Separopore Bioaffinity Matrices

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Separopore Bioaffinity Matrices

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  • Separopore® (Agarose) 4B

    bioWORLD

    Separopore® 4B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 4B is a proven base matrix for coupling affinity ligands. Separopore® 4B provides good…

  • NAD-Separopore® (Agarose) 4B-CL (Lyophilized)

    NAD covalently bound to Separopore® has a high affinity for NAD-dependent enzymes and dehydrogenases. NAD-Separopore® 4B-CL has been used to purify Vibrio cholerae toxin, diptheria toxin and other NAD binding proteins.

  • EAH Separopore® (Agarose) 4B

    EAH-Separopore® 4B is used to couple molecules containing carboxyl groups to the matrix for affinity chromatography. It was prepared by covalent coupling of 1, 6-diamino hexane to Separopore® 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing…

  • Anti-Bovine Albumin BSA Separopore®

    bioWORLD

    Application: Anti-BSA-Separopore® 4B is used for immunoprecipitation and immunoaffinity purification of bovine serum albumin (BSA) from solutions (and co-immunoprecipitation of biomolecules that bind BSA). It is also used to remove BSA from solutions.

  • Anti-GFP Tag (GF28R), Monoclonal Ab

    bioWORLD

    Anti-GFP Tag (GF28R) mAb recognizes both the native and denatured forms of GFP (Green Fluorescent Protein) and its many popular variants: EGFP, YFP, EYFP, and CFP. Description: Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody Clonality: GF28R Host Species: Mouse …

  • IgG (Rabbit) Separopore® (Agarose) 4B

    Used for purification of anti-IgG antibodies or to purify Protein A. An affinity chromatography gel matrix consisting of rabbit IgG bound to 4% agarose beads (Separopore® 4B). This matrix is coupled through a stable, leak resistant linkage over a wide pH range. May also be used to immunoprecipitate…

  • Separopore® (Agarose) 6B

    bioWORLD

    Applications: Separopore® 6B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 6B is a proven base matrix for coupling affinity ligands. Separopore® 6B…

  • Lactose Separopore® (Agarose)6B-CL

    a-Lactose-Separopore® 4B-CL is used in protein chromatography, affinity chromatography and carbohydrate matrices. a-Lactose-Separopore® 4B-CL has been used to study microneme proteins and host-parasite cell surface receptors. Lactose-Separopore® 4B-CL may be used to isolate lactose binding…

  • NADP-Separopore® (Agarose) 4B-CL (Lyophilized)

    NADP covalently bound to Separopore® has a high affinity for NADP-dependent enzymes and dehydrogenases. Acetyl-CoA binds to NADP-Separopore® 4B-CL twelve times higher than NAD-Separopore® 4B-CL. NADP-Separopore® 4B-CL has also been used to purify extracellular nucleases.

  • Galactose Separopore® Agarose 4B-CL

    Galactose coupled Separopore® is a high strength bioagarose matrix. Galactose-Separopore® 4B-CL is used in the purification of agglutinins, toxins, lectins, galactosidases and other galactose-binding molecules, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins. This…

  • IDA Chelating Separopore® (Agarose) 4B-CL

    Metal ion-chelating Separopore® 4B-CL is designed for the affinity purification of 6X Histidine-tagged proteins. Nickel, Cobalt, Copper and Zinc are used as cations for this type of purification because they provide effective binding and selectivity for a wide range of proteins. Affinity matrix for…

  • DEAE Separopore® (Agarose) 6B-CL

    DEAE-Separopore® 6B-CL is a weak anion exchanger with excellent flow capacities and a high capacity for binding and resolution of proteins of all pI values. bio-World's DEAE-Separopore® 6B-CL was prepared by covalent attachment of diethylaminoethyl (DEAE) to Separopore® 6B-CL. DEAE remains charged…

  • Separopore® (Agarose) 2B

    bioWORLD

    Applications: Separopore® 2B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for for both analytical and preparative protein purifications. Separopore® 2B is a proven base matrix for coupling affinity ligands. Separopore® 2B provides a good…

