Substrates

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  • 5-Bromo-4-Chloro-3-Indolyl-ß-D-Galactopyranoside

    MP Biomedicals

    5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate. X-Gal is particularly useful in molecular biology applications to detect the activity of…

  • Glutathione, Reduced

    MP Biomedicals

    Glutathione is the major low molecular weight thiol compound of the living plant or animal cell. It is a tripeptide with a gamma peptide linkage between the amine group of cysteine (which is attached by normal peptide linkage to a glycine) and the carboxyl group of the glutamate side-chain. It is…

  • Salicin

    MP Biomedicals

    Salicin is a substrate for β-glucosidase.

  • Sodium Azide

    MP Biomedicals

    Sodium azide is used as a preservative for laboratory reagents; a bacteriostatic agent in storage solutions. Inhibits gram negative flora so is therefore included in culture media for the selective isolation of Streptococci and Staphylococci. It has been used to avoid bacterial contamination during…

  • o-Nitrophenyl-ß-D-Galactopyranoside

    MP Biomedicals

    o-Nitrophenyl-β-D-galactoside (ONPG) is a colorimetric substrate for b-galactosidase. This product is hydrolyzed to o-nitrophenol by the enzyme. o-Nitrophenol is yellow in alkaline solution and can be quantitated at 410-420 nm 2 . o-Nitrophenyl-β-D-galactoside is a cell culture…

  • 5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt

    MP Biomedicals

    A chromogenic substrate for alkaline phosphatase. For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of…

  • Leupeptin Hydrochloride

    MP Biomedicals

    A modified tripeptide reversible protease inhibitor of trypsin-like proteases and some cysteine proteases including endoproteinase Lys-C, kallikrein, papain, thrombin, cathepsin B, cathepsins H and L, trypsin, calpain, trypsin and plasmin. Little to no inhibition is seen against pepsin, cathepsins…

  • 2,2'-Azino-Bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) Diammonium Salt

    MP Biomedicals

    2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl…

  • BCIP/NBT Liquid Substrate Plus

    MP Biomedicals

    BCIP/NBT Liquid Substrate Plus is a stabilized chromogen solution. It has a greater sensitivity and produces little or no background staining compared with other alternatives. It is a ready to use solution. The BCIP/NBT Liquid Substrate System offers a convenient, ready-to-use substrate solution…

  • 3,3',5,5'-Tetramethylbenzidine

    MP Biomedicals

    (TMB Powder) This product is excellent for ELISA procedures. C16H20N2 MW 240.3

  • Gly-Phe-7-Amino-4-Trifluoromethylcoumarin

    MP Biomedicals

    Gly-Phe-AFC is a fluorogenic, cell-permeant peptide substrate, used in Cytotoxicity testing. In vitro cytotoxicity testing has become an integral aspect of drug discovery because it is a convenient, costeffective, and predictive means of characterizing the toxic potential of new chemical entities. …

  • N-Formylmethionine Specific Transfer Ribonucleic Acid from E. coli MRE 600

    MP Biomedicals

    Each T-RNA molecule is specific for and binds with a particular amino acid; more than one exists for each amino acid. T-RNA performs three functions during protein synthesis: binds with its specific amino acid, recognizes the corresponding codon on mRNA and places the amino acid in the correct…

  • Suc-Leu-Leu-Val-Tyr-AMC

    MP Biomedicals

    Fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S proteasome, calpains and other chymotrypsin-like proteases. Extinction: 380nm. Emission: 460 nm. This substrate is useful for inhibitor screening and kinetic analysis.

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