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  • NxGen T4 DNA Ligase

    LGC, Biosearch Technologies

    T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

  • Sterile Nuclease-Free Water 50 ml

    LGC, Biosearch Technologies

    Epicentre's Molecular Biology Grade Nuclease-Free Water is free of DNA exo- and endonuclease activities, and RNase activity.

  • Ampligase DNA Ligase

    LGC, Biosearch Technologies

    Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures

  • Exonuclease III 25,000 U @ 200 U/µl

    LGC, Biosearch Technologies

    Exonuclease III digests duplex DNA in a 3´′→5´ direction from a nick, a blunt end, or 3´-recessed end, producing stretches of ssDNA on the opposite strand

  • Expresso Rhamnose Cloning & Exp Systems

    LGC, Biosearch Technologies

    The Expresso Rhamnose Cloning and Protein Expression Systems are designed for fast, easy, and efficient directional cloning and expression of PCR-amplified genes using Expressioneering Technology. Expressioneering Technology uses in vivo homologous recombination to seamlessly clone PCR amplified…

  • COPYCUTTER INDUCTION SOLUTION 25 ml

    LGC, Biosearch Technologies

    Prepare unbiased fosmid libraries of approx. 40 kb that can be maintained at single-copy number and induced to high-copy number as needed when grown in the TransforMax EC300 E. coli.

  • CloneID 1X Colony PCR Master Mix

    LGC, Biosearch Technologies

    CloneID™ 1X Colony PCR Master Mix a PCR enzyme specifically formulated for Colony PCR, is provided in a 1X Master Mix format that withstands 98°C denaturation. All required buffer components, enzyme, Mg++ and dNTP’s are all present in optimized concentrations. The product also contains…

  • RNase I 1,000 U

    LGC, Biosearch Technologies

    RNase I degrades single-stranded RNA to nucleoside 3´ monophosphates via 2´,3´ cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3 unlike RNase A, which cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70°C…

  • Endura Chemically Competent Cells

    LGC, Biosearch Technologies

    Lucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be…

  • Episcript Rnase H- Reverse Transcriptase

    LGC, Biosearch Technologies

    EpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity

  • MasterAmp PCR Optimization Kit

    LGC, Biosearch Technologies

    The MasterAmp™ PCR Optimization Kits offer simplified PCR optimization with a thermostable DNA polymerase of choice, PCR of difficult or high-GC templates and PCR amplifications up to 20 kb. For rapid, dependable optimization of PCR, we encourage you to try the FailSafe™ PCR System to…

  • Bst DNA Polymerase

    LGC, Biosearch Technologies

    Bst DNA Polymerase, Exonuclease Minus, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5’-3’ polymerase activity and strand displacement activity, but it lacks 3’-5’ exonuclease activity. It also has reverse transcription…

  • QuickExtract DNA Extraction Solution

    LGC, Biosearch Technologies

    The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.

  • RapiDxFire qPCR 5X Master Mix GF

    LGC, Biosearch Technologies

    RapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X…

  • 2.5 mM dNTP Mix, PCR Grade

    LGC, Biosearch Technologies

    This ready-to-use dNTP Mix is a mixture of all four nucleotides (dATP, dCTP, dGTP, dTTP), each dissolved in highly purified water at a concentration of 2.5 mM, at pH 8.3. Two milliliters of the Mix is enough for 500 standard 50 µl EconoTaq® Polymerase PCR reactions.

  • QuickExtract RNA Extraction Kit 50 ml

    LGC, Biosearch Technologies

    The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis.

  • NxSeq AmpFREE Low DNA Library Kits

    LGC, Biosearch Technologies

    The NxSeq® AmpFREE Low DNA Library Kit allows you to build the best fragment DNA libraries possible. We optimized each step of the protocol to ensure peak performance on Illumina sequencers. In addition, these kits require only 75 ng of sheared DNA input, and produce libraries in about 2 hours…

  • Rec J Exonuclease 250 U

    LGC, Biosearch Technologies

    Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´′→3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.

  • Proteinase K 2 ml

    LGC, Biosearch Technologies

    This reagent is designed for use with the MasterPure™ kit protocols only.

  • NxSeq UltraLow DNA Library Kits

    LGC, Biosearch Technologies

    The NxSeq UltraLow DNA Library Kit is used to build DNA fragment libraries for Illumina sequencers. It requires only 50 pg to 75 ng of input DNA. This 96 rxn kit is optimized for library prep in 96-well plates and requires a NxSeq HT Dual Indexing Kit to complete library construction.

  • MasterPure DNA Purification Kit

    LGC, Biosearch Technologies

    The MasterPure™ DNA Purification Kit for Blood Version II can be used to isolate exceptionally pure genomic DNA from whole blood or buffy coat.

  • AmpliScribe™ T7 High Yield Transcription Kits are specially formulated to produce >20-fold more full-length RNA transcript than conventional in vitro transcription reactions.

  • OverExpress Chem Comp Cells

    LGC, Biosearch Technologies

    Lucigen’s OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins…

  • Tissue & Cell Lysis Solution 600 ml

    LGC, Biosearch Technologies

    This reagent is designed for use with the MasterPure™ kit protocols only.

  • CopyRight v2.0 BAC Cloning Kits

    LGC, Biosearch Technologies

    Complete your large-insert BAC cloning and library construction projects with unprecedented accuracy, reliability and efficiency using CopyRight® v2.0 BAC cloning kits with pSMART® BAC vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells. CopyRight kits are specifically…

  • Copycutter Epi400

    LGC, Biosearch Technologies

    CopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins

  • BigEasy v2.0 Lin Cloning Kits

    LGC, Biosearch Technologies

    The BigEasy Kit with the pJAZZ vector is ideal for constructing bias-free, large-insert genomic libraries, or for cloning difficult DNA of any size up to 30 kb. Because the novel pJAZZ vector is maintained as a linear molecule, the ends of the vector can rotate freely. As a result, the vector…

  • TE Buffer 70 ml

    LGC, Biosearch Technologies

    This reagent is designed for use with the MasterPure™ kit protocols only.

  • Copycontrol Htp Fosmid Library Prod Kit

    LGC, Biosearch Technologies

    Induction solution used to induce CopyControl Fosmid clones to high copy number in the TransforMax EC300 E. coli cells.

  • SelecTEV Protease

    LGC, Biosearch Technologies

    SelecTEV™ Protease is a 27 kD improved form of Tobacco Etch Virus (TEV) protease that has been engineered to be more stable, active, and specific than the native protease. SelecTEV™ Protease is a cysteine protease that recognizes the TEV recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly…

  • End-It Dna End-Repair Kits

    LGC, Biosearch Technologies

    A fast and simple enzyme blend used to create blunt ended DNA. Often used in genomic library construction and sub-cloning applications.

  • RapiDxFire Hot Start Taq DNA Polymerase

    LGC, Biosearch Technologies

    RapiDxFire™ Hot Start Taq DNA Polymerase is recombinant Taq DNA Polymerase from Thermus aquaticus bound to select blockers, which prevents unwanted, nonspecific amplification during reaction set up at temperatures less than 60 °C. Enzyme activity is restored after a short 15 second burst…

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