  • Glutathione Separopore® Microbeads

    bioWORLD

    Applications: Reduced glutathione is covalently linked Separopore® 4B Microbeads for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…

  • Cu-Chelated Separopore® Agarose

    bioWORLD

    Cu-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Cu metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Cu metal ions.v…

  • Glutathione Separopore® (Agarose) 4B

    bioWORLD

    Applications: Reduced glutathione is covalently linked Separopore® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…

  • Separopore® (Agarose) 6B-CL

    bioWORLD

    Separopore® 6B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore® 6B-CL is a proven base matrix for coupling affinity ligands. Separopore® 6B-CL…

  • Guar gum Separopore® (Agarose)4B-CL

    bioWORLD

    Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

  • CDI-Activated Separopore® Agarose 4B

    Pre-activated medium for immobilization of ligands containing primary amines N, N'-Carbonyl diimidazole (CDI) mediated activation of Separopore® 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation No charge is introduced into the matrix after the coupling…

  • Separopore® (Agarose) 6B-MB

    bioWORLD

    Separopore® 6B (macrobeads) is a larger sized agarose macrobead matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Larger volumes available at discounted prices (enquire).

  • Gelatin Separopore® (Agarose) 4B

    Gelatin Separopore® 4B has been designed for the purification or removal of fibronectin. Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein found on the surfaces of many cell types and present in many extracellular fluids including plasma.

  • Aldehyde Separopore® (Agarose) 4B-CL

    bioWORLD

    Applications: Aldehyde Separopore® 4B has a robust reductive amino alkylation coupling chemistry to immobilize proteins and other ligands through primary amines (–NH2) to the resin. It can be used for a variety of affinity purification methods involving batch or column chromatography.…

  • Protein-A Blue Separopore® Ag 4B-MB

    bioWORLD

    Applications: Protein A Blue-Separopore® 4B-MB functions same as conventional Protein A affinity beads, but offers enhanced visibility due to blue colored Separopore® macrobeads. The blue colored beads help provide visual monitoring to avoid the accidental loss of beads during pull-down in…

  • Calmodulin Separopore® (Agarose)

    Calmodulin-Separopore® 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with…

  • Zn-Chelated Separopore® Agarose

    bioWORLD

    Zn-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Zn metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Zu metal…

  • Epoxy-activated Separopore® (Agarose) 4B-CL

    Used in coupling of hydroxyl, amino or thiol groups to Separopore® 4B via a 12-carbon hydrophilic spacer arm Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group The matrix provides a 10-atom spacer arm convenient for coupling of…

  • 2-Iminobiotin Separopore® Ag. 6B

    bioWORLD

    Applications: 2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from…

  • Ubiquitin Separopore® (Agarose)

    bioWORLD

    Applications: Ubiquitin-Separopore® 4B is useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases. Ubiquitin-Separopore® 4B is used in affinity chromatography to study enzymes involved in…

  • Papain Separopore® (Agarose) 4B

    Papain-Separopore® 4B can be used as substitute of free papain in many applications. Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. This matrix is used to produce Fab fragment of IgG antibodies. It is also…

  • Blue-Separopore® (Agarose) 6B-CL

    Cibacron Blue 3GA-Separopore® 6B-CL is an agarose conjugate in saline suspension used to searate and purify a variety of proteins. The ligand Cibacron Blue F3G-A is covalently coupled to Separopore® via chlorotriazine ring. The structure of the blue dye makes it a very versatile tool for separating…

  • KLH Separopore® (Agarose) 4B

    Affinity matrix with immobilized KLH used for the purification of antibodies generated against peptide-KLH conjugated antigens byremoving antibodies against the KLH portion of the antigen. Anti-KLH antibodies can be removed by solid phase immuno-affinity column chromatography over the KLH-…

  • Separopore® (Agarose) 2B-CL

    bioWORLD

    Applications: Separopore® 2B-CL is a cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone of Separopore® 2B-CL results in low non-specific interactions and high protein recoveries. Separopore® 2B-CL provides an excellent separation of proteins…

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