HOOK™-PEG₂-Iodoacetyl-Biotin reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups.…

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹â�µM-¹) and streptavidin (Ka=10¹â�µM-¹). Biotin and (strept)avidin interaction is rapid. Once the bond is established, it can survive up to 3M…

HOOK™-NHS-SS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are…

HOOK™-Biotin-PEG₃-Amine reacts with protein carboxyl groups in the presence of the heterobifunctional cross-linker EDC. HOOK™-Biotin-Pentylamine, HOOK™-Biotin-PEG₂-Amine and HOOK™-Biotin-PEG₃-Amine are water soluble, due to its polyethylene…

HOOK™-Sulfo-NHS-LC-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to…

HOOK™-Iodoacetyl-LC-Biotin reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free…

HOOK™-NHS-dPEG™₄-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of…

HOOK™-Biotin-PEG₂-Amine reacts with protein carboxyl groups in the presence of the heterobifunctional cross-linker EDC. HOOK™-Biotin-Pentylamine, HOOK™-Biotin-PEG₂-Amine and HOOK™-Biotin-PEG₃-Amine are water soluble, due to its polyethylene…

HOOK™-Biotin-Hydrazide reacts with oxidized carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself but conjugate to the carbohydrate residues of glycoproteins. Carbodhydrate reactive biotin reagents contain hydrazides (-NH-NH₂) as a reactive…

HOOK™-PFP-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are…

HOOK™-Sulfo-NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…

HOOK™-Sulfo-NHS-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…

HOOK™-Biotin-BMMCC reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhydryl…

HOOK™ NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are…

HOOK™-NHS-LC-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…

HOOK™-Biotin-PDA reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhydryl…

HOOK™-Sulfo-NHS-SS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…

HOOK™-Biotin-LC-Hydrazide reacts with oxidized carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself but conjugate to the carbohydrate residues of glycoproteins. Carbodhydrate reactive biotin reagents contain hydrazides (-NH-NH₂) as a reactive…

HOOK™-NHS-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are…