, we offer pre-cut transfer membranes and padding for Western blot transfer procedures. Pre-cut membranes are supplied sandwiched between blotting paper padding. Simply soak the membrane in transfer buffer and assemble with the gel in a transfer cassette. …

  • GB003 200x250MM 100/PK

    GE Healthcare

  • Swift™ Western Blotting System

    G-Biosciences

    The Swift™ Western Blotting System is a unique system that reduces the blocking and antibody incubations on Western blot membranes from >4 hours to <60 minutes. Using a combination of proprietary wash and diluent buffers and our highly sensitive femtoLUCENT™ …

  • PVDF Membranes

    G-Biosciences

    Our PVDF (polyvinylidene) membranes are pre-cut membranes for Western transfers, or Western blotting.The PVDF membranes bind biomolecules, including proteins, through hydrophobic interactions The presence of the 0.45µm pore sizes increases the surface binding area. Typical…

  • HyBlot 3A™ Gel Blot Paper, Dimensions: 10 x 15cm, Qty: 100

    Denville

    100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

  • Transfer Membranes

    NuSep

    NuSep offers a large selection of nitrocellulose (NC) and polyvinylidene difluoride (PVDF) transfer membranes for immunoblotting applications. Choose from convenient pre-cut membranes sized for protein minigels and economical membrane rolls. All membranes are optimized for compatibility with a…

  • GB003 100x150MM 100/PK

    GE Healthcare

  • Protein-Free Blocking Buffer

    G-Biosciences

    Protein-Free Blocking Buffer does not contain protein; it is a proprietary formation of non-protein agents that eliminates non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free Blocking Buffer eliminates problems…

  • Blotting Papers

    Advansta

    Advansta's pre-cut Blotting Papers are ideal for use in transfer sandwiches and cassettes when proteins are transferred electrophoretically from a gel to a nitrocellulose, PVDF, or other membrane. The Advansta Blotting Papers are available in two sizes to work with multiple transfer setups and…

  • HyBlot 30™ Blotting Paper, Dimensions: 20 x 20 CM (gel dryer size), Qty: 50

    Denville

    2.45mm thickness, replaces paper towels Completes blotting "sandwich" Smoother, more uniform texture

  • Avant™ Buffer Pouches

    Advansta

    Convenient, pure, fast buffer preparation Advansta's Avant buffer pouches are perfect for standardizing your electrophoresis and Western blotting applications. Dissolve a pouch of pre-measured molecular biology-grade chemicals in 500mL of deionized water to obtain a ready-to-use 1x…

  • WesternOne™ Marker Pen

    ACTGene, Inc.

    HRP Pen for Western Blot Chemiluminescent Detection Add Magic Touch to your Western Blot and Transform Membrane in Seconds Perfect tool for chemiluminescent detection in Western blot. It can be easily used to write/draw on your transfer membranes and make pre-stained marker…

  • WesternBright™ ChemiPen™

    Advansta

    Transform your protein standard into a chemiluminescent marker Write or draw on your transfer membranes with the WesternBright ChemiPen. With the proprietary "ink" you can make pre-stained protein standards chemiluminescent, annotate your blot with a date or blot number, or check…

  • TMB Stain Kit

    Bio Basic Inc.

    3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). In solution, TMB forms a blue product when allowed to react with peroxidase…

  • ELISABright™ Chemiluminescent Substrate

    Advansta

    Chemiluminescent substrate for ELISA applications ELISABright is an enhanced and highly sensitive chemiluminescent HRP substrate optimized for chemiluminescent ELISA applications. This luminol-based substrate demonstrates a wide linear dynamic range and a superior signal to noise ratio.…

  • HyBlot 3A™ Gel Blot Paper, Dimensions: 7 x 10cm, Qty: 100

    Denville

    100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

  • OptiBlaze™ ELISA

    G-Biosciences

    Stabilized ultra sensitive luminol and 1,2 dioxetane based horseradish peroxidase or alkaline phosphatase substrate for the detection of HRP or AP-conjugated antibodies during ELISA. OptiBlaze™ ELISA femto-HRP or OptiBlaze™ ELISA femto-AP are chemiluminescent detection systems for…

  • Background Quenching Sheets

    Advansta

    For improved imaging of chemiluminescent blots and fluorescent blots and gels Use Advansta's Background Quenching Sheets to obtain the best images of chemiluminescent Western blots and fluorescent blots and gels. For Chemiluminescence Placed under chemiluminescent blots…

  • VisiGlo PLUS™ HRP Chemiluminescent Substrate Kit

    Amresco

    VisiGlo™ HRP and VisiGlo PLUS™ HRP are luminol-based chemiluminescent substrates designed for the rapid and sensitive detection of peroxidase labeled-conjugates. They offer increased sensitivity compared to chromogenic substrates in both blotting and ELISA applications. VisiGlo™ HRP and VisiGlo …

  • AdvanWash™ Washing Solution

    Advansta

    Washing solutions optimized for use with WesternBright systems AdvanWash washing solution is optimized to work with Advansta Western blotting system. Choose AdvanWash for all WesternBright chemiluminescent HRP substrates and the WesternBright MCF fluorescent Western blotting kit.…

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