Reagents, G, MilliporeSigma, UN 3245
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Developed at the Broad Institute by Feng Zhang's lab, the human GeCKO v2 (two vector system) libraries consist of over 100,000 unique gRNAs for gene knock-out in the human genome. Using a dual lenti CRISPR vector wherein the pooled libraries will only express the gRNA (with the lentiGuide-Puro…
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Developed by Feng Zhang's lab at the Broad Institute, the mouse GeCKO v2 (all-in-one vector) libraries consist of over 100,000 unique gRNAs for gene knock-out in the mouse genome. Using an all-in-one lenti CRISPR vector which co-expresses Cas9 , gRNA with a puromycin selection marker, the version 2…
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Developed by Feng Zhang's lab at the Broad Institute, the human GeCKO v2 (all-in-one vector) libraries consist of over 100,000 unique gRNAs for gene knock-out in the human genome. Using an all-in-one lenti CRISPR vector which co-expresses Cas9 , gRNA with a puromycin selection marker, the version 2…