LGC, Biosearch Technologies Chemicals Reagents P
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NxGen T7 RNA Polymerase
LGC, Biosearch TechnologiesT7 RNA Polymerase catalyzes the 5'′→3' RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity.
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MasterPure Gram Positive DNA Purification Kit 100 Purif.
LGC, Biosearch TechnologiesThe MasterPure™ Gram-Positive DNA Purification Kit provides all of the reagents needed to purify DNA from Gram-positive bacteria.
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MMLV REVERSE TRANS 1ST-STRD CDNA SYN KIT 50 rxns
LGC, Biosearch TechnologiesThe MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit is optimized for generating full-length first-strand cDNA from total RNA preparations or purified Poly(A)+ RNA.
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Durascribe T7 Transcription Kits
LGC, Biosearch TechnologiesThe DuraScribe® T7 Transcription Kit produces 2'-Fluorine-modified RNA transcripts—called DuraScript® RNA—that are completely resistant to RNase A. Ideal for SELEX RNA-aptamer selection procedures.
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2'-dNTP (All 4 Mixed) 10 µmole
LGC, Biosearch TechnologiesSterile, neutral pH solutions of dATP, dCTP, dGTP, and dTTP are available either as individual nucleotides or a mixture of all four nucleotides for use in DNA synthesis.
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E. cloni 10G BAC-Optimized Electrocomp Cell 25 rxns
LGC, Biosearch TechnologiesE. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 10^7 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning. Manufactured using a novel procedure that maximizes transformation efficiencies with very large…
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Endura Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be…
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RNA 5' Polyphosphatase 200 U
LGC, Biosearch TechnologiesRNA 5´ Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the ? and ß phosphates from 5´-triphosphorylated RNA (such as primary RNA transcripts): 5´ pppN—OH 3´ ′→ 5´ pN—OH 3´ + 2 Pi
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RapiDxFire Hot Start Taq DNA Polymerase
LGC, Biosearch TechnologiesRapiDxFire™ Hot Start Taq DNA Polymerase is recombinant Taq DNA Polymerase from Thermus aquaticus bound to select blockers, which prevents unwanted, nonspecific amplification during reaction set up at temperatures less than 60 °C. Enzyme activity is restored after a short 15 second burst…
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Proteinase K 2 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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RNase A 2 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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QuickExtract Plant DNA Extraction Solution 50 ml
LGC, Biosearch TechnologiesThe QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes.
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TRANSFORMAX EPI300-T1R ELECTRO E. COLI 20 rxns
LGC, Biosearch TechnologiesPhage T1-resistant electrocompetent cells specially engineered to regulate the copy number of CopyControl Fosmid clones.
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Exonuclease I 20,000 U
LGC, Biosearch TechnologiesExonuclease I digests ssDNA in a 3´′→5´ direction, but does not digest dsDNA. Although it requires the presence of magnesium and a free 3´-hydroxyl terminus for activity, it is active under a wide variety of buffer conditions and can be added directly into most reaction mixes.…
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MasterAmp Tth DNA Polymerase 250 U
LGC, Biosearch TechnologiesMasterAmp™ Tth DNA Polymerase is a recombinant thermostable DNA polymerase for use in PCR. This enzyme has optimal DNA synthetic activity at temperatures above 70°C and can be used at temperatures up to 95°C.
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QuickExtract RNA Extraction Kit 50 ml
LGC, Biosearch TechnologiesThe QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis.
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Copycontrol Induction Solution 25 Ml
LGC, Biosearch TechnologiesPrepare unbiased fosmid libraries of approx. 40 kb that can be maintained at single-copy number and induced to high-copy number as needed when grown in the TransforMax EC300 E. coli.
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RNase I 1,000 U
LGC, Biosearch TechnologiesRNase I degrades single-stranded RNA to nucleoside 3´ monophosphates via 2´,3´ cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3 unlike RNase A, which cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70°C…
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COPYCUTTER INDUCTION SOLUTION 25 ml
LGC, Biosearch TechnologiesPrepare unbiased fosmid libraries of approx. 40 kb that can be maintained at single-copy number and induced to high-copy number as needed when grown in the TransforMax EC300 E. coli.
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RIBONUCL-5'-TRIPHOSPHATE (NTP) SOL 25 umole ea
LGC, Biosearch TechnologiesOne tube each of ATP, CTP, GTP and UTP at 100 mM. Each is provided as a sterile, neutral solution.
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Exo-Minus Klenow DNA Polymerase 1,000 U
LGC, Biosearch TechnologiesExo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´′→3´ and 3´′→5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I has two mutations (D355A and E357A).
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LavaLAMP™ RNA Component Kits
LGC, Biosearch TechnologiesThe LavaLAMP™ RNA Component Kit is a deconstruction of the LavaLAMP™ RNA Master Mix, and as such, when reactions are setup using the initial Experimental reaction conditions outlined in the Component Kit User Manual, the composition of that reaction matches the composition of the…
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ER2738 Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. ER2738 Electrocompetent Cells are an amber suppressor strain (glnV) prepared as highly efficient (=2 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New…
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Plasmid-Safe ATP-Dependent DNase
LGC, Biosearch TechnologiesPlasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.
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EpiScript RNase H- Reverse Transcriptase
LGC, Biosearch TechnologiesEpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity
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EZ-TN5 TRANSPOSASE 10 U
LGC, Biosearch TechnologiesEZ-Tn5™ Transposase is a hyperactive Tn5 transposase for random insertion of an EZ-Tn5 Transposon into any cloned DNA in vitro or for preparing an EZ-Tn5 Transposome for random insertion of a EZ-Tn5 Transposon into the genomic DNA of living bacteria.
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OverExpress Chem Comp Cells
LGC, Biosearch TechnologiesLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins…
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Rec J Exonuclease 250 U
LGC, Biosearch TechnologiesRec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´′→3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.
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TE Buffer 70 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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Green Fluorescent Dye 500 rxns
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Component Kit with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.
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E. cloni 10G CLASSIC Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…